##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AviII AvrII BalI BanI
BbeI BciVI BclI BglI BglII
BlnI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CpoI DraI DrdI EagI
EclXI Eco52I EcoO65I EcoRI EcoT22I
FbaI FseI FspI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflMI PinAI PmeI
PshAI PshBI Psp1406I RsrII SacII
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 345 TCCGGA T^CCGGA
AflII 1501 CTTAAG C^TTAAG
AflIII 1443 ACGTGT A^C[GA][CT]GT
AhdI 107 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 760 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 107 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1436 GAGCTC G[AT]GC[AT]^C
AspI 1295 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1048 GCTGAGG CCTCAGC(-5/-2)
BcgI 906 CGATCGACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1501 CTTAAG C^TTAAG
BsaI 1328 GGTCTC GGTCTC(1/5)
BsaWI 345 TCCGGA [AT]^CCGG[AT]
BseAI 345 TCCGGA T^CCGGA
BsiEI 906 CGATCG CG[GA][CT]^CG
BsiHKAI 1436 GAGCTC G[AT]GC[AT]^C
BspDI 386 ATCGAT AT^CGAT
BspEI 345 TCCGGA T^CCGGA
BspHI 72 TCATGA T^CATGA
BsrDI 651 CATTGC GCAATG(2/0)
BstAPI 646 GCAGTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 386 ATCGAT AT^CGAT
DraIII 524 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 107 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1198 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 791 GATATC GAT^ATC
HindIII 2213 AAGCTT A^AGCTT
HpaI 2445 GTTAAC GTT^AAC
MroI 345 TCCGGA T^CCGGA
NaeI 1402 GCCGGC GCC^GGC
NgoMIV 1402 GCCGGC G^CCGGC
PflFI 1295 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 906 CGATCG CGAT^CG
RcaI 72 TCATGA T^CATGA
SacI 1436 GAGCTC GAGCT^C
SalI 1466 GTCGAC G^TCGAC
TfiI 1506 GAATC G^A[AT]TC
Tth111I 1295 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1489 TCTAGA T^CTAGA
XcmI 507 CCAGCTCGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 760 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 254 GAATTT [GA]^AATT[CT]
1520 AAATTT
AcyI 107 GACGCC G[GA]^CG[CT]C
635 GACGCC
ApoI 254 GAATTT [GA]^AATT[CT]
1520 AAATTT
BamHI 348 GGATCC G^GATCC
1689 GGATCC
BbrPI 776 CACGTG CAC^GTG
1442 CACGTG
BbsI 149 GTCTTC GAAGAC(2/6)
237 GTCTTC
BpuAI 149 GTCTTC GAAGAC(2/6)
237 GTCTTC
BsaAI 776 CACGTG [CT]AC^GT[GA]
1442 CACGTG
BsaHI 107 GACGCC G[GA]^CG[CT]C
635 GACGCC
BsrFI 366 ACCGGC [GA]^CCGG[CT]
1402 GCCGGC
BssSI 1868 CACGAG CACGAG(-5/-1)
1277 CTCGTG
Cfr10I 366 ACCGGC [GA]^CCGG[CT]
1402 GCCGGC
EaeI 195 TGGCCG [CT]^GGCC[GA]
1152 TGGCCG
Hin1I 107 GACGCC G[GA]^CG[CT]C
635 GACGCC
MslI 997 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2176 CATTGAGATG
PaeR7I 511 CTCGAG C^TCGAG
2463 CTCGAG
PmaCI 776 CACGTG CAC^GTG
1442 CACGTG
PmlI 776 CACGTG CAC^GTG
1442 CACGTG
PvuII 1610 CAGCTG CAG^CTG
1816 CAGCTG
XhoI 511 CTCGAG C^TCGAG
2463 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI Aor51HI AvaI
AvaII BanII BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
Eco47III Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII