##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AhdI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BseAI       
BsgI        BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoT22I     FbaI        FseI        FspI        
HaeII       Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PmaCI       
PmeI        PmlI        PshBI       PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AclI         1544  AACGTT               AA^CGTT
AcsI           38  GAATTC               [GA]^AATT[CT]
AflIII        752  ACATGT               A^C[GA][CT]GT
AgeI          986  ACCGGT               A^CCGGT
Alw44I       1563  GTGCAC               G^TGCAC
ApaLI        1563  GTGCAC               G^TGCAC
ApoI           38  GAATTC               [GA]^AATT[CT]
BamHI         792  GGATCC               G^GATCC
BanII         488  GGGCTC               G[GA]GC[CT]^C
BglI          395  GCCACAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         204  AGATCT               A^GATCT
BsaBI         522  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1657  GAGACC               GGTCTC(1/5)
BsaWI         986  ACCGGT               [AT]^CCGG[AT]
BsiEI         984  CGACCG               CG[GA][CT]^CG
BspLU11I      752  ACATGT               A^CATGT
BsrDI        1051  GCAATG               GCAATG(2/0)
BsrFI         986  ACCGGT               [GA]^CCGG[CT]
BstEII       1507  GGTCACC              G^GT[ACGT]ACC
BstPI        1507  GGTCACC              G^GT[ACGT]ACC
CfoI         1279  GCGC                 GCG^C
Cfr10I        986  ACCGGT               [GA]^CCGG[CT]
DraII         595  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      595  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1507  GGTCACC              G^GT[ACGT]ACC
EcoRI          38  GAATTC               G^AATTC
EcoRV         221  GATATC               GAT^ATC
FauI         1605  GCGGG                CCCGC(4/6)
HgaI         1141  GCGTC                GACGC(5/10)
HhaI         1279  GCGC                 GCG^C
HinP1I       1279  GCGC                 G^CGC
MamI          522  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          531  CACCCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI          927  GCTAGC               G^CTAGC
PinAI         986  ACCGGT               A^CCGGT
PpuMI         595  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         318  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1544  AACGTT               AA^CGTT
SphI          669  GCATGC               GCATG^C
StuI          338  AGGCCT               AGG^CCT
TaqI          983  TCGA                 T^CGA
TthHB8I       983  TCGA                 T^CGA
XcmI         1494  CCAGCTGCCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI         1020  CCGC                 CCGC(-3/-1)
             1605  GCGG                
AfaI          740  GTAC                 GT^AC
             1491  GTAC                
AlwNI         594  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1270  CAGAAGCTG           
AocI          652  CCTCAGG              CC^T[ACGT]AGG
              836  CCTCAGG             
BfaI          266  CTAG                 C^TAG
              928  CTAG                
BseRI         445  CTCCTC               GAGGAG(10/8)
              781  GAGGAG              
BspMI         598  ACCTGC               ACCTGC(4/8)
             1249  GCAGGT              
BstAPI        551  GCATCTCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1379  GCAGTGCTTGC         
Bsu36I        652  CCTCAGG              CC^T[ACGT]AGG
              836  CCTCAGG             
DsaI          114  CCATGG               C^C[GA][CT]GG
              686  CCATGG              
Eco81I        652  CCTCAGG              CC^T[ACGT]AGG
              836  CCTCAGG             
EcoNI         648  CCTGCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              916  CCTTTCACAGG         
HinfI         272  GAGTC                G^A[ACGT]TC
             1041  GACTC               
MaeI          266  CTAG                 C^TAG
              928  CTAG                
MaeII         773  ACGT                 A^CGT
             1545  ACGT                
MseI          176  TTAA                 T^TAA
              197  TTAA                
NcoI          114  CCATGG               C^CATGG
              686  CCATGG              
NspI          669  GCATGC               [GA]CATG^[CT]
              752  ACATGT              
PleI         1041  GACTC                GAGTC(4/5)
              272  GAGTC               
RsaI          740  GTAC                 GT^AC
             1491  GTAC                
SmlI          517  CTCAAG               C^T[CT][GA]AG
             1080  CTCAAG              
Tru9I         176  TTAA                 T^TAA
              197  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AluI        AlwI        AspHI       AvaII       BbsI        
BbvI        BcnI        BmyI        BpmI        BpuAI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TseI        Tsp45I      Tsp509I     TspRI       XhoII