################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BfrI BglII BlnI BsaAI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 2340 GTCTAC GT^[AC][GT]AC AcyI 727 GACGCC G[GA]^CG[CT]C AflIII 1567 ACATGT A^C[GA][CT]GT AlwNI 279 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1276 GGGCCC GGGCC^C Asp700I 1910 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 360 GAGCAC G[AT]GC[AT]^C BalI 1726 TGGCCA TGG^CCA BamHI 553 GGATCC G^GATCC BbsI 2646 GTCTTC GAAGAC(2/6) BbvCI 117 GCTGAGG CCTCAGC(-5/-2) BcgI 2312 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1812 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 95 GCTGAGC GC^T[ACGT]AGC BpmI 148 CTGGAG CTGGAG(16/14) Bpu1102I 95 GCTGAGC GC^T[ACGT]AGC BpuAI 2646 GTCTTC GAAGAC(2/6) BsaHI 727 GACGCC G[GA]^CG[CT]C BsaI 61 GGTCTC GGTCTC(1/5) BsaWI 1776 TCCGGT [AT]^CCGG[AT] BsgI 1404 GTGCAG GTGCAG(16/14) BsiHKAI 360 GAGCAC G[AT]GC[AT]^C BspHI 2011 TCATGA T^CATGA BspLU11I 1567 ACATGT A^CATGT BsrBI 1332 GAGCGG CCGCTC(-3/-3) BstBI 2515 TTCGAA TT^CGAA BstXI 543 CCATCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 95 GCTGAGC GC^T[ACGT]AGC EaeI 1726 TGGCCA [CT]^GGCC[GA] EcoRV 626 GATATC GAT^ATC HgaI 727 GACGC GACGC(5/10) Hin1I 727 GACGCC G[GA]^CG[CT]C HindIII 1839 AAGCTT A^AGCTT MluNI 1726 TGGCCA TGG^CCA MscI 1726 TGGCCA TGG^CCA MspA1I 115 CAGCTG C[AC]G^C[GT]G NspV 2515 TTCGAA TT^CGAA PaeR7I 2242 CTCGAG C^TCGAG PflMI 1007 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1460 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 115 CAGCTG CAG^CTG RcaI 2011 TCATGA T^CATGA SapI 408 GAAGAGC GCTCTTC(1/4) SfuI 2515 TTCGAA TT^CGAA SmaI 178 CCCGGG CCC^GGG StuI 398 AGGCCT AGG^CCT Van91I 1007 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2126 TCTAGA T^CTAGA XhoI 2242 CTCGAG C^TCGAG XmaCI 178 CCCGGG C^CCGGG XmaI 178 CCCGGG C^CCGGG XmnI 1910 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AocI 1203 CCTCAGG CC^T[ACGT]AGG 1919 CCTCAGG BanI 507 GGTGCC G^G[CT][GA]CC 2001 GGCACC BciVI 1599 GGATAC GTATCC(6/5) 1452 GTATCC BclI 229 TGATCA T^GATCA 620 TGATCA Bsu36I 1203 CCTCAGG CC^T[ACGT]AGG 1919 CCTCAGG CfoI 242 GCGC GCG^C 1337 GCGC EarI 408 GAAGAG CTCTTC(1/4) 678 GAAGAG Eco81I 1203 CCTCAGG CC^T[ACGT]AGG 1919 CCTCAGG FbaI 229 TGATCA T^GATCA 620 TGATCA HhaI 242 GCGC GCG^C 1337 GCGC HinP1I 242 GCGC G^CGC 1337 GCGC Ksp632I 408 GAAGAG CTCTTC(1/4) 678 GAAGAG MaeII 1084 ACGT A^CGT 2344 ACGT NdeI 1234 CATATG CA^TATG 2625 CATATG NspI 1567 ACATGT [GA]CATG^[CT] 2044 GCATGT PpuMI 744 AGGTCCT [GA]G^G[AT]CC[CT] 1252 AGGACCT SfcI 1038 CTACAG C^T[GA][CT]AG 2329 CTACAG XcmI 916 CCATCATTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1729 CCAAGGATGTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaI AvaII BanII BbvI BcnI BfaI BmyI BsaBI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII