##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsgI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshBI PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 1544 AACGTT AA^CGTT
AcsI 38 GAATTC [GA]^AATT[CT]
AflIII 752 ACATGT A^C[GA][CT]GT
AgeI 986 ACCGGT A^CCGGT
Alw44I 1563 GTGCAC G^TGCAC
ApaLI 1563 GTGCAC G^TGCAC
ApoI 38 GAATTC [GA]^AATT[CT]
BamHI 792 GGATCC G^GATCC
BanII 488 GGGCTC G[GA]GC[CT]^C
BglI 395 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 204 AGATCT A^GATCT
BsaBI 522 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1657 GAGACC GGTCTC(1/5)
BsaWI 986 ACCGGT [AT]^CCGG[AT]
BsiEI 984 CGACCG CG[GA][CT]^CG
BspLU11I 752 ACATGT A^CATGT
BsrDI 1051 GCAATG GCAATG(2/0)
BsrFI 986 ACCGGT [GA]^CCGG[CT]
BstEII 1507 GGTCACC G^GT[ACGT]ACC
BstPI 1507 GGTCACC G^GT[ACGT]ACC
CfoI 1279 GCGC GCG^C
Cfr10I 986 ACCGGT [GA]^CCGG[CT]
DraII 595 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 595 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1507 GGTCACC G^GT[ACGT]ACC
EcoRI 38 GAATTC G^AATTC
EcoRV 221 GATATC GAT^ATC
FauI 1605 GCGGG CCCGC(4/6)
HgaI 1141 GCGTC GACGC(5/10)
HhaI 1279 GCGC GCG^C
HinP1I 1279 GCGC G^CGC
MamI 522 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 531 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 927 GCTAGC G^CTAGC
PinAI 986 ACCGGT A^CCGGT
PpuMI 595 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 318 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1544 AACGTT AA^CGTT
SphI 669 GCATGC GCATG^C
StuI 338 AGGCCT AGG^CCT
TaqI 983 TCGA T^CGA
TthHB8I 983 TCGA T^CGA
XcmI 1494 CCAGCTGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 1020 CCGC CCGC(-3/-1)
1605 GCGG
AfaI 740 GTAC GT^AC
1491 GTAC
AlwNI 594 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1270 CAGAAGCTG
AocI 652 CCTCAGG CC^T[ACGT]AGG
836 CCTCAGG
BfaI 266 CTAG C^TAG
928 CTAG
BseRI 445 CTCCTC GAGGAG(10/8)
781 GAGGAG
BspMI 598 ACCTGC ACCTGC(4/8)
1249 GCAGGT
BstAPI 551 GCATCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1379 GCAGTGCTTGC
Bsu36I 652 CCTCAGG CC^T[ACGT]AGG
836 CCTCAGG
DsaI 114 CCATGG C^C[GA][CT]GG
686 CCATGG
Eco81I 652 CCTCAGG CC^T[ACGT]AGG
836 CCTCAGG
EcoNI 648 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
916 CCTTTCACAGG
HinfI 272 GAGTC G^A[ACGT]TC
1041 GACTC
MaeI 266 CTAG C^TAG
928 CTAG
MaeII 773 ACGT A^CGT
1545 ACGT
MseI 176 TTAA T^TAA
197 TTAA
NcoI 114 CCATGG C^CATGG
686 CCATGG
NspI 669 GCATGC [GA]CATG^[CT]
752 ACATGT
PleI 1041 GACTC GAGTC(4/5)
272 GAGTC
RsaI 740 GTAC GT^AC
1491 GTAC
SmlI 517 CTCAAG C^T[CT][GA]AG
1080 CTCAAG
Tru9I 176 TTAA T^TAA
197 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AluI AlwI AspHI AvaII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I Tsp509I TspRI XhoII