################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI Alw44I AocI ApaI ApaLI AscI Asp718I AviII AvrII BalI BbeI BcgI BciVI BclI BfrI BglII BlnI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MunI MvnI NarI NdeI NotI NruI NsiI PacI PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacII SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 1018 GACGTC GACGT^C AccI 334 GTCGAC GT^[AC][GT]AC AcyI 1018 GACGTC G[GA]^CG[CT]C AflIII 236 ACGTGT A^C[GA][CT]GT AhdI 377 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 2383 ATTAAT AT^TAAT AsnI 2383 ATTAAT AT^TAAT Asp700I 1368 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 377 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 2410 CACGTG CAC^GTG BcnI 231 CCGGG CC^[GC]GG BfaI 496 CTAG C^TAG BlpI 108 GCTGAGC GC^T[ACGT]AGC Bpu1102I 108 GCTGAGC GC^T[ACGT]AGC BsaBI 2187 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1018 GACGTC G[GA]^CG[CT]C BsaI 2167 GAGACC GGTCTC(1/5) BspMI 819 GCAGGT ACCTGC(4/8) BsrBI 111 GAGCGG CCGCTC(-3/-3) BstAPI 948 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2442 TTCGAA TT^CGAA BstEII 1146 GGTGACC G^GT[ACGT]ACC BstPI 1146 GGTGACC G^GT[ACGT]ACC CelII 108 GCTGAGC GC^T[ACGT]AGC EaeI 1739 TGGCCG [CT]^GGCC[GA] Eam1105I 377 GACTTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2478 CCTGCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1146 GGTGACC G^GT[ACGT]ACC EcoRI 1054 GAATTC G^AATTC Hin1I 1018 GACGTC G[GA]^CG[CT]C HincII 334 GTCGAC GT[CT]^[GA]AC HindII 334 GTCGAC GT[CT]^[GA]AC HindIII 670 AAGCTT A^AGCTT MaeI 496 CTAG C^TAG MamI 2187 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1925 GCCGGC GCC^GGC NciI 231 CCGGG CC^[GC]GG NgoMIV 1925 GCCGGC G^CCGGC NheI 495 GCTAGC G^CTAGC NspV 2442 TTCGAA TT^CGAA PmaCI 2410 CACGTG CAC^GTG PmlI 2410 CACGTG CAC^GTG PshBI 2383 ATTAAT AT^TAAT SacI 991 GAGCTC GAGCT^C SalI 334 GTCGAC G^TCGAC SapI 1008 GAAGAGC GCTCTTC(1/4) ScaI 2420 AGTACT AGT^ACT SexAI 1810 ACCAGGT A^CC[AT]GGT SfuI 2442 TTCGAA TT^CGAA SphI 287 GCATGC GCATG^C StuI 1214 AGGCCT AGG^CCT XcmI 1172 CCATGCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1368 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwNI 409 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 1615 CAGATCCTG Aor51HI 350 AGCGCT AGC^GCT 1065 AGCGCT AspI 1045 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1809 GACCAGGTC BamHI 2192 GGATCC G^GATCC 2261 GGATCC BanII 991 GAGCTC G[GA]GC[CT]^C 1912 GAGCCC BglI 362 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2292 GCCCATGCGGC BsaAI 711 CACGTA [CT]AC^GT[GA] 2410 CACGTG BsmBI 1511 CGTCTC CGTCTC(1/5) 1580 GAGACG BspHI 527 TCATGA T^CATGA 872 TCATGA BsrDI 131 CATTGC GCAATG(2/0) 910 GCAATG BsrFI 764 ACCGGC [GA]^CCGG[CT] 1925 GCCGGC BssSI 1490 CACGAG CACGAG(-5/-1) 2032 CTCGTG Cfr10I 764 ACCGGC [GA]^CCGG[CT] 1925 GCCGGC DraII 427 AGGCCCT [GA]G^G[ACGT]CC[CT] 1439 AGGCCCC EarI 423 GAAGAG CTCTTC(1/4) 1008 GAAGAG Eco47III 350 AGCGCT AGC^GCT 1065 AGCGCT EcoO109I 427 AGGCCCT [GA]G^G[ACGT]CC[CT] 1439 AGGCCCC HgaI 1510 GCGTC GACGC(5/10) 2144 GCGTC Ksp632I 423 GAAGAG CTCTTC(1/4) 1008 GAAGAG MseI 1336 TTAA T^TAA 2384 TTAA NspI 287 GCATGC [GA]CATG^[CT] 1430 ACATGC PaeR7I 340 CTCGAG C^TCGAG 2416 CTCGAG PflFI 1045 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1809 GACCAGGTC PvuII 1077 CAGCTG CAG^CTG 2015 CAGCTG RcaI 527 TCATGA T^CATGA 872 TCATGA Tru9I 1336 TTAA T^TAA 2384 TTAA Tth111I 1045 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1809 GACCAGGTC XhoI 340 CTCGAG C^TCGAG 2416 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanI BbsI BbvCI BbvI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII