################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AspI AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV FbaI FseI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 589 GAATTC [GA]^AATT[CT] AfaI 1678 GTAC GT^AC AlwNI 1255 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 220 AGCGCT AGC^GCT ApoI 589 GAATTC [GA]^AATT[CT] AseI 1014 ATTAAT AT^TAAT AsnI 1014 ATTAAT AT^TAAT AvaI 352 CCCGGG C^[CT]CG[GA]G AviII 1918 TGCGCA TGC^GCA BamHI 1749 GGATCC G^GATCC BglI 1709 GCCATGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 613 GCTGAGC GC^T[ACGT]AGC Bpu1102I 613 GCTGAGC GC^T[ACGT]AGC BsaI 1147 GAGACC GGTCTC(1/5) BsmFI 399 GTCCC GGGAC(10/14) BsoBI 352 CCCGGG C^[CT]CG[GA]G BspHI 944 TCATGA T^CATGA BsrFI 99 GCCGGC [GA]^CCGG[CT] BssSI 330 CACGAG CACGAG(-5/-1) BstXI 2021 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 613 GCTGAGC GC^T[ACGT]AGC Cfr10I 99 GCCGGC [GA]^CCGG[CT] DraI 645 TTTAAA TTT^AAA Eco47III 220 AGCGCT AGC^GCT EcoRI 589 GAATTC G^AATTC EcoT22I 1005 ATGCAT ATGCA^T FspI 1918 TGCGCA TGC^GCA HaeII 220 AGCGCT [GA]GCGC^[CT] NaeI 99 GCCGGC GCC^GGC NdeI 1772 CATATG CA^TATG NgoMIV 99 GCCGGC G^CCGGC NsiI 1005 ATGCAT ATGCA^T PflMI 1619 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 171 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 1014 ATTAAT AT^TAAT PstI 337 CTGCAG CTGCA^G RcaI 944 TCATGA T^CATGA RsaI 1678 GTAC GT^AC SapI 1096 GAAGAGC GCTCTTC(1/4) SmaI 352 CCCGGG CCC^GGG SspI 2230 AATATT AAT^ATT Van91I 1619 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 808 CCAAATGCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 352 CCCGGG C^CCGGG XmaI 352 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Asp700I 78 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 877 GAAAACCTTC BbsI 865 GAAGAC GAAGAC(2/6) 273 GTCTTC BpuAI 865 GAAGAC GAAGAC(2/6) 273 GTCTTC BsaAI 391 TACGTG [CT]AC^GT[GA] 2035 TACGTG BspMI 699 ACCTGC ACCTGC(4/8) 2242 GCAGGT BsrDI 1791 CATTGC GCAATG(2/0) 1327 GCAATG BstYI 822 GGATCT [GA]^GATC[CT] 1749 GGATCC CfoI 221 GCGC GCG^C 1919 GCGC DraII 171 GGGTCCT [GA]G^G[ACGT]CC[CT] 1686 AGGGCCT EcoNI 132 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 402 CCTAGAGGAGG EcoO109I 171 GGGTCCT [GA]G^G[ACGT]CC[CT] 1686 AGGGCCT FauI 373 CCCGC CCCGC(4/6) 118 GCGGG HhaI 221 GCGC GCG^C 1919 GCGC HinP1I 221 GCGC G^CGC 1919 GCGC HincII 528 GTTGAC GT[CT]^[GA]AC 1647 GTCAAC HindII 528 GTTGAC GT[CT]^[GA]AC 1647 GTCAAC MaeII 392 ACGT A^CGT 2036 ACGT MflI 822 GGATCT [GA]^GATC[CT] 1749 GGATCC MslI 1654 CATAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1922 CACCGATGTG SacI 469 GAGCTC GAGCT^C 1099 GAGCTC SfcI 337 CTGCAG C^T[GA][CT]AG 1397 CTACAG SmlI 914 CTCAAG C^T[CT][GA]AG 998 CTCAAG XhoII 822 GGATCT [GA]^GATC[CT] 1749 GGATCC XmnI 78 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 877 GAAAACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AluI AlwI AspHI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PvuII Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I