##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        Alw44I      
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AviII       AvrII       BalI        BanI        
BbeI        BciVI       BclI        BglI        BglII       
BlnI        BsaBI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspLU11I    BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     CpoI        DraI        DrdI        EagI        
EclXI       Eco52I      EcoO65I     EcoRI       EcoT22I     
FbaI        FseI        FspI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MscI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    RsrII       SacII       
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Van91I      XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII        345  TCCGGA               T^CCGGA
AflII        1501  CTTAAG               C^TTAAG
AflIII       1443  ACGTGT               A^C[GA][CT]GT
AhdI          107  GACGCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I       760  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         107  GACGCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1436  GAGCTC               G[AT]GC[AT]^C
AspI         1295  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        1048  GCTGAGG              CCTCAGC(-5/-2)
BcgI          906  CGATCGACGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         1501  CTTAAG               C^TTAAG
BsaI         1328  GGTCTC               GGTCTC(1/5)
BsaWI         345  TCCGGA               [AT]^CCGG[AT]
BseAI         345  TCCGGA               T^CCGGA
BsiEI         906  CGATCG               CG[GA][CT]^CG
BsiHKAI      1436  GAGCTC               G[AT]GC[AT]^C
BspDI         386  ATCGAT               AT^CGAT
BspEI         345  TCCGGA               T^CCGGA
BspHI          72  TCATGA               T^CATGA
BsrDI         651  CATTGC               GCAATG(2/0)
BstAPI        646  GCAGTCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI          386  ATCGAT               AT^CGAT
DraIII        524  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      107  GACGCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1198  CCTGTCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         791  GATATC               GAT^ATC
HindIII      2213  AAGCTT               A^AGCTT
HpaI         2445  GTTAAC               GTT^AAC
MroI          345  TCCGGA               T^CCGGA
NaeI         1402  GCCGGC               GCC^GGC
NgoMIV       1402  GCCGGC               G^CCGGC
PflFI        1295  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuI          906  CGATCG               CGAT^CG
RcaI           72  TCATGA               T^CATGA
SacI         1436  GAGCTC               GAGCT^C
SalI         1466  GTCGAC               G^TCGAC
TfiI         1506  GAATC                G^A[AT]TC
Tth111I      1295  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1489  TCTAGA               T^CTAGA
XcmI          507  CCAGCTCGAGGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          760  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI          254  GAATTT               [GA]^AATT[CT]
             1520  AAATTT              
AcyI          107  GACGCC               G[GA]^CG[CT]C
              635  GACGCC              
ApoI          254  GAATTT               [GA]^AATT[CT]
             1520  AAATTT              
BamHI         348  GGATCC               G^GATCC
             1689  GGATCC              
BbrPI         776  CACGTG               CAC^GTG
             1442  CACGTG              
BbsI          149  GTCTTC               GAAGAC(2/6)
              237  GTCTTC              
BpuAI         149  GTCTTC               GAAGAC(2/6)
              237  GTCTTC              
BsaAI         776  CACGTG               [CT]AC^GT[GA]
             1442  CACGTG              
BsaHI         107  GACGCC               G[GA]^CG[CT]C
              635  GACGCC              
BsrFI         366  ACCGGC               [GA]^CCGG[CT]
             1402  GCCGGC              
BssSI        1868  CACGAG               CACGAG(-5/-1)
             1277  CTCGTG              
Cfr10I        366  ACCGGC               [GA]^CCGG[CT]
             1402  GCCGGC              
EaeI          195  TGGCCG               [CT]^GGCC[GA]
             1152  TGGCCG              
Hin1I         107  GACGCC               G[GA]^CG[CT]C
              635  GACGCC              
MslI          997  CATTCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2176  CATTGAGATG          
PaeR7I        511  CTCGAG               C^TCGAG
             2463  CTCGAG              
PmaCI         776  CACGTG               CAC^GTG
             1442  CACGTG              
PmlI          776  CACGTG               CAC^GTG
             1442  CACGTG              
PvuII        1610  CAGCTG               CAG^CTG
             1816  CAGCTG              
XhoI          511  CTCGAG               C^TCGAG
             2463  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AccI        AccII       AciI        AfaI        AluI        
AlwI        AlwNI       AocI        Aor51HI     AvaI        
AvaII       BanII       BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
Eco47III    Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        RsaI        SapI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII