##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BciVI BclI BcnI BfrI BlnI
BsaAI BsaI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FspI HincII HindII HindIII
HpaI KasI KspI MluI MspA1I
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 795 GACGTC GACGT^C
Acc65I 76 GGTACC G^GTACC
AccII 36 CGCG CG^CG
AccIII 814 TCCGGA T^CCGGA
AcyI 795 GACGTC G[GA]^CG[CT]C
AlwNI 720 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1279 GGGCCC GGGCC^C
Asp700I 135 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 76 GGTACC G^GTACC
AspHI 1050 GTGCTC G[AT]GC[AT]^C
AvaII 14 GGACC G^G[AT]CC
BalI 244 TGGCCA TGG^CCA
BamHI 1222 GGATCC G^GATCC
BanI 76 GGTACC G^G[CT][GA]CC
BbvCI 515 GCTGAGG CCTCAGC(-5/-2)
BglI 246 GCCACAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 596 AGATCT A^GATCT
BlpI 1206 GCTAAGC GC^T[ACGT]AGC
BpmI 831 CTGGAG CTGGAG(16/14)
Bpu1102I 1206 GCTAAGC GC^T[ACGT]AGC
BsaBI 584 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 795 GACGTC G[GA]^CG[CT]C
BseAI 814 TCCGGA T^CCGGA
BsiHKAI 1050 GTGCTC G[AT]GC[AT]^C
BspEI 814 TCCGGA T^CCGGA
BsrFI 740 GCCGGC [GA]^CCGG[CT]
BstAPI 719 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 36 CGCG CG^CG
BstXI 355 CCAAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1206 GCTAAGC GC^T[ACGT]AGC
CfoI 22 GCGC GCG^C
Cfr10I 740 GCCGGC [GA]^CCGG[CT]
DraII 1278 GGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 244 TGGCCA [CT]^GGCC[GA]
EarI 927 GAAGAG CTCTTC(1/4)
EcoNI 1269 CCTTGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1278 GGGGCCC [GA]G^G[ACGT]CC[CT]
FseI 739 GGCCGGCC GGCCGG^CC
HaeII 21 AGCGCC [GA]GCGC^[CT]
HgaI 456 GCGTC GACGC(5/10)
HhaI 22 GCGC GCG^C
Hin1I 795 GACGTC G[GA]^CG[CT]C
HinP1I 22 GCGC G^CGC
KpnI 76 GGTACC GGTAC^C
Ksp632I 927 GAAGAG CTCTTC(1/4)
MaeII 796 ACGT A^CGT
MamI 584 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 229 CAATTG C^AATTG
MluNI 244 TGGCCA TGG^CCA
MroI 814 TCCGGA T^CCGGA
MscI 244 TGGCCA TGG^CCA
MslI 53 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 229 CAATTG C^AATTG
MvnI 36 CGCG CG^CG
NaeI 740 GCCGGC GCC^GGC
NgoMIV 740 GCCGGC G^CCGGC
PshAI 1018 GACGGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI 905 GATGC GCATC(5/9)
TaqI 1217 TCGA T^CGA
TfiI 993 GAATC G^A[AT]TC
Tsp45I 1252 GTGAC ^GT[GC]AC
TthHB8I 1217 TCGA T^CGA
XmnI 135 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 101 GAATTT [GA]^AATT[CT]
399 AAATTC
AfaI 77 GTAC GT^AC
1313 GTAC
ApoI 101 GAATTT [GA]^AATT[CT]
399 AAATTC
BcgI 1042 CGATGGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1072 GCAAACGGATCG
BsaWI 814 TCCGGA [AT]^CCGG[AT]
1232 ACCGGA
BsmFI 13 GGGAC GGGAC(10/14)
1320 GGGAC
BspMI 628 ACCTGC ACCTGC(4/8)
778 ACCTGC
BstYI 596 AGATCT [GA]^GATC[CT]
1222 GGATCC
MaeIII 1252 GTGAC ^GT[ACGT]AC
1341 GTAAC
MflI 596 AGATCT [GA]^GATC[CT]
1222 GGATCC
PflMI 236 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
854 CCACAGGATGG
RsaI 77 GTAC GT^AC
1313 GTAC
SmlI 843 CTTGAG C^T[CT][GA]AG
942 CTCAAG
Van91I 236 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
854 CCACAGGATGG
XbaI 551 TCTAGA T^CTAGA
803 TCTAGA
XhoII 596 AGATCT [GA]^GATC[CT]
1222 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfcI
StyI Tru9I TseI Tsp509I TspRI