################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AcyI AflII AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI HaeII Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 837 GTAGAC GT^[AC][GT]AC AccIII 791 TCCGGA T^CCGGA AfaI 375 GTAC GT^AC AhdI 549 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 996 GATCC GGATC(4/5) AocI 683 CCTGAGG CC^T[ACGT]AGG ApaI 476 GGGCCC GGGCC^C AspEI 549 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 498 TGCGCA TGC^GCA BbsI 1059 GAAGAC GAAGAC(2/6) BfaI 982 CTAG C^TAG BpuAI 1059 GAAGAC GAAGAC(2/6) BsaWI 791 TCCGGA [AT]^CCGG[AT] BseAI 791 TCCGGA T^CCGGA BseRI 913 GAGGAG GAGGAG(10/8) BsmI 1032 GCATTC GAATGC(1/-1) BspEI 791 TCCGGA T^CCGGA BspMI 573 ACCTGC ACCTGC(4/8) BssSI 458 CACGAG CACGAG(-5/-1) Bsu36I 683 CCTGAGG CC^T[ACGT]AGG CfoI 499 GCGC GCG^C DpnII 996 GATC ^GATC Eam1105I 549 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 683 CCTGAGG CC^T[ACGT]AGG EcoNI 1154 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1205 CCATGG C^C[AT][AT]GG FspI 498 TGCGCA TGC^GCA HhaI 499 GCGC GCG^C HinP1I 499 GCGC G^CGC HindIII 1067 AAGCTT A^AGCTT HphI 1140 GGTGA GGTGA(8/7) MaeI 982 CTAG C^TAG MboI 996 GATC ^GATC MroI 791 TCCGGA T^CCGGA MslI 441 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1205 CCATGG C^CATGG NdeII 996 GATC ^GATC NspI 36 GCATGT [GA]CATG^[CT] PstI 1158 CTGCAG CTGCA^G RsaI 375 GTAC GT^AC Sau3AI 996 GATC ^GATC SmlI 1064 CTCAAG C^T[CT][GA]AG SspI 1013 AATATT AAT^ATT StyI 1205 CCATGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 493 GCGG CCGC(-3/-1) 561 GCGG AlwNI 569 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 1182 CAGGTTCTG AspHI 1143 GAGCTC G[AT]GC[AT]^C 1190 GAGCTC BalI 289 TGGCCA TGG^CCA 565 TGGCCA BpmI 659 CTCCAG CTGGAG(16/14) 1148 CTCCAG BsiHKAI 1143 GAGCTC G[AT]GC[AT]^C 1190 GAGCTC BstXI 661 CCAGGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 723 CCAGAGGGTTGG DraII 475 AGGGCCC [GA]G^G[ACGT]CC[CT] 476 GGGCCCC DsaI 384 CCGTGG C^C[GA][CT]GG 1205 CCATGG EaeI 289 TGGCCA [CT]^GGCC[GA] 565 TGGCCA EcoO109I 475 AGGGCCC [GA]G^G[ACGT]CC[CT] 476 GGGCCCC HgaI 42 GACGC GACGC(5/10) 355 GACGC MluNI 289 TGGCCA TGG^CCA 565 TGGCCA MscI 289 TGGCCA TGG^CCA 565 TGGCCA MwoI 36 GCATGTGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 469 GCTTCCAGGGC PflMI 104 CCATACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 539 CCACAGCATGG SacI 1143 GAGCTC GAGCT^C 1190 GAGCTC SfcI 1158 CTGCAG C^T[GA][CT]AG 1179 CTACAG TaqI 214 TCGA T^CGA 1036 TCGA TthHB8I 214 TCGA T^CGA 1036 TCGA Van91I 104 CCATACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 539 CCACAGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AluI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeIII MboII MnlI MseI MspI MvaI NciI NlaIII NlaIV PleI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI