##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Alw44I AocI ApaI ApaLI
AscI Asp718I AviII AvrII BalI
BbeI BcgI BciVI BclI BfrI
BglII BlnI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco52I
Eco81I EcoRV EcoT22I FbaI FseI
FspI HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NarI NdeI
NotI NruI NsiI PacI PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SacII SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Van91I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1018 GACGTC GACGT^C
AccI 334 GTCGAC GT^[AC][GT]AC
AcyI 1018 GACGTC G[GA]^CG[CT]C
AflIII 236 ACGTGT A^C[GA][CT]GT
AhdI 377 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2383 ATTAAT AT^TAAT
AsnI 2383 ATTAAT AT^TAAT
Asp700I 1368 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 377 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2410 CACGTG CAC^GTG
BcnI 231 CCGGG CC^[GC]GG
BfaI 496 CTAG C^TAG
BlpI 108 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 108 GCTGAGC GC^T[ACGT]AGC
BsaBI 2187 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1018 GACGTC G[GA]^CG[CT]C
BsaI 2167 GAGACC GGTCTC(1/5)
BspMI 819 GCAGGT ACCTGC(4/8)
BsrBI 111 GAGCGG CCGCTC(-3/-3)
BstAPI 948 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2442 TTCGAA TT^CGAA
BstEII 1146 GGTGACC G^GT[ACGT]ACC
BstPI 1146 GGTGACC G^GT[ACGT]ACC
CelII 108 GCTGAGC GC^T[ACGT]AGC
EaeI 1739 TGGCCG [CT]^GGCC[GA]
Eam1105I 377 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2478 CCTGCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1146 GGTGACC G^GT[ACGT]ACC
EcoRI 1054 GAATTC G^AATTC
Hin1I 1018 GACGTC G[GA]^CG[CT]C
HincII 334 GTCGAC GT[CT]^[GA]AC
HindII 334 GTCGAC GT[CT]^[GA]AC
HindIII 670 AAGCTT A^AGCTT
MaeI 496 CTAG C^TAG
MamI 2187 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1925 GCCGGC GCC^GGC
NciI 231 CCGGG CC^[GC]GG
NgoMIV 1925 GCCGGC G^CCGGC
NheI 495 GCTAGC G^CTAGC
NspV 2442 TTCGAA TT^CGAA
PmaCI 2410 CACGTG CAC^GTG
PmlI 2410 CACGTG CAC^GTG
PshBI 2383 ATTAAT AT^TAAT
SacI 991 GAGCTC GAGCT^C
SalI 334 GTCGAC G^TCGAC
SapI 1008 GAAGAGC GCTCTTC(1/4)
ScaI 2420 AGTACT AGT^ACT
SexAI 1810 ACCAGGT A^CC[AT]GGT
SfuI 2442 TTCGAA TT^CGAA
SphI 287 GCATGC GCATG^C
StuI 1214 AGGCCT AGG^CCT
XcmI 1172 CCATGCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1368 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 409 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1615 CAGATCCTG
Aor51HI 350 AGCGCT AGC^GCT
1065 AGCGCT
AspI 1045 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1809 GACCAGGTC
BamHI 2192 GGATCC G^GATCC
2261 GGATCC
BanII 991 GAGCTC G[GA]GC[CT]^C
1912 GAGCCC
BglI 362 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2292 GCCCATGCGGC
BsaAI 711 CACGTA [CT]AC^GT[GA]
2410 CACGTG
BsmBI 1511 CGTCTC CGTCTC(1/5)
1580 GAGACG
BspHI 527 TCATGA T^CATGA
872 TCATGA
BsrDI 131 CATTGC GCAATG(2/0)
910 GCAATG
BsrFI 764 ACCGGC [GA]^CCGG[CT]
1925 GCCGGC
BssSI 1490 CACGAG CACGAG(-5/-1)
2032 CTCGTG
Cfr10I 764 ACCGGC [GA]^CCGG[CT]
1925 GCCGGC
DraII 427 AGGCCCT [GA]G^G[ACGT]CC[CT]
1439 AGGCCCC
EarI 423 GAAGAG CTCTTC(1/4)
1008 GAAGAG
Eco47III 350 AGCGCT AGC^GCT
1065 AGCGCT
EcoO109I 427 AGGCCCT [GA]G^G[ACGT]CC[CT]
1439 AGGCCCC
HgaI 1510 GCGTC GACGC(5/10)
2144 GCGTC
Ksp632I 423 GAAGAG CTCTTC(1/4)
1008 GAAGAG
MseI 1336 TTAA T^TAA
2384 TTAA
NspI 287 GCATGC [GA]CATG^[CT]
1430 ACATGC
PaeR7I 340 CTCGAG C^TCGAG
2416 CTCGAG
PflFI 1045 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1809 GACCAGGTC
PvuII 1077 CAGCTG CAG^CTG
2015 CAGCTG
RcaI 527 TCATGA T^CATGA
872 TCATGA
Tru9I 1336 TTAA T^TAA
2384 TTAA
Tth111I 1045 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1809 GACCAGGTC
XhoI 340 CTCGAG C^TCGAG
2416 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanI
BbsI BbvCI BbvI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII