################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AclI AgeI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AviII AvrII BalI BanI BbeI BciVI BclI BglI BglII BlnI BsaBI BsiWI BsmBI BsmI Bsp1407I BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CpoI DraI DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmeI PshAI PshBI Psp1406I RsrII SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 345 TCCGGA T^CCGGA AflII 1501 CTTAAG C^TTAAG AflIII 1443 ACGTGT A^C[GA][CT]GT AhdI 107 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 760 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 107 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1436 GAGCTC G[AT]GC[AT]^C AspI 1295 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 1048 GCTGAGG CCTCAGC(-5/-2) BcgI 906 CGATCGACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1501 CTTAAG C^TTAAG BsaI 1328 GGTCTC GGTCTC(1/5) BsaWI 345 TCCGGA [AT]^CCGG[AT] BseAI 345 TCCGGA T^CCGGA BsiEI 906 CGATCG CG[GA][CT]^CG BsiHKAI 1436 GAGCTC G[AT]GC[AT]^C BspDI 386 ATCGAT AT^CGAT BspEI 345 TCCGGA T^CCGGA BspHI 72 TCATGA T^CATGA BsrDI 651 CATTGC GCAATG(2/0) BstAPI 646 GCAGTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 386 ATCGAT AT^CGAT DraIII 524 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 107 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1198 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 791 GATATC GAT^ATC HindIII 2213 AAGCTT A^AGCTT HpaI 2445 GTTAAC GTT^AAC MroI 345 TCCGGA T^CCGGA NaeI 1402 GCCGGC GCC^GGC NgoMIV 1402 GCCGGC G^CCGGC PflFI 1295 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 906 CGATCG CGAT^CG RcaI 72 TCATGA T^CATGA SacI 1436 GAGCTC GAGCT^C SalI 1466 GTCGAC G^TCGAC TfiI 1506 GAATC G^A[AT]TC Tth111I 1295 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1489 TCTAGA T^CTAGA XcmI 507 CCAGCTCGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 760 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 254 GAATTT [GA]^AATT[CT] 1520 AAATTT AcyI 107 GACGCC G[GA]^CG[CT]C 635 GACGCC ApoI 254 GAATTT [GA]^AATT[CT] 1520 AAATTT BamHI 348 GGATCC G^GATCC 1689 GGATCC BbrPI 776 CACGTG CAC^GTG 1442 CACGTG BbsI 149 GTCTTC GAAGAC(2/6) 237 GTCTTC BpuAI 149 GTCTTC GAAGAC(2/6) 237 GTCTTC BsaAI 776 CACGTG [CT]AC^GT[GA] 1442 CACGTG BsaHI 107 GACGCC G[GA]^CG[CT]C 635 GACGCC BsrFI 366 ACCGGC [GA]^CCGG[CT] 1402 GCCGGC BssSI 1868 CACGAG CACGAG(-5/-1) 1277 CTCGTG Cfr10I 366 ACCGGC [GA]^CCGG[CT] 1402 GCCGGC EaeI 195 TGGCCG [CT]^GGCC[GA] 1152 TGGCCG Hin1I 107 GACGCC G[GA]^CG[CT]C 635 GACGCC MslI 997 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2176 CATTGAGATG PaeR7I 511 CTCGAG C^TCGAG 2463 CTCGAG PmaCI 776 CACGTG CAC^GTG 1442 CACGTG PmlI 776 CACGTG CAC^GTG 1442 CACGTG PvuII 1610 CAGCTG CAG^CTG 1816 CAGCTG XhoI 511 CTCGAG C^TCGAG 2463 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AlwNI AocI Aor51HI AvaI AvaII BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco47III Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII