##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AspHI       AviII       AvrII       BalI        BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HincII      HindII      HpaI        
KasI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfaNI       SfiI        SfoI        
SfuI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        TthHB8I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

Acc65I        657  GGTACC               G^GTACC
AccII          20  CGCG                 CG^CG
AgeI          824  ACCGGT               A^CCGGT
AhdI          775  GACCTTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         564  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       314  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       657  GGTACC               G^GTACC
AspEI         775  GACCTTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          758  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          213  CCCGGG               C^[CT]CG[GA]G
AvaII         560  GGACC                G^G[AT]CC
BamHI         620  GGATCC               G^GATCC
BanII         174  GAGCCC               G[GA]GC[CT]^C
BbsI          231  GAAGAC               GAAGAC(2/6)
BbvCI         503  CCTCAGC              CCTCAGC(-5/-2)
BglI          375  GCCACGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         125  AGATCT               A^GATCT
BpmI          420  CTGGAG               CTGGAG(16/14)
BpuAI         231  GAAGAC               GAAGAC(2/6)
BsaI          190  GAGACC               GGTCTC(1/5)
BseRI          39  GAGGAG               GAGGAG(10/8)
BsmBI         594  CGTCTC               CGTCTC(1/5)
BsoBI         213  CCCGGG               C^[CT]CG[GA]G
BspHI         845  TCATGA               T^CATGA
BsrBI         648  CCGCTC               CCGCTC(-3/-3)
BsrDI         496  GCAATG               GCAATG(2/0)
BsrI          242  CCAGT                ACTGG(1/-1)
BstF5I        140  CATCC                GGATG(2/0)
BstUI          20  CGCG                 CG^CG
CfoI          281  GCGC                 GCG^C
DraII         565  AGGGCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      775  GACCTTCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          597  CTCTTC               CTCTTC(1/4)
EcoO109I      565  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       790  CCATGG               C^C[AT][AT]GG
FokI          140  CATCC                GGATG(9/13)
HaeII         280  AGCGCC               [GA]GCGC^[CT]
HhaI          281  GCGC                 GCG^C
HinP1I        281  GCGC                 G^CGC
HindIII       364  AAGCTT               A^AGCTT
HinfI         641  GACTC                G^A[ACGT]TC
KpnI          657  GGTACC               GGTAC^C
Ksp632I       597  CTCTTC               CTCTTC(1/4)
MseI          835  TTAA                 T^TAA
MspA1I        309  CAGCTG               C[AC]G^C[GT]G
MvnI           20  CGCG                 CG^CG
NcoI          790  CCATGG               C^CATGG
NruI           19  TCGCGA               TCG^CGA
PflFI         758  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         824  ACCGGT               A^CCGGT
PleI          641  GACTC                GAGTC(4/5)
PstI          636  CTGCAG               CTGCA^G
PvuII         309  CAGCTG               CAG^CTG
RcaI          845  TCATGA               T^CATGA
SexAI         193  ACCTGGT              A^CC[AT]GGT
SfcI          636  CTGCAG               C^T[GA][CT]AG
SgrAI         823  CACCGGTG             C[GA]^CCGG[CT]G
SmaI          213  CCCGGG               CCC^GGG
StyI          790  CCATGG               C^C[AT][AT]GG
Tru9I         835  TTAA                 T^TAA
Tth111I       758  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         213  CCCGGG               C^CCGGG
XmaI          213  CCCGGG               C^CCGGG
XmnI          314  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BfaI            7  CTAG                 C^TAG
              895  CTAG                
BsmFI          59  GTCCC                GGGAC(10/14)
              783  GTCCC               
BsrFI         200  GCCGGT               [GA]^CCGG[CT]
              824  ACCGGT              
BstXI         607  CCATCTTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              661  CCATTGTCATGG        
BstYI         125  AGATCT               [GA]^GATC[CT]
              620  GGATCC              
Cfr10I        200  GCCGGT               [GA]^CCGG[CT]
              824  ACCGGT              
DsaI          376  CCACGG               C^C[GA][CT]GG
              790  CCATGG              
EcoRI         516  GAATTC               G^AATTC
              874  GAATTC              
FauI          143  CCCGC                CCCGC(4/6)
              647  CCCGC               
MaeI            7  CTAG                 C^TAG
              895  CTAG                
MflI          125  AGATCT               [GA]^GATC[CT]
              620  GGATCC              
MslI          662  CATTGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              760  CACTGTCATG          
SmlI          129  CTTGAG               C^T[CT][GA]AG
              261  CTCAAG              
TspRI         760  CACTG                CA[GC]TG[ACGT][ACGT]^
              913  CACTG               
XcmI           75  CCAAATTTGGATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              680  CCACCGTGACTGTGG     
XhoII         125  AGATCT               [GA]^GATC[CT]
              620  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BanI        BbvI        BcnI        BmyI        
BsaJI       BsaWI       BsiYI       BslI        BsmAI       
Bsp1286I    BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      HaeIII      
HapII       HgaI        HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
TseI        Tsp45I      Tsp509I