##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrFI BsrGI BssHII
BstEII BstPI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DraII DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PinAI PmaCI PmlI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 1078 GACGTC GACGT^C
AccI 757 GTATAC GT^[AC][GT]AC
AccII 972 CGCG CG^CG
AcyI 1078 GACGTC G[GA]^CG[CT]C
AhdI 917 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 989 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 100 ATTAAT AT^TAAT
AsnI 100 ATTAAT AT^TAAT
AspEI 917 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 624 GAGCTC G[AT]GC[AT]^C
AspI 323 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 488 GGATCC G^GATCC
BanI 881 GGCACC G^G[CT][GA]CC
BanII 624 GAGCTC G[GA]GC[CT]^C
BbsI 797 GTCTTC GAAGAC(2/6)
BciVI 1164 GTATCC GTATCC(6/5)
BclI 265 TGATCA T^GATCA
BfaI 167 CTAG C^TAG
BglII 771 AGATCT A^GATCT
BmyI 624 GAGCTC G[AGT]GC[ACT]^C
BpuAI 797 GTCTTC GAAGAC(2/6)
BsaHI 1078 GACGTC G[GA]^CG[CT]C
BsaI 612 GAGACC GGTCTC(1/5)
BsgI 1022 GTGCAG GTGCAG(16/14)
BsiHKAI 624 GAGCTC G[AT]GC[AT]^C
BsmBI 947 GAGACG CGTCTC(1/5)
Bsp1286I 624 GAGCTC G[AGT]GC[ACT]^C
BsrBI 877 GAGCGG CCGCTC(-3/-3)
BsrDI 891 GCAATG GCAATG(2/0)
BssSI 415 CACGAG CACGAG(-5/-1)
Bst1107I 757 GTATAC GTA^TAC
BstAPI 882 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 389 TTCGAA TT^CGAA
BstUI 972 CGCG CG^CG
BstZ17I 757 GTATAC GTA^TAC
CfoI 973 GCGC GCG^C
DraI 662 TTTAAA TTT^AAA
DraIII 710 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1072 GACTCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 70 CGGCCA [CT]^GGCC[GA]
Eam1105I 917 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 265 TGATCA T^GATCA
HhaI 973 GCGC GCG^C
Hin1I 1078 GACGTC G[GA]^CG[CT]C
HinP1I 973 GCGC G^CGC
HincII 749 GTCAAC GT[CT]^[GA]AC
HindII 749 GTCAAC GT[CT]^[GA]AC
HphI 952 GGTGA GGTGA(8/7)
MaeI 167 CTAG C^TAG
MvnI 972 CGCG CG^CG
NlaIII 712 CATG CATG^
NspV 389 TTCGAA TT^CGAA
PflFI 323 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 661 GTTTAAAC GTTT^AAAC
PshBI 100 ATTAAT AT^TAAT
SacI 624 GAGCTC GAGCT^C
SapI 1148 GAAGAGC GCTCTTC(1/4)
ScaI 306 AGTACT AGT^ACT
SfcI 1253 CTATAG C^T[GA][CT]AG
SfuI 389 TTCGAA TT^CGAA
SmlI 656 CTCAAG C^T[CT][GA]AG
Tth111I 323 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 531 TCCGGA T^CCGGA
1068 TCCGGA
AcsI 564 GAATTT [GA]^AATT[CT]
1188 AAATTC
ApoI 564 GAATTT [GA]^AATT[CT]
1188 AAATTC
Asp700I 130 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
561 GAAGAATTTC
AvaI 9 CTCGGG C^[CT]CG[GA]G
344 CCCGAG
BseAI 531 TCCGGA T^CCGGA
1068 TCCGGA
BseRI 727 CTCCTC GAGGAG(10/8)
826 CTCCTC
BsiEI 451 CGACCG CG[GA][CT]^CG
619 CGACCG
BsoBI 9 CTCGGG C^[CT]CG[GA]G
344 CCCGAG
BspEI 531 TCCGGA T^CCGGA
1068 TCCGGA
BspMI 1062 ACCTGC ACCTGC(4/8)
250 GCAGGT
BsrI 754 CCAGT ACTGG(1/-1)
922 CCAGT
BstYI 488 GGATCC [GA]^GATC[CT]
771 AGATCT
Cac8I 514 GCAAGC GC[ACGT]^[ACGT]GC
932 GCCAGC
EarI 433 CTCTTC CTCTTC(1/4)
1148 GAAGAG
Ksp632I 433 CTCTTC CTCTTC(1/4)
1148 GAAGAG
MaeII 795 ACGT A^CGT
1079 ACGT
MflI 488 GGATCC [GA]^GATC[CT]
771 AGATCT
MroI 531 TCCGGA T^CCGGA
1068 TCCGGA
MseI 101 TTAA T^TAA
663 TTAA
MspA1I 910 CAGCTG C[AC]G^C[GT]G
1025 CAGCTG
PflMI 709 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
988 CCAGACTCTGG
PvuII 910 CAGCTG CAG^CTG
1025 CAGCTG
TfiI 420 GAATC G^A[AT]TC
705 GATTC
Tru9I 101 TTAA T^TAA
663 TTAA
Tsp509I 565 AATT ^AATT
1189 AATT
TspRI 154 CACTG CA[GC]TG[ACGT][ACGT]^
382 CAGTG
Van91I 709 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
988 CCAGACTCTGG
XhoII 488 GGATCC [GA]^GATC[CT]
771 AGATCT
XmnI 130 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
561 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BpmI BsaJI BsaWI
BsiYI BslI BsmAI BsmFI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TthHB8I