##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI Aor51HI ApaI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmBI
BsoBI BspDI BspHI BspMI BsrBI
BsrFI BssHII Bst1107I BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MscI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 925 CGCG CG^CG
AccIII 893 TCCGGA T^CCGGA
AflIII 1086 ACATGT A^C[GA][CT]GT
AhdI 1738 GACCATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1122 GTGCAC G^TGCAC
AocI 1749 CCTTAGG CC^T[ACGT]AGG
ApaLI 1122 GTGCAC G^TGCAC
AspEI 1738 GACCATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1122 GTGCAC G[AT]GC[AT]^C
AvaII 412 GGTCC G^G[AT]CC
BanI 749 GGCACC G^G[CT][GA]CC
BcnI 1773 CCGGG CC^[GC]GG
BlpI 583 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 583 GCTCAGC GC^T[ACGT]AGC
BsaI 1362 GAGACC GGTCTC(1/5)
BsaJI 1773 CCGGGG C^C[ACGT][ACGT]GG
BseAI 893 TCCGGA T^CCGGA
BseRI 391 GAGGAG GAGGAG(10/8)
BsiHKAI 1122 GTGCAC G[AT]GC[AT]^C
BsmFI 1746 GTCCC GGGAC(10/14)
BsmI 1036 GAATGC GAATGC(1/-1)
BspEI 893 TCCGGA T^CCGGA
BspLU11I 1086 ACATGT A^CATGT
BsrDI 996 CATTGC GCAATG(2/0)
BssSI 185 CTCGTG CACGAG(-5/-1)
BstAPI 980 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1313 TTCGAA TT^CGAA
BstUI 925 CGCG CG^CG
Bsu36I 1749 CCTTAGG CC^T[ACGT]AGG
Cac8I 154 GCTTGC GC[ACGT]^[ACGT]GC
CelII 583 GCTCAGC GC^T[ACGT]AGC
CfoI 924 GCGC GCG^C
Eam1105I 1738 GACCATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1749 CCTTAGG CC^T[ACGT]AGG
EcoNI 974 CCTATGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 897 GACGC GACGC(5/10)
HhaI 924 GCGC GCG^C
HinP1I 924 GCGC G^CGC
HindIII 1643 AAGCTT A^AGCTT
MroI 893 TCCGGA T^CCGGA
MseI 1667 TTAA T^TAA
MvnI 925 CGCG CG^CG
NciI 1773 CCGGG CC^[GC]GG
NdeI 1619 CATATG CA^TATG
NspV 1313 TTCGAA TT^CGAA
PflMI 1182 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 411 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 868 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 80 CTGCAG CTGCA^G
SfuI 1313 TTCGAA TT^CGAA
TaqI 1314 TCGA T^CGA
Tru9I 1667 TTAA T^TAA
TthHB8I 1314 TCGA T^CGA
Van91I 1182 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 624 CCACCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BamHI 1657 GGATCC G^GATCC
1682 GGATCC
BbsI 1584 GAAGAC GAAGAC(2/6)
1855 GTCTTC
BmyI 20 GTGCCC G[AGT]GC[ACT]^C
1122 GTGCAC
BpuAI 1584 GAAGAC GAAGAC(2/6)
1855 GTCTTC
BsaWI 893 TCCGGA [AT]^CCGG[AT]
1676 ACCGGA
BsgI 1418 CTGCAC GTGCAG(16/14)
786 GTGCAG
Bsp1286I 20 GTGCCC G[AGT]GC[ACT]^C
1122 GTGCAC
Bsp1407I 281 TGTACA T^GTACA
1671 TGTACA
BsrGI 281 TGTACA T^GTACA
1671 TGTACA
BstXI 627 CCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1041 CCAGGACTGTGG
EarI 107 CTCTTC CTCTTC(1/4)
373 GAAGAG
FauI 347 CCCGC CCCGC(4/6)
1533 GCGGG
Ksp632I 107 CTCTTC CTCTTC(1/4)
373 GAAGAG
MspA1I 630 CAGCTG C[AC]G^C[GT]G
792 CAGCTG
NspI 920 ACATGC [GA]CATG^[CT]
1086 ACATGT
PvuII 630 CAGCTG CAG^CTG
792 CAGCTG
SfcI 80 CTGCAG C^T[GA][CT]AG
870 CTGTAG
SspBI 281 TGTACA T^GTACA
1671 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I BbvI BfaI
BpmI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraII DraIII EcoO109I
EcoRI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI Tsp45I Tsp509I TspRI XhoII
XmnI