################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsmBI BsmFI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 77 GAATTC [GA]^AATT[CT] AgeI 581 ACCGGT A^CCGGT Alw44I 799 GTGCAC G^TGCAC AlwNI 719 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 257 CCTCAGG CC^T[ACGT]AGG ApaLI 799 GTGCAC G^TGCAC ApoI 77 GAATTC [GA]^AATT[CT] BclI 280 TGATCA T^GATCA BcnI 709 CCGGG CC^[GC]GG BfaI 162 CTAG C^TAG BpmI 632 CTCCAG CTGGAG(16/14) BsaJI 15 CCCTGG C^C[ACGT][ACGT]GG BseRI 68 GAGGAG GAGGAG(10/8) Bsp1407I 105 TGTACA T^GTACA BsrBI 463 CCGCTC CCGCTC(-3/-3) BsrGI 105 TGTACA T^GTACA BssSI 1088 CACGAG CACGAG(-5/-1) Bsu36I 257 CCTCAGG CC^T[ACGT]AGG CfoI 551 GCGC GCG^C EarI 1193 GAAGAG CTCTTC(1/4) Eco81I 257 CCTCAGG CC^T[ACGT]AGG EcoNI 16 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 77 GAATTC G^AATTC FauI 547 GCGGG CCCGC(4/6) FbaI 280 TGATCA T^GATCA HgaI 655 GCGTC GACGC(5/10) HhaI 551 GCGC GCG^C HinP1I 551 GCGC G^CGC Ksp632I 1193 GAAGAG CTCTTC(1/4) MaeI 162 CTAG C^TAG MaeII 35 ACGT A^CGT MseI 243 TTAA T^TAA NciI 709 CCGGG CC^[GC]GG NdeI 369 CATATG CA^TATG NlaIII 1166 CATG CATG^ PinAI 581 ACCGGT A^CCGGT PleI 158 GAGTC GAGTC(4/5) SfcI 130 CTATAG C^T[GA][CT]AG SmlI 484 CTTGAG C^T[CT][GA]AG SspBI 105 TGTACA T^GTACA TaqI 895 TCGA T^CGA Tru9I 243 TTAA T^TAA TthHB8I 895 TCGA T^CGA XbaI 161 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AspHI 629 GTGCTC G[AT]GC[AT]^C 799 GTGCAC AvaII 85 GGACC G^G[AT]CC 712 GGTCC BamHI 489 GGATCC G^GATCC 1413 GGATCC BcgI 721 GCAGCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1057 GCAGCTTGTTCG BmyI 629 GTGCTC G[AGT]GC[ACT]^C 799 GTGCAC BsaWI 581 ACCGGT [AT]^CCGG[AT] 1323 ACCGGA BsiHKAI 629 GTGCTC G[AT]GC[AT]^C 799 GTGCAC BsiWI 678 CGTACG C^GTACG 1182 CGTACG Bsp1286I 629 GTGCTC G[AGT]GC[ACT]^C 799 GTGCAC Cac8I 547 GCGGGC GC[ACGT]^[ACGT]GC 724 GCTGGC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AlwI Asp700I BbsI BbvI BpuAI BsaI BsiYI BslI BsmAI BsmI BsrFI BsrI BssKI BstF5I BstNI BstYI Cfr10I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SgrAI StuI TfiI TseI Tsp45I Tsp509I TspRI XhoII XmnI