##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII AflIII AgeI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
HaeII Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MflI MluI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 837 GTAGAC GT^[AC][GT]AC
AccIII 791 TCCGGA T^CCGGA
AfaI 375 GTAC GT^AC
AhdI 549 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 996 GATCC GGATC(4/5)
AocI 683 CCTGAGG CC^T[ACGT]AGG
ApaI 476 GGGCCC GGGCC^C
AspEI 549 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 498 TGCGCA TGC^GCA
BbsI 1059 GAAGAC GAAGAC(2/6)
BfaI 982 CTAG C^TAG
BpuAI 1059 GAAGAC GAAGAC(2/6)
BsaWI 791 TCCGGA [AT]^CCGG[AT]
BseAI 791 TCCGGA T^CCGGA
BseRI 913 GAGGAG GAGGAG(10/8)
BsmI 1032 GCATTC GAATGC(1/-1)
BspEI 791 TCCGGA T^CCGGA
BspMI 573 ACCTGC ACCTGC(4/8)
BssSI 458 CACGAG CACGAG(-5/-1)
Bsu36I 683 CCTGAGG CC^T[ACGT]AGG
CfoI 499 GCGC GCG^C
DpnII 996 GATC ^GATC
Eam1105I 549 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 683 CCTGAGG CC^T[ACGT]AGG
EcoNI 1154 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1205 CCATGG C^C[AT][AT]GG
FspI 498 TGCGCA TGC^GCA
HhaI 499 GCGC GCG^C
HinP1I 499 GCGC G^CGC
HindIII 1067 AAGCTT A^AGCTT
HphI 1140 GGTGA GGTGA(8/7)
MaeI 982 CTAG C^TAG
MboI 996 GATC ^GATC
MroI 791 TCCGGA T^CCGGA
MslI 441 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1205 CCATGG C^CATGG
NdeII 996 GATC ^GATC
NspI 36 GCATGT [GA]CATG^[CT]
PstI 1158 CTGCAG CTGCA^G
RsaI 375 GTAC GT^AC
Sau3AI 996 GATC ^GATC
SmlI 1064 CTCAAG C^T[CT][GA]AG
SspI 1013 AATATT AAT^ATT
StyI 1205 CCATGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 493 GCGG CCGC(-3/-1)
561 GCGG
AlwNI 569 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1182 CAGGTTCTG
AspHI 1143 GAGCTC G[AT]GC[AT]^C
1190 GAGCTC
BalI 289 TGGCCA TGG^CCA
565 TGGCCA
BpmI 659 CTCCAG CTGGAG(16/14)
1148 CTCCAG
BsiHKAI 1143 GAGCTC G[AT]GC[AT]^C
1190 GAGCTC
BstXI 661 CCAGGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
723 CCAGAGGGTTGG
DraII 475 AGGGCCC [GA]G^G[ACGT]CC[CT]
476 GGGCCCC
DsaI 384 CCGTGG C^C[GA][CT]GG
1205 CCATGG
EaeI 289 TGGCCA [CT]^GGCC[GA]
565 TGGCCA
EcoO109I 475 AGGGCCC [GA]G^G[ACGT]CC[CT]
476 GGGCCCC
HgaI 42 GACGC GACGC(5/10)
355 GACGC
MluNI 289 TGGCCA TGG^CCA
565 TGGCCA
MscI 289 TGGCCA TGG^CCA
565 TGGCCA
MwoI 36 GCATGTGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
469 GCTTCCAGGGC
PflMI 104 CCATACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
539 CCACAGCATGG
SacI 1143 GAGCTC GAGCT^C
1190 GAGCTC
SfcI 1158 CTGCAG C^T[GA][CT]AG
1179 CTACAG
TaqI 214 TCGA T^CGA
1036 TCGA
TthHB8I 214 TCGA T^CGA
1036 TCGA
Van91I 104 CCATACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
539 CCACAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AluI AvaII BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeIII MboII MnlI
MseI MspI MvaI NciI NlaIII
NlaIV PleI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI