##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcsI        
AcyI        AflII       AflIII      AgeI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvaI        
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EarI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
HaeII       Hin1I       HincII      HindII      HpaI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MfeI        MflI        MluI        MspA1I      
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          837  GTAGAC               GT^[AC][GT]AC
AccIII        791  TCCGGA               T^CCGGA
AfaI          375  GTAC                 GT^AC
AhdI          549  GACTATGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          996  GATCC                GGATC(4/5)
AocI          683  CCTGAGG              CC^T[ACGT]AGG
ApaI          476  GGGCCC               GGGCC^C
AspEI         549  GACTATGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         498  TGCGCA               TGC^GCA
BbsI         1059  GAAGAC               GAAGAC(2/6)
BfaI          982  CTAG                 C^TAG
BpuAI        1059  GAAGAC               GAAGAC(2/6)
BsaWI         791  TCCGGA               [AT]^CCGG[AT]
BseAI         791  TCCGGA               T^CCGGA
BseRI         913  GAGGAG               GAGGAG(10/8)
BsmI         1032  GCATTC               GAATGC(1/-1)
BspEI         791  TCCGGA               T^CCGGA
BspMI         573  ACCTGC               ACCTGC(4/8)
BssSI         458  CACGAG               CACGAG(-5/-1)
Bsu36I        683  CCTGAGG              CC^T[ACGT]AGG
CfoI          499  GCGC                 GCG^C
DpnII         996  GATC                 ^GATC
Eam1105I      549  GACTATGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        683  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1154  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      1205  CCATGG               C^C[AT][AT]GG
FspI          498  TGCGCA               TGC^GCA
HhaI          499  GCGC                 GCG^C
HinP1I        499  GCGC                 G^CGC
HindIII      1067  AAGCTT               A^AGCTT
HphI         1140  GGTGA                GGTGA(8/7)
MaeI          982  CTAG                 C^TAG
MboI          996  GATC                 ^GATC
MroI          791  TCCGGA               T^CCGGA
MslI          441  CATCACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         1205  CCATGG               C^CATGG
NdeII         996  GATC                 ^GATC
NspI           36  GCATGT               [GA]CATG^[CT]
PstI         1158  CTGCAG               CTGCA^G
RsaI          375  GTAC                 GT^AC
Sau3AI        996  GATC                 ^GATC
SmlI         1064  CTCAAG               C^T[CT][GA]AG
SspI         1013  AATATT               AAT^ATT
StyI         1205  CCATGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI          493  GCGG                 CCGC(-3/-1)
              561  GCGG                
AlwNI         569  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1182  CAGGTTCTG           
AspHI        1143  GAGCTC               G[AT]GC[AT]^C
             1190  GAGCTC              
BalI          289  TGGCCA               TGG^CCA
              565  TGGCCA              
BpmI          659  CTCCAG               CTGGAG(16/14)
             1148  CTCCAG              
BsiHKAI      1143  GAGCTC               G[AT]GC[AT]^C
             1190  GAGCTC              
BstXI         661  CCAGGTCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              723  CCAGAGGGTTGG        
DraII         475  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              476  GGGCCCC             
DsaI          384  CCGTGG               C^C[GA][CT]GG
             1205  CCATGG              
EaeI          289  TGGCCA               [CT]^GGCC[GA]
              565  TGGCCA              
EcoO109I      475  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              476  GGGCCCC             
HgaI           42  GACGC                GACGC(5/10)
              355  GACGC               
MluNI         289  TGGCCA               TGG^CCA
              565  TGGCCA              
MscI          289  TGGCCA               TGG^CCA
              565  TGGCCA              
MwoI           36  GCATGTGACGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              469  GCTTCCAGGGC         
PflMI         104  CCATACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              539  CCACAGCATGG         
SacI         1143  GAGCTC               GAGCT^C
             1190  GAGCTC              
SfcI         1158  CTGCAG               C^T[GA][CT]AG
             1179  CTACAG              
TaqI          214  TCGA                 T^CGA
             1036  TCGA                
TthHB8I       214  TCGA                 T^CGA
             1036  TCGA                
Van91I        104  CCATACTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              539  CCACAGCATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AluI        AvaII       BanII       BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        MaeIII      MboII       MnlI        
MseI        MspI        MvaI        NciI        NlaIII      
NlaIV       PleI        Sau96I      ScrFI       SfaNI       
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI