##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AgeI AhdI AlwI AlwNI AocI
ApaI AscI Asp700I Asp718I AspEI
AspI AvaI AviII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HapII HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MspA1I MspI MunI MvnI
MwoI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AciI 127 CCGC CCGC(-3/-1)
AclI 952 AACGTT AA^CGTT
AcyI 571 GGCGTC G[GA]^CG[CT]C
AflII 993 CTTAAG C^TTAAG
AflIII 1826 ACATGT A^C[GA][CT]GT
Alw44I 817 GTGCAC G^TGCAC
Aor51HI 759 AGCGCT AGC^GCT
ApaLI 817 GTGCAC G^TGCAC
AseI 231 ATTAAT AT^TAAT
AsnI 231 ATTAAT AT^TAAT
AspHI 817 GTGCAC G[AT]GC[AT]^C
AvrII 642 CCTAGG C^CTAGG
BalI 7 TGGCCA TGG^CCA
BanI 1799 GGCACC G^G[CT][GA]CC
BbsI 849 GAAGAC GAAGAC(2/6)
BfrI 993 CTTAAG C^TTAAG
BlnI 642 CCTAGG C^CTAGG
BmyI 817 GTGCAC G[AGT]GC[ACT]^C
BpuAI 849 GAAGAC GAAGAC(2/6)
BsaHI 571 GGCGTC G[GA]^CG[CT]C
BsaI 1432 GAGACC GGTCTC(1/5)
BseRI 247 CTCCTC GAGGAG(10/8)
BsiHKAI 817 GTGCAC G[AT]GC[AT]^C
BsmFI 602 GTCCC GGGAC(10/14)
Bsp1286I 817 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 1169 TGTACA T^GTACA
BspLU11I 1826 ACATGT A^CATGT
BsrDI 1258 GCAATG GCAATG(2/0)
BsrGI 1169 TGTACA T^GTACA
BstBI 1293 TTCGAA TT^CGAA
CfoI 760 GCGC GCG^C
DraI 336 TTTAAA TTT^AAA
DraII 600 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 1465 CCATGG C^C[GA][CT]GG
EaeI 7 TGGCCA [CT]^GGCC[GA]
Eco47III 759 AGCGCT AGC^GCT
EcoO109I 600 GGGTCCC [GA]G^G[ACGT]CC[CT]
HaeII 759 AGCGCT [GA]GCGC^[CT]
HgaI 572 GCGTC GACGC(5/10)
HhaI 760 GCGC GCG^C
Hin1I 571 GGCGTC G[GA]^CG[CT]C
HinP1I 760 GCGC G^CGC
MluNI 7 TGGCCA TGG^CCA
MscI 7 TGGCCA TGG^CCA
MslI 1173 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1465 CCATGG C^CATGG
NdeI 1560 CATATG CA^TATG
NspV 1293 TTCGAA TT^CGAA
PpuMI 600 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 231 ATTAAT AT^TAAT
Psp1406I 952 AACGTT AA^CGTT
PstI 1343 CTGCAG CTGCA^G
SapI 192 GCTCTTC GCTCTTC(1/4)
SfuI 1293 TTCGAA TT^CGAA
SspBI 1169 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 601 GGTCC G^G[AT]CC
615 GGTCC
BglII 897 AGATCT A^GATCT
1806 AGATCT
BpmI 549 CTGGAG CTGGAG(16/14)
1485 CTGGAG
BsmI 434 GAATGC GAATGC(1/-1)
1460 GCATTC
BstYI 897 AGATCT [GA]^GATC[CT]
1806 AGATCT
Cfr13I 601 GGTCC G^G[ACGT]CC
615 GGTCC
EarI 193 CTCTTC CTCTTC(1/4)
1278 GAAGAG
EcoT14I 642 CCTAGG C^C[AT][AT]GG
1465 CCATGG
Ksp632I 193 CTCTTC CTCTTC(1/4)
1278 GAAGAG
MaeII 484 ACGT A^CGT
953 ACGT
MflI 897 AGATCT [GA]^GATC[CT]
1806 AGATCT
Sau96I 601 GGTCC G^G[ACGT]CC
615 GGTCC
StyI 642 CCTAGG C^C[AT][AT]GG
1465 CCATGG
TaqI 71 TCGA T^CGA
1294 TCGA
TfiI 1007 GAATC G^A[AT]TC
1546 GAATC
TthHB8I 71 TCGA T^CGA
1294 TCGA
XhoII 897 AGATCT [GA]^GATC[CT]
1806 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
ScrFI SfaNI SfcI SmlI SphI
Tru9I TseI Tsp45I Tsp509I TspRI