##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbvCI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsmBI BsmFI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcsI 77 GAATTC [GA]^AATT[CT]
AgeI 581 ACCGGT A^CCGGT
Alw44I 799 GTGCAC G^TGCAC
AlwNI 719 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 257 CCTCAGG CC^T[ACGT]AGG
ApaLI 799 GTGCAC G^TGCAC
ApoI 77 GAATTC [GA]^AATT[CT]
BclI 280 TGATCA T^GATCA
BcnI 709 CCGGG CC^[GC]GG
BfaI 162 CTAG C^TAG
BpmI 632 CTCCAG CTGGAG(16/14)
BsaJI 15 CCCTGG C^C[ACGT][ACGT]GG
BseRI 68 GAGGAG GAGGAG(10/8)
Bsp1407I 105 TGTACA T^GTACA
BsrBI 463 CCGCTC CCGCTC(-3/-3)
BsrGI 105 TGTACA T^GTACA
BssSI 1088 CACGAG CACGAG(-5/-1)
Bsu36I 257 CCTCAGG CC^T[ACGT]AGG
CfoI 551 GCGC GCG^C
EarI 1193 GAAGAG CTCTTC(1/4)
Eco81I 257 CCTCAGG CC^T[ACGT]AGG
EcoNI 16 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 77 GAATTC G^AATTC
FauI 547 GCGGG CCCGC(4/6)
FbaI 280 TGATCA T^GATCA
HgaI 655 GCGTC GACGC(5/10)
HhaI 551 GCGC GCG^C
HinP1I 551 GCGC G^CGC
Ksp632I 1193 GAAGAG CTCTTC(1/4)
MaeI 162 CTAG C^TAG
MaeII 35 ACGT A^CGT
MseI 243 TTAA T^TAA
NciI 709 CCGGG CC^[GC]GG
NdeI 369 CATATG CA^TATG
NlaIII 1166 CATG CATG^
PinAI 581 ACCGGT A^CCGGT
PleI 158 GAGTC GAGTC(4/5)
SfcI 130 CTATAG C^T[GA][CT]AG
SmlI 484 CTTGAG C^T[CT][GA]AG
SspBI 105 TGTACA T^GTACA
TaqI 895 TCGA T^CGA
Tru9I 243 TTAA T^TAA
TthHB8I 895 TCGA T^CGA
XbaI 161 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AspHI 629 GTGCTC G[AT]GC[AT]^C
799 GTGCAC
AvaII 85 GGACC G^G[AT]CC
712 GGTCC
BamHI 489 GGATCC G^GATCC
1413 GGATCC
BcgI 721 GCAGCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1057 GCAGCTTGTTCG
BmyI 629 GTGCTC G[AGT]GC[ACT]^C
799 GTGCAC
BsaWI 581 ACCGGT [AT]^CCGG[AT]
1323 ACCGGA
BsiHKAI 629 GTGCTC G[AT]GC[AT]^C
799 GTGCAC
BsiWI 678 CGTACG C^GTACG
1182 CGTACG
Bsp1286I 629 GTGCTC G[AGT]GC[ACT]^C
799 GTGCAC
Cac8I 547 GCGGGC GC[ACGT]^[ACGT]GC
724 GCTGGC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
BbsI BbvI BpuAI BsaI BsiYI
BslI BsmAI BsmI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cfr10I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SgrAI StuI TfiI TseI Tsp45I
Tsp509I TspRI XhoII XmnI