##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AspEI
AspI BbeI BbrPI BbvCI BcgI
BclI BglI BsaAI BsiWI BsmI
BspDI BspHI BssHII BstUI ClaI
CpoI DrdI Eam1105I Eco47III EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI KspI MfeI MluI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PstI PvuI RcaI
RsrII SacII SapI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1508 GGTACC G^GTACC
AccIII 1035 TCCGGA T^CCGGA
AcsI 3649 GAATTC [GA]^AATT[CT]
AcyI 413 GACGCC G[GA]^CG[CT]C
AflII 3454 CTTAAG C^TTAAG
ApoI 3649 GAATTC [GA]^AATT[CT]
AseI 2096 ATTAAT AT^TAAT
AsnI 2096 ATTAAT AT^TAAT
Asp718I 1508 GGTACC G^GTACC
AviII 2665 TGCGCA TGC^GCA
BamHI 537 GGATCC G^GATCC
BfrI 3454 CTTAAG C^TTAAG
BglII 1563 AGATCT A^GATCT
BlpI 1650 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1650 GCTCAGC GC^T[ACGT]AGC
BsaBI 2375 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 413 GACGCC G[GA]^CG[CT]C
BsaI 3205 GGTCTC GGTCTC(1/5)
BseAI 1035 TCCGGA T^CCGGA
BsmBI 297 GAGACG CGTCTC(1/5)
BspEI 1035 TCCGGA T^CCGGA
BspMI 2418 ACCTGC ACCTGC(4/8)
BsrBI 2087 GAGCGG CCGCTC(-3/-3)
Bst1107I 2252 GTATAC GTA^TAC
BstBI 2034 TTCGAA TT^CGAA
BstEII 2917 GGTTACC G^GT[ACGT]ACC
BstPI 2917 GGTTACC G^GT[ACGT]ACC
BstZ17I 2252 GTATAC GTA^TAC
CelII 1650 GCTCAGC GC^T[ACGT]AGC
DraI 815 TTTAAA TTT^AAA
DsaI 3774 CCACGG C^C[GA][CT]GG
EagI 3200 CGGCCG C^GGCCG
EclXI 3200 CGGCCG C^GGCCG
Eco52I 3200 CGGCCG C^GGCCG
EcoO65I 2917 GGTTACC G^GT[ACGT]ACC
EcoRI 3649 GAATTC G^AATTC
FspI 2665 TGCGCA TGC^GCA
HaeII 117 AGCGCC [GA]GCGC^[CT]
Hin1I 413 GACGCC G[GA]^CG[CT]C
KpnI 1508 GGTACC GGTAC^C
MaeII 1150 ACGT A^CGT
MamI 2375 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1035 TCCGGA T^CCGGA
NspV 2034 TTCGAA TT^CGAA
PpuMI 444 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1843 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2096 ATTAAT AT^TAAT
SalI 3510 GTCGAC G^TCGAC
ScaI 1206 AGTACT AGT^ACT
SfuI 2034 TTCGAA TT^CGAA
SmaI 3319 CCCGGG CCC^GGG
StuI 1053 AGGCCT AGG^CCT
XmaCI 3319 CCCGGG C^CCGGG
XmaI 3319 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 747 ACATGT A^C[GA][CT]GT
1269 ACATGT
ApaI 3314 GGGCCC GGGCC^C
4135 GGGCCC
AvrII 1972 CCTAGG C^CTAGG
4140 CCTAGG
BbsI 1016 GAAGAC GAAGAC(2/6)
485 GTCTTC
BlnI 1972 CCTAGG C^CTAGG
4140 CCTAGG
BpuAI 1016 GAAGAC GAAGAC(2/6)
485 GTCTTC
BsiEI 3066 CGACCG CG[GA][CT]^CG
3200 CGGCCG
BspLU11I 747 ACATGT A^CATGT
1269 ACATGT
BssSI 2546 CACGAG CACGAG(-5/-1)
2294 CTCGTG
BstAPI 854 GCATCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3439 GCACCCAGTGC
HincII 452 GTCAAC GT[CT]^[GA]AC
3510 GTCGAC
HindII 452 GTCAAC GT[CT]^[GA]AC
3510 GTCGAC
NdeI 1806 CATATG CA^TATG
2661 CATATG
PvuII 3686 CAGCTG CAG^CTG
3984 CAGCTG
SacI 9 GAGCTC GAGCT^C
3283 GAGCTC
SfcI 1187 CTACAG C^T[GA][CT]AG
1754 CTACAG
SmlI 2312 CTTGAG C^T[CT][GA]AG
3454 CTTAAG
XcmI 2187 CCAATCGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3421 CCAGCCCAGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbvI
BciVI BcnI BfaI BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI