##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 188 TCCGGA T^CCGGA
AcsI 184 GAATTC [GA]^AATT[CT]
ApoI 184 GAATTC [GA]^AATT[CT]
Asp700I 64 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 50 TGGCCA TGG^CCA
BciVI 407 GTATCC GTATCC(6/5)
BfaI 427 CTAG C^TAG
BsaAI 808 TACGTG [CT]AC^GT[GA]
BsaWI 188 TCCGGA [AT]^CCGG[AT]
BseAI 188 TCCGGA T^CCGGA
BsgI 847 GTGCAG GTGCAG(16/14)
BspEI 188 TCCGGA T^CCGGA
BsrBI 6 GAGCGG CCGCTC(-3/-3)
BstAPI 354 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 19 GGTGACC G^GT[ACGT]ACC
BstPI 19 GGTGACC G^GT[ACGT]ACC
BstYI 741 AGATCC [GA]^GATC[CT]
DsaI 1142 CCGTGG C^C[GA][CT]GG
EaeI 50 TGGCCA [CT]^GGCC[GA]
EarI 1174 GAAGAG CTCTTC(1/4)
EcoNI 1225 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 19 GGTGACC G^GT[ACGT]ACC
EcoRI 184 GAATTC G^AATTC
FauI 987 GCGGG CCCGC(4/6)
HgaI 564 GCGTC GACGC(5/10)
HindIII 138 AAGCTT A^AGCTT
Ksp632I 1174 GAAGAG CTCTTC(1/4)
MaeI 427 CTAG C^TAG
MaeIII 20 GTGAC ^GT[ACGT]AC
MflI 741 AGATCC [GA]^GATC[CT]
MluNI 50 TGGCCA TGG^CCA
MroI 188 TCCGGA T^CCGGA
MscI 50 TGGCCA TGG^CCA
MseI 578 TTAA T^TAA
MslI 447 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 177 GCATGT [GA]CATG^[CT]
PflMI 870 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 803 AGTACT AGT^ACT
SexAI 818 ACCAGGT A^CC[AT]GGT
SmaI 897 CCCGGG CCC^GGG
SmlI 481 CTCAAG C^T[CT][GA]AG
TfiI 888 GATTC G^A[AT]TC
Tru9I 578 TTAA T^TAA
Tsp45I 20 GTGAC ^GT[GC]AC
Van91I 870 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 741 AGATCC [GA]^GATC[CT]
XmaCI 897 CCCGGG C^CCGGG
XmaI 897 CCCGGG C^CCGGG
XmnI 64 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 300 GTAC GT^AC
804 GTAC
AocI 909 CCTCAGG CC^T[ACGT]AGG
1045 CCTGAGG
AvaI 857 CCCGAG C^[CT]CG[GA]G
897 CCCGGG
BanI 730 GGCACC G^G[CT][GA]CC
1041 GGTGCC
BanII 27 GAGCCC G[GA]GC[CT]^C
131 GAGCCC
BcnI 897 CCCGG CC^[GC]GG
898 CCGGG
BmyI 27 GAGCCC G[AGT]GC[ACT]^C
131 GAGCCC
BseRI 864 CTCCTC GAGGAG(10/8)
40 GAGGAG
BsoBI 857 CCCGAG C^[CT]CG[GA]G
897 CCCGGG
Bsp1286I 27 GAGCCC G[AGT]GC[ACT]^C
131 GAGCCC
BsrI 48 ACTGG ACTGG(1/-1)
671 ACTGG
Bsu36I 909 CCTCAGG CC^T[ACGT]AGG
1045 CCTGAGG
DraII 356 AGGACCT [GA]G^G[ACGT]CC[CT]
1148 AGGTCCT
Eco81I 909 CCTCAGG CC^T[ACGT]AGG
1045 CCTGAGG
EcoO109I 356 AGGACCT [GA]G^G[ACGT]CC[CT]
1148 AGGTCCT
HapII 189 CCGG C^CGG
898 CCGG
HpaII 189 CCGG C^CGG
898 CCGG
MspI 189 CCGG C^CGG
898 CCGG
NciI 897 CCCGG CC^[GC]GG
898 CCGGG
PleI 473 GACTC GAGTC(4/5)
14 GAGTC
PpuMI 356 AGGACCT [GA]G^G[AT]CC[CT]
1148 AGGTCCT
PvuII 219 CAGCTG CAG^CTG
709 CAGCTG
RsaI 300 GTAC GT^AC
804 GTAC
SfaNI 322 GATGC GCATC(5/9)
1079 GCATC
StuI 509 AGGCCT AGG^CCT
1233 AGGCCT
TaqI 120 TCGA T^CGA
1097 TCGA
TthHB8I 120 TCGA T^CGA
1097 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbsI BbvI BpuAI BsaI BsaJI
BsiYI BslI BsmAI BspMI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraIII EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeII
MboI MboII MnlI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp509I TspRI