################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 188 TCCGGA T^CCGGA AcsI 184 GAATTC [GA]^AATT[CT] ApoI 184 GAATTC [GA]^AATT[CT] Asp700I 64 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 50 TGGCCA TGG^CCA BciVI 407 GTATCC GTATCC(6/5) BfaI 427 CTAG C^TAG BsaAI 808 TACGTG [CT]AC^GT[GA] BsaWI 188 TCCGGA [AT]^CCGG[AT] BseAI 188 TCCGGA T^CCGGA BsgI 847 GTGCAG GTGCAG(16/14) BspEI 188 TCCGGA T^CCGGA BsrBI 6 GAGCGG CCGCTC(-3/-3) BstAPI 354 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 19 GGTGACC G^GT[ACGT]ACC BstPI 19 GGTGACC G^GT[ACGT]ACC BstYI 741 AGATCC [GA]^GATC[CT] DsaI 1142 CCGTGG C^C[GA][CT]GG EaeI 50 TGGCCA [CT]^GGCC[GA] EarI 1174 GAAGAG CTCTTC(1/4) EcoNI 1225 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 19 GGTGACC G^GT[ACGT]ACC EcoRI 184 GAATTC G^AATTC FauI 987 GCGGG CCCGC(4/6) HgaI 564 GCGTC GACGC(5/10) HindIII 138 AAGCTT A^AGCTT Ksp632I 1174 GAAGAG CTCTTC(1/4) MaeI 427 CTAG C^TAG MaeIII 20 GTGAC ^GT[ACGT]AC MflI 741 AGATCC [GA]^GATC[CT] MluNI 50 TGGCCA TGG^CCA MroI 188 TCCGGA T^CCGGA MscI 50 TGGCCA TGG^CCA MseI 578 TTAA T^TAA MslI 447 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 177 GCATGT [GA]CATG^[CT] PflMI 870 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ScaI 803 AGTACT AGT^ACT SexAI 818 ACCAGGT A^CC[AT]GGT SmaI 897 CCCGGG CCC^GGG SmlI 481 CTCAAG C^T[CT][GA]AG TfiI 888 GATTC G^A[AT]TC Tru9I 578 TTAA T^TAA Tsp45I 20 GTGAC ^GT[GC]AC Van91I 870 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 741 AGATCC [GA]^GATC[CT] XmaCI 897 CCCGGG C^CCGGG XmaI 897 CCCGGG C^CCGGG XmnI 64 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 300 GTAC GT^AC 804 GTAC AocI 909 CCTCAGG CC^T[ACGT]AGG 1045 CCTGAGG AvaI 857 CCCGAG C^[CT]CG[GA]G 897 CCCGGG BanI 730 GGCACC G^G[CT][GA]CC 1041 GGTGCC BanII 27 GAGCCC G[GA]GC[CT]^C 131 GAGCCC BcnI 897 CCCGG CC^[GC]GG 898 CCGGG BmyI 27 GAGCCC G[AGT]GC[ACT]^C 131 GAGCCC BseRI 864 CTCCTC GAGGAG(10/8) 40 GAGGAG BsoBI 857 CCCGAG C^[CT]CG[GA]G 897 CCCGGG Bsp1286I 27 GAGCCC G[AGT]GC[ACT]^C 131 GAGCCC BsrI 48 ACTGG ACTGG(1/-1) 671 ACTGG Bsu36I 909 CCTCAGG CC^T[ACGT]AGG 1045 CCTGAGG DraII 356 AGGACCT [GA]G^G[ACGT]CC[CT] 1148 AGGTCCT Eco81I 909 CCTCAGG CC^T[ACGT]AGG 1045 CCTGAGG EcoO109I 356 AGGACCT [GA]G^G[ACGT]CC[CT] 1148 AGGTCCT HapII 189 CCGG C^CGG 898 CCGG HpaII 189 CCGG C^CGG 898 CCGG MspI 189 CCGG C^CGG 898 CCGG NciI 897 CCCGG CC^[GC]GG 898 CCGGG PleI 473 GACTC GAGTC(4/5) 14 GAGTC PpuMI 356 AGGACCT [GA]G^G[AT]CC[CT] 1148 AGGTCCT PvuII 219 CAGCTG CAG^CTG 709 CAGCTG RsaI 300 GTAC GT^AC 804 GTAC SfaNI 322 GATGC GCATC(5/9) 1079 GCATC StuI 509 AGGCCT AGG^CCT 1233 AGGCCT TaqI 120 TCGA T^CGA 1097 TCGA TthHB8I 120 TCGA T^CGA 1097 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BbsI BbvI BpuAI BsaI BsaJI BsiYI BslI BsmAI BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraIII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfcI TseI Tsp509I TspRI