################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII Aor51HI ApaI AscI AseI AsnI Asp718I AvaI AviII AvrII BamHI BbrPI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI ClaI CpoI DraI DrdI DsaI EagI EarI EclXI Eco47III Eco52I EcoO65I EcoT14I EcoT22I FbaI FseI FspI HpaI KpnI Ksp632I KspI MamI MluI MroI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 1829 GTATAC GT^[AC][GT]AC AcyI 312 GGCGCC G[GA]^CG[CT]C AgeI 1759 ACCGGT A^CCGGT AhdI 1001 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1122 GATCC GGATC(4/5) AocI 664 CCTCAGG CC^T[ACGT]AGG Asp700I 546 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1001 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 295 TGGCCA TGG^CCA BbeI 312 GGCGCC GGCGC^C BcgI 1108 GCACAGTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 2084 CTAG C^TAG BpmI 6 CTCCAG CTGGAG(16/14) BsaHI 312 GGCGCC G[GA]^CG[CT]C BsaWI 1759 ACCGGT [AT]^CCGG[AT] BseRI 1199 CTCCTC GAGGAG(10/8) BsmI 1692 GAATGC GAATGC(1/-1) BsrFI 1759 ACCGGT [GA]^CCGG[CT] Bst1107I 1829 GTATAC GTA^TAC BstAPI 784 GCACAGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1121 AGATCC [GA]^GATC[CT] BstZ17I 1829 GTATAC GTA^TAC Bsu36I 664 CCTCAGG CC^T[ACGT]AGG Cfr10I 1759 ACCGGT [GA]^CCGG[CT] DraIII 1895 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 295 TGGCCA [CT]^GGCC[GA] Eam1105I 1001 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 664 CCTCAGG CC^T[ACGT]AGG EcoNI 2027 CCTTCATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1611 GAATTC G^AATTC EcoRV 1433 GATATC GAT^ATC FauI 471 CCCGC CCCGC(4/6) HaeII 312 GGCGCC [GA]GCGC^[CT] Hin1I 312 GGCGCC G[GA]^CG[CT]C HincII 966 GTCAAC GT[CT]^[GA]AC HindII 966 GTCAAC GT[CT]^[GA]AC HindIII 1674 AAGCTT A^AGCTT KasI 312 GGCGCC G^GCGCC MaeI 2084 CTAG C^TAG MfeI 104 CAATTG C^AATTG MflI 1121 AGATCC [GA]^GATC[CT] MluNI 295 TGGCCA TGG^CCA MscI 295 TGGCCA TGG^CCA MseI 1156 TTAA T^TAA MspA1I 1037 CAGCTG C[AC]G^C[GT]G MunI 104 CAATTG C^AATTG NarI 312 GGCGCC GG^CGCC PflMI 1220 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1759 ACCGGT A^CCGGT PvuII 1037 CAGCTG CAG^CTG SacI 1647 GAGCTC GAGCT^C SexAI 1097 ACCTGGT A^CC[AT]GGT SfoI 312 GGCGCC GGC^GCC Tru9I 1156 TTAA T^TAA Van91I 1220 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1121 AGATCC [GA]^GATC[CT] XmnI 546 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 472 CCGC CCGC(-3/-1) 1148 GCGG AfaI 340 GTAC GT^AC 1641 GTAC Alw44I 1106 GTGCAC G^TGCAC 1863 GTGCAC ApaLI 1106 GTGCAC G^TGCAC 1863 GTGCAC BanI 312 GGCGCC G^G[CT][GA]CC 893 GGTGCC BbvCI 1875 CCTCAGC CCTCAGC(-5/-2) 2064 CCTCAGC BlpI 48 GCTGAGC GC^T[ACGT]AGC 390 GCTGAGC Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC 390 GCTGAGC BspLU11I 1777 ACATGT A^CATGT 1950 ACATGT BsrDI 1349 CATTGC GCAATG(2/0) 1994 CATTGC CelII 48 GCTGAGC GC^T[ACGT]AGC 390 GCTGAGC DpnII 1122 GATC ^GATC 1970 GATC MboI 1122 GATC ^GATC 1970 GATC NdeII 1122 GATC ^GATC 1970 GATC PstI 971 CTGCAG CTGCA^G 1311 CTGCAG RsaI 340 GTAC GT^AC 1641 GTAC Sau3AI 1122 GATC ^GATC 1970 GATC SmlI 1482 CTCAAG C^T[CT][GA]AG 1704 CTCAAG XcmI 1417 CCAGAGCAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1898 CCAGTGCAAACATGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AcsI AflIII AluI AlwNI ApoI AspHI AspI AvaII BanII BbsI BbvI BcnI BmyI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MslI MspI MvaI MwoI NciI NlaIII NlaIV NspI PflFI PleI PpuMI Sau96I ScrFI SfaNI SfcI TaqI TfiI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I