##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspHI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NsiI NspI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfaNI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI TthHB8I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 657 GGTACC G^GTACC
AccII 20 CGCG CG^CG
AgeI 824 ACCGGT A^CCGGT
AhdI 775 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 564 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 314 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 657 GGTACC G^GTACC
AspEI 775 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 758 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 213 CCCGGG C^[CT]CG[GA]G
AvaII 560 GGACC G^G[AT]CC
BamHI 620 GGATCC G^GATCC
BanII 174 GAGCCC G[GA]GC[CT]^C
BbsI 231 GAAGAC GAAGAC(2/6)
BbvCI 503 CCTCAGC CCTCAGC(-5/-2)
BglI 375 GCCACGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 125 AGATCT A^GATCT
BpmI 420 CTGGAG CTGGAG(16/14)
BpuAI 231 GAAGAC GAAGAC(2/6)
BsaI 190 GAGACC GGTCTC(1/5)
BseRI 39 GAGGAG GAGGAG(10/8)
BsmBI 594 CGTCTC CGTCTC(1/5)
BsoBI 213 CCCGGG C^[CT]CG[GA]G
BspHI 845 TCATGA T^CATGA
BsrBI 648 CCGCTC CCGCTC(-3/-3)
BsrDI 496 GCAATG GCAATG(2/0)
BsrI 242 CCAGT ACTGG(1/-1)
BstF5I 140 CATCC GGATG(2/0)
BstUI 20 CGCG CG^CG
CfoI 281 GCGC GCG^C
DraII 565 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 775 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 597 CTCTTC CTCTTC(1/4)
EcoO109I 565 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 790 CCATGG C^C[AT][AT]GG
FokI 140 CATCC GGATG(9/13)
HaeII 280 AGCGCC [GA]GCGC^[CT]
HhaI 281 GCGC GCG^C
HinP1I 281 GCGC G^CGC
HindIII 364 AAGCTT A^AGCTT
HinfI 641 GACTC G^A[ACGT]TC
KpnI 657 GGTACC GGTAC^C
Ksp632I 597 CTCTTC CTCTTC(1/4)
MseI 835 TTAA T^TAA
MspA1I 309 CAGCTG C[AC]G^C[GT]G
MvnI 20 CGCG CG^CG
NcoI 790 CCATGG C^CATGG
NruI 19 TCGCGA TCG^CGA
PflFI 758 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 824 ACCGGT A^CCGGT
PleI 641 GACTC GAGTC(4/5)
PstI 636 CTGCAG CTGCA^G
PvuII 309 CAGCTG CAG^CTG
RcaI 845 TCATGA T^CATGA
SexAI 193 ACCTGGT A^CC[AT]GGT
SfcI 636 CTGCAG C^T[GA][CT]AG
SgrAI 823 CACCGGTG C[GA]^CCGG[CT]G
SmaI 213 CCCGGG CCC^GGG
StyI 790 CCATGG C^C[AT][AT]GG
Tru9I 835 TTAA T^TAA
Tth111I 758 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 213 CCCGGG C^CCGGG
XmaI 213 CCCGGG C^CCGGG
XmnI 314 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BfaI 7 CTAG C^TAG
895 CTAG
BsmFI 59 GTCCC GGGAC(10/14)
783 GTCCC
BsrFI 200 GCCGGT [GA]^CCGG[CT]
824 ACCGGT
BstXI 607 CCATCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
661 CCATTGTCATGG
BstYI 125 AGATCT [GA]^GATC[CT]
620 GGATCC
Cfr10I 200 GCCGGT [GA]^CCGG[CT]
824 ACCGGT
DsaI 376 CCACGG C^C[GA][CT]GG
790 CCATGG
EcoRI 516 GAATTC G^AATTC
874 GAATTC
FauI 143 CCCGC CCCGC(4/6)
647 CCCGC
MaeI 7 CTAG C^TAG
895 CTAG
MflI 125 AGATCT [GA]^GATC[CT]
620 GGATCC
MslI 662 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
760 CACTGTCATG
SmlI 129 CTTGAG C^T[CT][GA]AG
261 CTCAAG
TspRI 760 CACTG CA[GC]TG[ACGT][ACGT]^
913 CACTG
XcmI 75 CCAAATTTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
680 CCACCGTGACTGTGG
XhoII 125 AGATCT [GA]^GATC[CT]
620 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanI BbvI BcnI BmyI
BsaJI BsaWI BsiYI BslI BsmAI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HapII HgaI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TseI Tsp45I Tsp509I