##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AcyI AgeI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BamHI BbeI BclI BglI BglII
BlnI BsaBI BsaHI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 860 CGCG CG^CG
AccIII 269 TCCGGA T^CCGGA
AflII 874 CTTAAG C^TTAAG
AflIII 2318 ACGTGT A^C[GA][CT]GT
AhdI 3072 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2656 GTGCAC G^TGCAC
ApaLI 2656 GTGCAC G^TGCAC
AspEI 3072 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1920 TGGCCA TGG^CCA
BanI 2364 GGCACC G^G[CT][GA]CC
BbrPI 2317 CACGTG CAC^GTG
BcgI 393 CGAGAAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2839 GTATCC GTATCC(6/5)
BcnI 3155 CCGGG CC^[GC]GG
BfrI 874 CTTAAG C^TTAAG
BsaWI 269 TCCGGA [AT]^CCGG[AT]
BseAI 269 TCCGGA T^CCGGA
BsgI 1049 CTGCAC GTGCAG(16/14)
BspDI 4 ATCGAT AT^CGAT
BspEI 269 TCCGGA T^CCGGA
BspHI 1535 TCATGA T^CATGA
BsrBI 285 CCGCTC CCGCTC(-3/-3)
BsrFI 1113 GCCGGC [GA]^CCGG[CT]
BstUI 860 CGCG CG^CG
BstXI 419 CCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 3181 GCGC GCG^C
Cfr10I 1113 GCCGGC [GA]^CCGG[CT]
ClaI 4 ATCGAT AT^CGAT
DrdI 1539 GACTATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3072 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2637 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 688 GAATTC G^AATTC
EcoT22I 304 ATGCAT ATGCA^T
FauI 947 CCCGC CCCGC(4/6)
HhaI 3181 GCGC GCG^C
HinP1I 3181 GCGC G^CGC
MluNI 1920 TGGCCA TGG^CCA
MroI 269 TCCGGA T^CCGGA
MscI 1920 TGGCCA TGG^CCA
MvnI 860 CGCG CG^CG
NaeI 1113 GCCGGC GCC^GGC
NciI 3155 CCGGG CC^[GC]GG
NgoMIV 1113 GCCGGC G^CCGGC
NsiI 304 ATGCAT ATGCA^T
PmaCI 2317 CACGTG CAC^GTG
PmlI 2317 CACGTG CAC^GTG
RcaI 1535 TCATGA T^CATGA
SacI 368 GAGCTC GAGCT^C
SpeI 162 ACTAGT A^CTAGT
XbaI 1635 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 732 GTCTAC GT^[AC][GT]AC
1629 GTCTAC
AclI 321 AACGTT AA^CGTT
2026 AACGTT
AocI 1578 CCTGAGG CC^T[ACGT]AGG
2086 CCTCAGG
BsaAI 2317 CACGTG [CT]AC^GT[GA]
2618 CACGTA
BsaI 3043 GAGACC GGTCTC(1/5)
2799 GGTCTC
BseRI 1451 CTCCTC GAGGAG(10/8)
1119 GAGGAG
BsmI 82 GAATGC GAATGC(1/-1)
205 GCATTC
BstAPI 836 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1405 GCACTTCCTGC
Bsu36I 1578 CCTGAGG CC^T[ACGT]AGG
2086 CCTCAGG
EaeI 1920 TGGCCA [CT]^GGCC[GA]
2918 CGGCCA
Eco81I 1578 CCTGAGG CC^T[ACGT]AGG
2086 CCTCAGG
HindIII 871 AAGCTT A^AGCTT
1726 AAGCTT
PshAI 1977 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3238 GACTAAGGTC
Psp1406I 321 AACGTT AA^CGTT
2026 AACGTT
SphI 1109 GCATGC GCATG^C
3014 GCATGC
TfiI 454 GATTC G^A[AT]TC
813 GAATC
XcmI 419 CCATGGAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1970 CCAAAAAGACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvCI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII