################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BpmI BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Cfr10I ClaI CpoI DraI DraIII DsaI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SpeI SphI Sse8387I SspI StyI SwaI Tth111I XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 521 GACGTC GACGT^C AcsI 410 GAATTC [GA]^AATT[CT] AcyI 521 GACGTC G[GA]^CG[CT]C AhdI 942 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 548 GATCC GGATC(4/5) ApoI 410 GAATTC [GA]^AATT[CT] AspEI 942 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1059 CCCGGG C^[CT]CG[GA]G AvaII 674 GGACC G^G[AT]CC BlpI 690 GCTGAGC GC^T[ACGT]AGC Bpu1102I 690 GCTGAGC GC^T[ACGT]AGC BsaAI 183 TACGTA [CT]AC^GT[GA] BsaHI 521 GACGTC G[GA]^CG[CT]C BsgI 1036 GTGCAG GTGCAG(16/14) BsmFI 591 GGGAC GGGAC(10/14) BsoBI 1059 CCCGGG C^[CT]CG[GA]G Bsp1407I 559 TGTACA T^GTACA BspMI 511 GCAGGT ACCTGC(4/8) BsrBI 1019 CCGCTC CCGCTC(-3/-3) BsrGI 559 TGTACA T^GTACA BstXI 94 CCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 690 GCTGAGC GC^T[ACGT]AGC DraII 673 AGGACCC [GA]G^G[ACGT]CC[CT] DrdI 675 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 745 TGGCCG [CT]^GGCC[GA] Eam1105I 942 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 116 CCTGATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 673 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 410 GAATTC G^AATTC HapII 1060 CCGG C^CGG Hin1I 521 GACGTC G[GA]^CG[CT]C HincII 834 GTCGAC GT[CT]^[GA]AC HindII 834 GTCGAC GT[CT]^[GA]AC HpaII 1060 CCGG C^CGG MspI 1060 CCGG C^CGG NlaIII 418 CATG CATG^ NlaIV 674 GGACCC GG[ACGT]^[ACGT]CC PflMI 1118 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 673 AGGACCC [GA]G^G[AT]CC[CT] PshAI 609 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 771 CAGCTG CAG^CTG SalI 834 GTCGAC G^TCGAC SmaI 1059 CCCGGG CCC^GGG SnaBI 183 TACGTA TAC^GTA SspBI 559 TGTACA T^GTACA StuI 844 AGGCCT AGG^CCT Van91I 1118 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 878 TCTAGA T^CTAGA XmaCI 1059 CCCGGG C^CCGGG XmaI 1059 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AccI 255 GTCTAC GT^[AC][GT]AC 834 GTCGAC AfaI 560 GTAC GT^AC 972 GTAC AlwNI 247 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG 484 CAGAGGCTG AocI 663 CCTGAGG CC^T[ACGT]AGG 678 CCTGAGG AspHI 216 GAGCTC G[AT]GC[AT]^C 1064 GAGCTC BanII 216 GAGCTC G[GA]GC[CT]^C 1064 GAGCTC BclI 272 TGATCA T^GATCA 710 TGATCA BcnI 1059 CCCGG CC^[GC]GG 1060 CCGGG BfaI 63 CTAG C^TAG 879 CTAG BmyI 216 GAGCTC G[AGT]GC[ACT]^C 1064 GAGCTC BsaJI 670 CCCAGG C^C[ACGT][ACGT]GG 1059 CCCGGG BsiHKAI 216 GAGCTC G[AT]GC[AT]^C 1064 GAGCTC Bsp1286I 216 GAGCTC G[AGT]GC[ACT]^C 1064 GAGCTC BsrI 743 ACTGG ACTGG(1/-1) 208 CCAGT BstNI 671 CCAGG CC^[AT]GG 847 CCTGG Bsu36I 663 CCTGAGG CC^T[ACGT]AGG 678 CCTGAGG Cac8I 52 GCTTGC GC[ACGT]^[ACGT]GC 1008 GCAGGC CfoI 239 GCGC GCG^C 1056 GCGC Cfr13I 668 GGCCC G^G[ACGT]CC 674 GGACC EarI 874 CTCTTC CTCTTC(1/4) 1149 GAAGAG Eco81I 663 CCTGAGG CC^T[ACGT]AGG 678 CCTGAGG EcoRII 671 CCAGG ^CC[AT]GG 847 CCTGG FbaI 272 TGATCA T^GATCA 710 TGATCA HhaI 239 GCGC GCG^C 1056 GCGC HinP1I 239 GCGC G^CGC 1056 GCGC HindIII 50 AAGCTT A^AGCTT 261 AAGCTT Ksp632I 874 CTCTTC CTCTTC(1/4) 1149 GAAGAG MaeI 63 CTAG C^TAG 879 CTAG MseI 42 TTAA T^TAA 1075 TTAA MspA1I 771 CAGCTG C[AC]G^C[GT]G 1045 CAGCGG MvaI 671 CCAGG CC^[AT]GG 847 CCTGG NciI 1059 CCCGG CC^[GC]GG 1060 CCGGG PleI 630 GAGTC GAGTC(4/5) 948 GAGTC PstI 481 CTGCAG CTGCA^G 623 CTGCAG RsaI 560 GTAC GT^AC 972 GTAC SacI 216 GAGCTC GAGCT^C 1064 GAGCTC Sau96I 668 GGCCC G^G[ACGT]CC 674 GGACC SfaNI 890 GATGC GCATC(5/9) 138 GCATC TaqI 190 TCGA T^CGA 835 TCGA Tru9I 42 TTAA T^TAA 1075 TTAA TthHB8I 190 TCGA T^CGA 835 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BsiYI BslI BsmAI BssKI BstF5I DdeI DpnII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MwoI NdeII Sau3AI ScrFI SfcI SmlI TfiI TseI Tsp45I Tsp509I TspRI