################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AspEI AspI BbeI BbrPI BbvCI BcgI BclI BglI BsaAI BsiWI BsmI BspDI BspHI BssHII BstUI ClaI CpoI DrdI Eam1105I Eco47III EcoRV EcoT22I FbaI FseI HindIII HpaI KasI KspI MfeI MluI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PstI PvuI RcaI RsrII SacII SapI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 1508 GGTACC G^GTACC AccIII 1035 TCCGGA T^CCGGA AcsI 3649 GAATTC [GA]^AATT[CT] AcyI 413 GACGCC G[GA]^CG[CT]C AflII 3454 CTTAAG C^TTAAG ApoI 3649 GAATTC [GA]^AATT[CT] AseI 2096 ATTAAT AT^TAAT AsnI 2096 ATTAAT AT^TAAT Asp718I 1508 GGTACC G^GTACC AviII 2665 TGCGCA TGC^GCA BamHI 537 GGATCC G^GATCC BfrI 3454 CTTAAG C^TTAAG BglII 1563 AGATCT A^GATCT BlpI 1650 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1650 GCTCAGC GC^T[ACGT]AGC BsaBI 2375 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 413 GACGCC G[GA]^CG[CT]C BsaI 3205 GGTCTC GGTCTC(1/5) BseAI 1035 TCCGGA T^CCGGA BsmBI 297 GAGACG CGTCTC(1/5) BspEI 1035 TCCGGA T^CCGGA BspMI 2418 ACCTGC ACCTGC(4/8) BsrBI 2087 GAGCGG CCGCTC(-3/-3) Bst1107I 2252 GTATAC GTA^TAC BstBI 2034 TTCGAA TT^CGAA BstEII 2917 GGTTACC G^GT[ACGT]ACC BstPI 2917 GGTTACC G^GT[ACGT]ACC BstZ17I 2252 GTATAC GTA^TAC CelII 1650 GCTCAGC GC^T[ACGT]AGC DraI 815 TTTAAA TTT^AAA DsaI 3774 CCACGG C^C[GA][CT]GG EagI 3200 CGGCCG C^GGCCG EclXI 3200 CGGCCG C^GGCCG Eco52I 3200 CGGCCG C^GGCCG EcoO65I 2917 GGTTACC G^GT[ACGT]ACC EcoRI 3649 GAATTC G^AATTC FspI 2665 TGCGCA TGC^GCA HaeII 117 AGCGCC [GA]GCGC^[CT] Hin1I 413 GACGCC G[GA]^CG[CT]C KpnI 1508 GGTACC GGTAC^C MaeII 1150 ACGT A^CGT MamI 2375 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1035 TCCGGA T^CCGGA NspV 2034 TTCGAA TT^CGAA PpuMI 444 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 1843 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2096 ATTAAT AT^TAAT SalI 3510 GTCGAC G^TCGAC ScaI 1206 AGTACT AGT^ACT SfuI 2034 TTCGAA TT^CGAA SmaI 3319 CCCGGG CCC^GGG StuI 1053 AGGCCT AGG^CCT XmaCI 3319 CCCGGG C^CCGGG XmaI 3319 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflIII 747 ACATGT A^C[GA][CT]GT 1269 ACATGT ApaI 3314 GGGCCC GGGCC^C 4135 GGGCCC AvrII 1972 CCTAGG C^CTAGG 4140 CCTAGG BbsI 1016 GAAGAC GAAGAC(2/6) 485 GTCTTC BlnI 1972 CCTAGG C^CTAGG 4140 CCTAGG BpuAI 1016 GAAGAC GAAGAC(2/6) 485 GTCTTC BsiEI 3066 CGACCG CG[GA][CT]^CG 3200 CGGCCG BspLU11I 747 ACATGT A^CATGT 1269 ACATGT BssSI 2546 CACGAG CACGAG(-5/-1) 2294 CTCGTG BstAPI 854 GCATCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3439 GCACCCAGTGC HincII 452 GTCAAC GT[CT]^[GA]AC 3510 GTCGAC HindII 452 GTCAAC GT[CT]^[GA]AC 3510 GTCGAC NdeI 1806 CATATG CA^TATG 2661 CATATG PvuII 3686 CAGCTG CAG^CTG 3984 CAGCTG SacI 9 GAGCTC GAGCT^C 3283 GAGCTC SfcI 1187 CTACAG C^T[GA][CT]AG 1754 CTACAG SmlI 2312 CTTGAG C^T[CT][GA]AG 3454 CTTAAG XcmI 2187 CCAATCGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3421 CCAGCCCAGTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AlwNI AocI Asp700I AspHI AvaI AvaII BalI BanI BanII BbvI BciVI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI