##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BpmI BpuAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DsaI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HgaI HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SpeI
SphI Sse8387I SspI StyI SwaI
Tth111I XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 521 GACGTC GACGT^C
AcsI 410 GAATTC [GA]^AATT[CT]
AcyI 521 GACGTC G[GA]^CG[CT]C
AhdI 942 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 548 GATCC GGATC(4/5)
ApoI 410 GAATTC [GA]^AATT[CT]
AspEI 942 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1059 CCCGGG C^[CT]CG[GA]G
AvaII 674 GGACC G^G[AT]CC
BlpI 690 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 690 GCTGAGC GC^T[ACGT]AGC
BsaAI 183 TACGTA [CT]AC^GT[GA]
BsaHI 521 GACGTC G[GA]^CG[CT]C
BsgI 1036 GTGCAG GTGCAG(16/14)
BsmFI 591 GGGAC GGGAC(10/14)
BsoBI 1059 CCCGGG C^[CT]CG[GA]G
Bsp1407I 559 TGTACA T^GTACA
BspMI 511 GCAGGT ACCTGC(4/8)
BsrBI 1019 CCGCTC CCGCTC(-3/-3)
BsrGI 559 TGTACA T^GTACA
BstXI 94 CCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 690 GCTGAGC GC^T[ACGT]AGC
DraII 673 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 675 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 745 TGGCCG [CT]^GGCC[GA]
Eam1105I 942 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 116 CCTGATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 673 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 410 GAATTC G^AATTC
HapII 1060 CCGG C^CGG
Hin1I 521 GACGTC G[GA]^CG[CT]C
HincII 834 GTCGAC GT[CT]^[GA]AC
HindII 834 GTCGAC GT[CT]^[GA]AC
HpaII 1060 CCGG C^CGG
MspI 1060 CCGG C^CGG
NlaIII 418 CATG CATG^
NlaIV 674 GGACCC GG[ACGT]^[ACGT]CC
PflMI 1118 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 673 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 609 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 771 CAGCTG CAG^CTG
SalI 834 GTCGAC G^TCGAC
SmaI 1059 CCCGGG CCC^GGG
SnaBI 183 TACGTA TAC^GTA
SspBI 559 TGTACA T^GTACA
StuI 844 AGGCCT AGG^CCT
Van91I 1118 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 878 TCTAGA T^CTAGA
XmaCI 1059 CCCGGG C^CCGGG
XmaI 1059 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccI 255 GTCTAC GT^[AC][GT]AC
834 GTCGAC
AfaI 560 GTAC GT^AC
972 GTAC
AlwNI 247 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
484 CAGAGGCTG
AocI 663 CCTGAGG CC^T[ACGT]AGG
678 CCTGAGG
AspHI 216 GAGCTC G[AT]GC[AT]^C
1064 GAGCTC
BanII 216 GAGCTC G[GA]GC[CT]^C
1064 GAGCTC
BclI 272 TGATCA T^GATCA
710 TGATCA
BcnI 1059 CCCGG CC^[GC]GG
1060 CCGGG
BfaI 63 CTAG C^TAG
879 CTAG
BmyI 216 GAGCTC G[AGT]GC[ACT]^C
1064 GAGCTC
BsaJI 670 CCCAGG C^C[ACGT][ACGT]GG
1059 CCCGGG
BsiHKAI 216 GAGCTC G[AT]GC[AT]^C
1064 GAGCTC
Bsp1286I 216 GAGCTC G[AGT]GC[ACT]^C
1064 GAGCTC
BsrI 743 ACTGG ACTGG(1/-1)
208 CCAGT
BstNI 671 CCAGG CC^[AT]GG
847 CCTGG
Bsu36I 663 CCTGAGG CC^T[ACGT]AGG
678 CCTGAGG
Cac8I 52 GCTTGC GC[ACGT]^[ACGT]GC
1008 GCAGGC
CfoI 239 GCGC GCG^C
1056 GCGC
Cfr13I 668 GGCCC G^G[ACGT]CC
674 GGACC
EarI 874 CTCTTC CTCTTC(1/4)
1149 GAAGAG
Eco81I 663 CCTGAGG CC^T[ACGT]AGG
678 CCTGAGG
EcoRII 671 CCAGG ^CC[AT]GG
847 CCTGG
FbaI 272 TGATCA T^GATCA
710 TGATCA
HhaI 239 GCGC GCG^C
1056 GCGC
HinP1I 239 GCGC G^CGC
1056 GCGC
HindIII 50 AAGCTT A^AGCTT
261 AAGCTT
Ksp632I 874 CTCTTC CTCTTC(1/4)
1149 GAAGAG
MaeI 63 CTAG C^TAG
879 CTAG
MseI 42 TTAA T^TAA
1075 TTAA
MspA1I 771 CAGCTG C[AC]G^C[GT]G
1045 CAGCGG
MvaI 671 CCAGG CC^[AT]GG
847 CCTGG
NciI 1059 CCCGG CC^[GC]GG
1060 CCGGG
PleI 630 GAGTC GAGTC(4/5)
948 GAGTC
PstI 481 CTGCAG CTGCA^G
623 CTGCAG
RsaI 560 GTAC GT^AC
972 GTAC
SacI 216 GAGCTC GAGCT^C
1064 GAGCTC
Sau96I 668 GGCCC G^G[ACGT]CC
674 GGACC
SfaNI 890 GATGC GCATC(5/9)
138 GCATC
TaqI 190 TCGA T^CGA
835 TCGA
Tru9I 42 TTAA T^TAA
1075 TTAA
TthHB8I 190 TCGA T^CGA
835 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BsmAI BssKI BstF5I DdeI DpnII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MwoI NdeII Sau3AI ScrFI
SfcI SmlI TfiI TseI Tsp45I
Tsp509I TspRI