################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AclI 1544 AACGTT AA^CGTT AcsI 38 GAATTC [GA]^AATT[CT] AflIII 752 ACATGT A^C[GA][CT]GT AgeI 986 ACCGGT A^CCGGT Alw44I 1563 GTGCAC G^TGCAC ApaLI 1563 GTGCAC G^TGCAC ApoI 38 GAATTC [GA]^AATT[CT] BamHI 792 GGATCC G^GATCC BanII 488 GGGCTC G[GA]GC[CT]^C BglI 395 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 204 AGATCT A^GATCT BsaBI 522 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1657 GAGACC GGTCTC(1/5) BsaWI 986 ACCGGT [AT]^CCGG[AT] BsiEI 984 CGACCG CG[GA][CT]^CG BspLU11I 752 ACATGT A^CATGT BsrDI 1051 GCAATG GCAATG(2/0) BsrFI 986 ACCGGT [GA]^CCGG[CT] BstEII 1507 GGTCACC G^GT[ACGT]ACC BstPI 1507 GGTCACC G^GT[ACGT]ACC CfoI 1279 GCGC GCG^C Cfr10I 986 ACCGGT [GA]^CCGG[CT] DraII 595 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 595 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 1507 GGTCACC G^GT[ACGT]ACC EcoRI 38 GAATTC G^AATTC EcoRV 221 GATATC GAT^ATC FauI 1605 GCGGG CCCGC(4/6) HgaI 1141 GCGTC GACGC(5/10) HhaI 1279 GCGC GCG^C HinP1I 1279 GCGC G^CGC MamI 522 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 531 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NheI 927 GCTAGC G^CTAGC PinAI 986 ACCGGT A^CCGGT PpuMI 595 AGGACCT [GA]G^G[AT]CC[CT] PshAI 318 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1544 AACGTT AA^CGTT SphI 669 GCATGC GCATG^C StuI 338 AGGCCT AGG^CCT TaqI 983 TCGA T^CGA TthHB8I 983 TCGA T^CGA XcmI 1494 CCAGCTGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 1020 CCGC CCGC(-3/-1) 1605 GCGG AfaI 740 GTAC GT^AC 1491 GTAC AlwNI 594 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 1270 CAGAAGCTG AocI 652 CCTCAGG CC^T[ACGT]AGG 836 CCTCAGG BfaI 266 CTAG C^TAG 928 CTAG BseRI 445 CTCCTC GAGGAG(10/8) 781 GAGGAG BspMI 598 ACCTGC ACCTGC(4/8) 1249 GCAGGT BstAPI 551 GCATCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1379 GCAGTGCTTGC Bsu36I 652 CCTCAGG CC^T[ACGT]AGG 836 CCTCAGG DsaI 114 CCATGG C^C[GA][CT]GG 686 CCATGG Eco81I 652 CCTCAGG CC^T[ACGT]AGG 836 CCTCAGG EcoNI 648 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 916 CCTTTCACAGG HinfI 272 GAGTC G^A[ACGT]TC 1041 GACTC MaeI 266 CTAG C^TAG 928 CTAG MaeII 773 ACGT A^CGT 1545 ACGT MseI 176 TTAA T^TAA 197 TTAA NcoI 114 CCATGG C^CATGG 686 CCATGG NspI 669 GCATGC [GA]CATG^[CT] 752 ACATGT PleI 1041 GACTC GAGTC(4/5) 272 GAGTC RsaI 740 GTAC GT^AC 1491 GTAC SmlI 517 CTCAAG C^T[CT][GA]AG 1080 CTCAAG Tru9I 176 TTAA T^TAA 197 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AluI AlwI AspHI AvaII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I Tsp509I TspRI XhoII