##################################
# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tsp45I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 327 GTAGAC GT^[AC][GT]AC
AciI 230 CCGC CCGC(-3/-1)
AlwNI 107 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 248 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 145 GAGCCC G[GA]GC[CT]^C
BciVI 375 GTATCC GTATCC(6/5)
BmyI 145 GAGCCC G[AGT]GC[ACT]^C
Bsp1286I 145 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 114 TGTACA T^GTACA
BspMI 67 ACCTGC ACCTGC(4/8)
BsrGI 114 TGTACA T^GTACA
BsrI 458 CCAGT ACTGG(1/-1)
BstAPI 550 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 226 AGATCC [GA]^GATC[CT]
DsaI 217 CCATGG C^C[GA][CT]GG
EcoT14I 217 CCATGG C^C[AT][AT]GG
HinfI 533 GAATC G^A[ACGT]TC
HphI 186 GGTGA GGTGA(8/7)
MflI 226 AGATCC [GA]^GATC[CT]
MwoI 550 GCAGATTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 217 CCATGG C^CATGG
NheI 513 GCTAGC G^CTAGC
NspI 79 GCATGT [GA]CATG^[CT]
PvuII 465 CAGCTG CAG^CTG
SspBI 114 TGTACA T^GTACA
StyI 217 CCATGG C^C[AT][AT]GG
TaqI 256 TCGA T^CGA
TfiI 533 GAATC G^A[AT]TC
TthHB8I 256 TCGA T^CGA
XhoII 226 AGATCC [GA]^GATC[CT]
XmnI 248 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 101 GATCC GGATC(4/5)
227 GATCC
BfaI 452 CTAG C^TAG
514 CTAG
BsaJI 148 CCCTGG C^C[ACGT][ACGT]GG
217 CCATGG
BsmAI 34 GAGAC GTCTC(1/5)
83 GTCTC
BstF5I 563 CATCC GGATG(2/0)
26 GGATG
Cac8I 232 GCTGGC GC[ACGT]^[ACGT]GC
513 GCTAGC
DdeI 244 CTAAG C^T[ACGT]AG
400 CTCAG
DpnII 101 GATC ^GATC
227 GATC
EarI 18 CTCTTC CTCTTC(1/4)
85 CTCTTC
FokI 563 CATCC GGATG(9/13)
26 GGATG
Ksp632I 18 CTCTTC CTCTTC(1/4)
85 CTCTTC
MaeI 452 CTAG C^TAG
514 CTAG
MboI 101 GATC ^GATC
227 GATC
MseI 309 TTAA T^TAA
599 TTAA
MspA1I 230 CCGCTG C[AC]G^C[GT]G
465 CAGCTG
NdeII 101 GATC ^GATC
227 GATC
NlaIII 80 CATG CATG^
218 CATG
Sau3AI 101 GATC ^GATC
227 GATC
SfcI 295 CTGTAG C^T[GA][CT]AG
338 CTACAG
Tru9I 309 TTAA T^TAA
599 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsiYI
BslI BsmFI BssKI BstNI Cfr13I
EcoRII Fnu4HI HaeIII ItaI MboII
MnlI MvaI NlaIV RsaI Sau96I
ScrFI TseI TspRI