##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI Asp718I AspEI AspI AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRV FbaI
FseI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcsI 589 GAATTC [GA]^AATT[CT]
AfaI 1678 GTAC GT^AC
AlwNI 1255 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 220 AGCGCT AGC^GCT
ApoI 589 GAATTC [GA]^AATT[CT]
AseI 1014 ATTAAT AT^TAAT
AsnI 1014 ATTAAT AT^TAAT
AvaI 352 CCCGGG C^[CT]CG[GA]G
AviII 1918 TGCGCA TGC^GCA
BamHI 1749 GGATCC G^GATCC
BglI 1709 GCCATGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 613 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 613 GCTGAGC GC^T[ACGT]AGC
BsaI 1147 GAGACC GGTCTC(1/5)
BsmFI 399 GTCCC GGGAC(10/14)
BsoBI 352 CCCGGG C^[CT]CG[GA]G
BspHI 944 TCATGA T^CATGA
BsrFI 99 GCCGGC [GA]^CCGG[CT]
BssSI 330 CACGAG CACGAG(-5/-1)
BstXI 2021 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 613 GCTGAGC GC^T[ACGT]AGC
Cfr10I 99 GCCGGC [GA]^CCGG[CT]
DraI 645 TTTAAA TTT^AAA
Eco47III 220 AGCGCT AGC^GCT
EcoRI 589 GAATTC G^AATTC
EcoT22I 1005 ATGCAT ATGCA^T
FspI 1918 TGCGCA TGC^GCA
HaeII 220 AGCGCT [GA]GCGC^[CT]
NaeI 99 GCCGGC GCC^GGC
NdeI 1772 CATATG CA^TATG
NgoMIV 99 GCCGGC G^CCGGC
NsiI 1005 ATGCAT ATGCA^T
PflMI 1619 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 171 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1014 ATTAAT AT^TAAT
PstI 337 CTGCAG CTGCA^G
RcaI 944 TCATGA T^CATGA
RsaI 1678 GTAC GT^AC
SapI 1096 GAAGAGC GCTCTTC(1/4)
SmaI 352 CCCGGG CCC^GGG
SspI 2230 AATATT AAT^ATT
Van91I 1619 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 808 CCAAATGCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 352 CCCGGG C^CCGGG
XmaI 352 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 78 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
877 GAAAACCTTC
BbsI 865 GAAGAC GAAGAC(2/6)
273 GTCTTC
BpuAI 865 GAAGAC GAAGAC(2/6)
273 GTCTTC
BsaAI 391 TACGTG [CT]AC^GT[GA]
2035 TACGTG
BspMI 699 ACCTGC ACCTGC(4/8)
2242 GCAGGT
BsrDI 1791 CATTGC GCAATG(2/0)
1327 GCAATG
BstYI 822 GGATCT [GA]^GATC[CT]
1749 GGATCC
CfoI 221 GCGC GCG^C
1919 GCGC
DraII 171 GGGTCCT [GA]G^G[ACGT]CC[CT]
1686 AGGGCCT
EcoNI 132 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
402 CCTAGAGGAGG
EcoO109I 171 GGGTCCT [GA]G^G[ACGT]CC[CT]
1686 AGGGCCT
FauI 373 CCCGC CCCGC(4/6)
118 GCGGG
HhaI 221 GCGC GCG^C
1919 GCGC
HinP1I 221 GCGC G^CGC
1919 GCGC
HincII 528 GTTGAC GT[CT]^[GA]AC
1647 GTCAAC
HindII 528 GTTGAC GT[CT]^[GA]AC
1647 GTCAAC
MaeII 392 ACGT A^CGT
2036 ACGT
MflI 822 GGATCT [GA]^GATC[CT]
1749 GGATCC
MslI 1654 CATAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1922 CACCGATGTG
SacI 469 GAGCTC GAGCT^C
1099 GAGCTC
SfcI 337 CTGCAG C^T[GA][CT]AG
1397 CTACAG
SmlI 914 CTCAAG C^T[CT][GA]AG
998 CTCAAG
XhoII 822 GGATCT [GA]^GATC[CT]
1749 GGATCC
XmnI 78 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
877 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BalI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PvuII Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I