##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfaI BglI
BlnI BpmI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflII 1068 CTTAAG C^TTAAG
AflIII 467 ACATGT A^C[GA][CT]GT
AlwNI 624 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 108 GGGCCC GGGCC^C
BbvI 96 GCAGC GCAGC(8/12)
BclI 358 TGATCA T^GATCA
BfrI 1068 CTTAAG C^TTAAG
BglII 1235 AGATCT A^GATCT
BlpI 1079 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1079 GCTGAGC GC^T[ACGT]AGC
BsaBI 542 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 176 GAGACG CGTCTC(1/5)
BsmFI 194 GTCCC GGGAC(10/14)
BspHI 355 TCATGA T^CATGA
BspLU11I 467 ACATGT A^CATGT
BspMI 65 GCAGGT ACCTGC(4/8)
BssSI 781 CTCGTG CACGAG(-5/-1)
CelII 1079 GCTGAGC GC^T[ACGT]AGC
DrdI 1232 GACAGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 522 ATGCAT ATGCA^T
FbaI 358 TGATCA T^GATCA
HgaI 853 GACGC GACGC(5/10)
HincII 333 GTCAAC GT[CT]^[GA]AC
HindII 333 GTCAAC GT[CT]^[GA]AC
MamI 542 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1069 TTAA T^TAA
MslI 407 CACATGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 694 CAGCTG C[AC]G^C[GT]G
NcoI 1169 CCATGG C^CATGG
NsiI 522 ATGCAT ATGCA^T
PvuII 694 CAGCTG CAG^CTG
RcaI 355 TCATGA T^CATGA
SexAI 511 ACCAGGT A^CC[AT]GGT
SmlI 1068 CTTAAG C^T[CT][GA]AG
SphI 304 GCATGC GCATG^C
Tru9I 1069 TTAA T^TAA
TseI 96 GCAGC G^C[AT]GC
Tsp509I 1316 AATT ^AATT
XcmI 1214 CCAGATCCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 1217 GATCC GGATC(4/5)
541 GGATC
BanII 108 GGGCCC G[GA]GC[CT]^C
1180 GGGCTC
BcnI 106 CCGGG CC^[GC]GG
686 CCCGG
BmyI 108 GGGCCC G[AGT]GC[ACT]^C
1180 GGGCTC
BseRI 119 CTCCTC GAGGAG(10/8)
939 CTCCTC
BsmAI 176 GAGAC GTCTC(1/5)
471 GTCTC
Bsp1286I 108 GGGCCC G[AGT]GC[ACT]^C
1180 GGGCTC
BsrDI 364 CATTGC GCAATG(2/0)
1075 CATTGC
CfoI 302 GCGC GCG^C
662 GCGC
DsaI 205 CCACGG C^C[GA][CT]GG
1169 CCATGG
EcoT14I 553 CCAAGG C^C[AT][AT]GG
1169 CCATGG
Fnu4HI 96 GCAGC GC^[ACGT]GC
1106 GCCGC
HhaI 302 GCGC GCG^C
662 GCGC
HinP1I 302 GCGC G^CGC
662 GCGC
HinfI 192 GAGTC G^A[ACGT]TC
482 GACTC
ItaI 96 GCAGC GC^[ACGT]GC
1106 GCCGC
MaeII 179 ACGT A^CGT
823 ACGT
NciI 106 CCGGG CC^[GC]GG
686 CCCGG
PleI 482 GACTC GAGTC(4/5)
192 GAGTC
SapI 1163 GAAGAGC GCTCTTC(1/4)
257 GCTCTTC
ScaI 454 AGTACT AGT^ACT
1011 AGTACT
StyI 553 CCAAGG C^C[AT][AT]GG
1169 CCATGG
Tsp45I 72 GTCAC ^GT[GC]AC
825 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AvaII BalI
BbsI BpuAI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII FokI HaeIII HapII
HpaII HphI Ksp632I MaeIII MboI
MboII MflI MluNI MnlI MscI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI TspRI XhoII