################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspHI AviII AvrII BalI BbeI BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HpaI KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI TthHB8I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### Acc65I 657 GGTACC G^GTACC AccII 20 CGCG CG^CG AgeI 824 ACCGGT A^CCGGT AhdI 775 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 564 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 314 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 657 GGTACC G^GTACC AspEI 775 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 758 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 213 CCCGGG C^[CT]CG[GA]G AvaII 560 GGACC G^G[AT]CC BamHI 620 GGATCC G^GATCC BanII 174 GAGCCC G[GA]GC[CT]^C BbsI 231 GAAGAC GAAGAC(2/6) BbvCI 503 CCTCAGC CCTCAGC(-5/-2) BglI 375 GCCACGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 125 AGATCT A^GATCT BpmI 420 CTGGAG CTGGAG(16/14) BpuAI 231 GAAGAC GAAGAC(2/6) BsaI 190 GAGACC GGTCTC(1/5) BseRI 39 GAGGAG GAGGAG(10/8) BsmBI 594 CGTCTC CGTCTC(1/5) BsoBI 213 CCCGGG C^[CT]CG[GA]G BspHI 845 TCATGA T^CATGA BsrBI 648 CCGCTC CCGCTC(-3/-3) BsrDI 496 GCAATG GCAATG(2/0) BsrI 242 CCAGT ACTGG(1/-1) BstF5I 140 CATCC GGATG(2/0) BstUI 20 CGCG CG^CG CfoI 281 GCGC GCG^C DraII 565 AGGGCCT [GA]G^G[ACGT]CC[CT] Eam1105I 775 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 597 CTCTTC CTCTTC(1/4) EcoO109I 565 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 790 CCATGG C^C[AT][AT]GG FokI 140 CATCC GGATG(9/13) HaeII 280 AGCGCC [GA]GCGC^[CT] HhaI 281 GCGC GCG^C HinP1I 281 GCGC G^CGC HindIII 364 AAGCTT A^AGCTT HinfI 641 GACTC G^A[ACGT]TC KpnI 657 GGTACC GGTAC^C Ksp632I 597 CTCTTC CTCTTC(1/4) MseI 835 TTAA T^TAA MspA1I 309 CAGCTG C[AC]G^C[GT]G MvnI 20 CGCG CG^CG NcoI 790 CCATGG C^CATGG NruI 19 TCGCGA TCG^CGA PflFI 758 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 824 ACCGGT A^CCGGT PleI 641 GACTC GAGTC(4/5) PstI 636 CTGCAG CTGCA^G PvuII 309 CAGCTG CAG^CTG RcaI 845 TCATGA T^CATGA SexAI 193 ACCTGGT A^CC[AT]GGT SfcI 636 CTGCAG C^T[GA][CT]AG SgrAI 823 CACCGGTG C[GA]^CCGG[CT]G SmaI 213 CCCGGG CCC^GGG StyI 790 CCATGG C^C[AT][AT]GG Tru9I 835 TTAA T^TAA Tth111I 758 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 213 CCCGGG C^CCGGG XmaI 213 CCCGGG C^CCGGG XmnI 314 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BfaI 7 CTAG C^TAG 895 CTAG BsmFI 59 GTCCC GGGAC(10/14) 783 GTCCC BsrFI 200 GCCGGT [GA]^CCGG[CT] 824 ACCGGT BstXI 607 CCATCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 661 CCATTGTCATGG BstYI 125 AGATCT [GA]^GATC[CT] 620 GGATCC Cfr10I 200 GCCGGT [GA]^CCGG[CT] 824 ACCGGT DsaI 376 CCACGG C^C[GA][CT]GG 790 CCATGG EcoRI 516 GAATTC G^AATTC 874 GAATTC FauI 143 CCCGC CCCGC(4/6) 647 CCCGC MaeI 7 CTAG C^TAG 895 CTAG MflI 125 AGATCT [GA]^GATC[CT] 620 GGATCC MslI 662 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 760 CACTGTCATG SmlI 129 CTTGAG C^T[CT][GA]AG 261 CTCAAG TspRI 760 CACTG CA[GC]TG[ACGT][ACGT]^ 913 CACTG XcmI 75 CCAAATTTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 680 CCACCGTGACTGTGG XhoII 125 AGATCT [GA]^GATC[CT] 620 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BanI BbvI BcnI BmyI BsaJI BsaWI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HgaI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI Tsp45I Tsp509I