##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BfrI BglII BlnI BsaAI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FseI FspI
HaeII HincII HindII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 2340 GTCTAC GT^[AC][GT]AC
AcyI 727 GACGCC G[GA]^CG[CT]C
AflIII 1567 ACATGT A^C[GA][CT]GT
AlwNI 279 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1276 GGGCCC GGGCC^C
Asp700I 1910 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 360 GAGCAC G[AT]GC[AT]^C
BalI 1726 TGGCCA TGG^CCA
BamHI 553 GGATCC G^GATCC
BbsI 2646 GTCTTC GAAGAC(2/6)
BbvCI 117 GCTGAGG CCTCAGC(-5/-2)
BcgI 2312 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1812 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 95 GCTGAGC GC^T[ACGT]AGC
BpmI 148 CTGGAG CTGGAG(16/14)
Bpu1102I 95 GCTGAGC GC^T[ACGT]AGC
BpuAI 2646 GTCTTC GAAGAC(2/6)
BsaHI 727 GACGCC G[GA]^CG[CT]C
BsaI 61 GGTCTC GGTCTC(1/5)
BsaWI 1776 TCCGGT [AT]^CCGG[AT]
BsgI 1404 GTGCAG GTGCAG(16/14)
BsiHKAI 360 GAGCAC G[AT]GC[AT]^C
BspHI 2011 TCATGA T^CATGA
BspLU11I 1567 ACATGT A^CATGT
BsrBI 1332 GAGCGG CCGCTC(-3/-3)
BstBI 2515 TTCGAA TT^CGAA
BstXI 543 CCATCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 95 GCTGAGC GC^T[ACGT]AGC
EaeI 1726 TGGCCA [CT]^GGCC[GA]
EcoRV 626 GATATC GAT^ATC
HgaI 727 GACGC GACGC(5/10)
Hin1I 727 GACGCC G[GA]^CG[CT]C
HindIII 1839 AAGCTT A^AGCTT
MluNI 1726 TGGCCA TGG^CCA
MscI 1726 TGGCCA TGG^CCA
MspA1I 115 CAGCTG C[AC]G^C[GT]G
NspV 2515 TTCGAA TT^CGAA
PaeR7I 2242 CTCGAG C^TCGAG
PflMI 1007 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1460 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 115 CAGCTG CAG^CTG
RcaI 2011 TCATGA T^CATGA
SapI 408 GAAGAGC GCTCTTC(1/4)
SfuI 2515 TTCGAA TT^CGAA
SmaI 178 CCCGGG CCC^GGG
StuI 398 AGGCCT AGG^CCT
Van91I 1007 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2126 TCTAGA T^CTAGA
XhoI 2242 CTCGAG C^TCGAG
XmaCI 178 CCCGGG C^CCGGG
XmaI 178 CCCGGG C^CCGGG
XmnI 1910 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AocI 1203 CCTCAGG CC^T[ACGT]AGG
1919 CCTCAGG
BanI 507 GGTGCC G^G[CT][GA]CC
2001 GGCACC
BciVI 1599 GGATAC GTATCC(6/5)
1452 GTATCC
BclI 229 TGATCA T^GATCA
620 TGATCA
Bsu36I 1203 CCTCAGG CC^T[ACGT]AGG
1919 CCTCAGG
CfoI 242 GCGC GCG^C
1337 GCGC
EarI 408 GAAGAG CTCTTC(1/4)
678 GAAGAG
Eco81I 1203 CCTCAGG CC^T[ACGT]AGG
1919 CCTCAGG
FbaI 229 TGATCA T^GATCA
620 TGATCA
HhaI 242 GCGC GCG^C
1337 GCGC
HinP1I 242 GCGC G^CGC
1337 GCGC
Ksp632I 408 GAAGAG CTCTTC(1/4)
678 GAAGAG
MaeII 1084 ACGT A^CGT
2344 ACGT
NdeI 1234 CATATG CA^TATG
2625 CATATG
NspI 1567 ACATGT [GA]CATG^[CT]
2044 GCATGT
PpuMI 744 AGGTCCT [GA]G^G[AT]CC[CT]
1252 AGGACCT
SfcI 1038 CTACAG C^T[GA][CT]AG
2329 CTACAG
XcmI 916 CCATCATTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1729 CCAAGGATGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BsaBI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII