##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII Aor51HI ApaI AscI AseI
AsnI Asp718I AvaI AviII AvrII
BamHI BbrPI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI ClaI CpoI
DraI DrdI DsaI EagI EarI
EclXI Eco47III Eco52I EcoO65I EcoT14I
EcoT22I FbaI FseI FspI HpaI
KpnI Ksp632I KspI MamI MluI
MroI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 1829 GTATAC GT^[AC][GT]AC
AcyI 312 GGCGCC G[GA]^CG[CT]C
AgeI 1759 ACCGGT A^CCGGT
AhdI 1001 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1122 GATCC GGATC(4/5)
AocI 664 CCTCAGG CC^T[ACGT]AGG
Asp700I 546 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1001 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 295 TGGCCA TGG^CCA
BbeI 312 GGCGCC GGCGC^C
BcgI 1108 GCACAGTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2084 CTAG C^TAG
BpmI 6 CTCCAG CTGGAG(16/14)
BsaHI 312 GGCGCC G[GA]^CG[CT]C
BsaWI 1759 ACCGGT [AT]^CCGG[AT]
BseRI 1199 CTCCTC GAGGAG(10/8)
BsmI 1692 GAATGC GAATGC(1/-1)
BsrFI 1759 ACCGGT [GA]^CCGG[CT]
Bst1107I 1829 GTATAC GTA^TAC
BstAPI 784 GCACAGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1121 AGATCC [GA]^GATC[CT]
BstZ17I 1829 GTATAC GTA^TAC
Bsu36I 664 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1759 ACCGGT [GA]^CCGG[CT]
DraIII 1895 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 295 TGGCCA [CT]^GGCC[GA]
Eam1105I 1001 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 664 CCTCAGG CC^T[ACGT]AGG
EcoNI 2027 CCTTCATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1611 GAATTC G^AATTC
EcoRV 1433 GATATC GAT^ATC
FauI 471 CCCGC CCCGC(4/6)
HaeII 312 GGCGCC [GA]GCGC^[CT]
Hin1I 312 GGCGCC G[GA]^CG[CT]C
HincII 966 GTCAAC GT[CT]^[GA]AC
HindII 966 GTCAAC GT[CT]^[GA]AC
HindIII 1674 AAGCTT A^AGCTT
KasI 312 GGCGCC G^GCGCC
MaeI 2084 CTAG C^TAG
MfeI 104 CAATTG C^AATTG
MflI 1121 AGATCC [GA]^GATC[CT]
MluNI 295 TGGCCA TGG^CCA
MscI 295 TGGCCA TGG^CCA
MseI 1156 TTAA T^TAA
MspA1I 1037 CAGCTG C[AC]G^C[GT]G
MunI 104 CAATTG C^AATTG
NarI 312 GGCGCC GG^CGCC
PflMI 1220 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1759 ACCGGT A^CCGGT
PvuII 1037 CAGCTG CAG^CTG
SacI 1647 GAGCTC GAGCT^C
SexAI 1097 ACCTGGT A^CC[AT]GGT
SfoI 312 GGCGCC GGC^GCC
Tru9I 1156 TTAA T^TAA
Van91I 1220 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1121 AGATCC [GA]^GATC[CT]
XmnI 546 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 472 CCGC CCGC(-3/-1)
1148 GCGG
AfaI 340 GTAC GT^AC
1641 GTAC
Alw44I 1106 GTGCAC G^TGCAC
1863 GTGCAC
ApaLI 1106 GTGCAC G^TGCAC
1863 GTGCAC
BanI 312 GGCGCC G^G[CT][GA]CC
893 GGTGCC
BbvCI 1875 CCTCAGC CCTCAGC(-5/-2)
2064 CCTCAGC
BlpI 48 GCTGAGC GC^T[ACGT]AGC
390 GCTGAGC
Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC
390 GCTGAGC
BspLU11I 1777 ACATGT A^CATGT
1950 ACATGT
BsrDI 1349 CATTGC GCAATG(2/0)
1994 CATTGC
CelII 48 GCTGAGC GC^T[ACGT]AGC
390 GCTGAGC
DpnII 1122 GATC ^GATC
1970 GATC
MboI 1122 GATC ^GATC
1970 GATC
NdeII 1122 GATC ^GATC
1970 GATC
PstI 971 CTGCAG CTGCA^G
1311 CTGCAG
RsaI 340 GTAC GT^AC
1641 GTAC
Sau3AI 1122 GATC ^GATC
1970 GATC
SmlI 1482 CTCAAG C^T[CT][GA]AG
1704 CTCAAG
XcmI 1417 CCAGAGCAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1898 CCAGTGCAAACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AcsI AflIII AluI AlwNI ApoI
AspHI AspI AvaII BanII BbsI
BbvI BcnI BmyI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboII MnlI
MslI MspI MvaI MwoI NciI
NlaIII NlaIV NspI PflFI PleI
PpuMI Sau96I ScrFI SfaNI SfcI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I