##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
AlwNI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbrPI BbsI
BclI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluNI MroI MscI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tth111I Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 224 GGCGCC G[GA]^CG[CT]C
AflIII 59 ACGCGT A^C[GA][CT]GT
Alw44I 704 GTGCAC G^TGCAC
AocI 1023 CCTGAGG CC^T[ACGT]AGG
ApaLI 704 GTGCAC G^TGCAC
AspHI 704 GTGCAC G[AT]GC[AT]^C
AviII 138 TGCGCA TGC^GCA
BbeI 224 GGCGCC GGCGC^C
BbvCI 93 GCTGAGG CCTCAGC(-5/-2)
BciVI 807 GTATCC GTATCC(6/5)
BsaHI 224 GGCGCC G[GA]^CG[CT]C
BsaWI 314 TCCGGT [AT]^CCGG[AT]
BsgI 1119 GTGCAG GTGCAG(16/14)
BsiHKAI 704 GTGCAC G[AT]GC[AT]^C
BstBI 864 TTCGAA TT^CGAA
BstXI 472 CCACAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 461 AGATCC [GA]^GATC[CT]
Bsu36I 1023 CCTGAGG CC^T[ACGT]AGG
DrdI 1073 GACTAATAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 601 TGGCCG [CT]^GGCC[GA]
Eco81I 1023 CCTGAGG CC^T[ACGT]AGG
FspI 138 TGCGCA TGC^GCA
Hin1I 224 GGCGCC G[GA]^CG[CT]C
HphI 1062 GGTGA GGTGA(8/7)
KasI 224 GGCGCC G^GCGCC
MaeII 345 ACGT A^CGT
MflI 461 AGATCC [GA]^GATC[CT]
MluI 59 ACGCGT A^CGCGT
MseI 1162 TTAA T^TAA
NarI 224 GGCGCC GG^CGCC
NruI 533 TCGCGA TCG^CGA
NspV 864 TTCGAA TT^CGAA
PstI 104 CTGCAG CTGCA^G
SfoI 224 GGCGCC GGC^GCC
SfuI 864 TTCGAA TT^CGAA
SmaI 192 CCCGGG CCC^GGG
TfiI 262 GATTC G^A[AT]TC
Tru9I 1162 TTAA T^TAA
Tsp509I 1139 AATT ^AATT
XcmI 1104 CCAGCATCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 461 AGATCC [GA]^GATC[CT]
XmaCI 192 CCCGGG C^CCGGG
XmaI 192 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Aor51HI 303 AGCGCT AGC^GCT
360 AGCGCT
BanI 224 GGCGCC G^G[CT][GA]CC
1087 GGTGCC
BanII 196 GGGCTC G[GA]GC[CT]^C
1028 GGGCTC
BcgI 7 CGAAAGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
796 GCAGAAGTCTCG
BfaI 322 CTAG C^TAG
570 CTAG
BpmI 457 CTCCAG CTGGAG(16/14)
592 CTCCAG
BsmBI 62 CGTCTC CGTCTC(1/5)
912 GAGACG
DraII 40 GGGACCC [GA]G^G[ACGT]CC[CT]
239 GGGTCCC
Eco47III 303 AGCGCT AGC^GCT
360 AGCGCT
EcoO109I 40 GGGACCC [GA]G^G[ACGT]CC[CT]
239 GGGTCCC
MaeI 322 CTAG C^TAG
570 CTAG
MspA1I 91 CAGCTG C[AC]G^C[GT]G
756 CAGCTG
NlaIII 817 CATG CATG^
826 CATG
PaeR7I 364 CTCGAG C^TCGAG
439 CTCGAG
PpuMI 40 GGGACCC [GA]G^G[AT]CC[CT]
239 GGGTCCC
PvuII 91 CAGCTG CAG^CTG
756 CAGCTG
SfcI 104 CTGCAG C^T[GA][CT]AG
1097 CTACAG
StuI 11 AGGCCT AGG^CCT
1012 AGGCCT
XhoI 364 CTCGAG C^TCGAG
439 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AluI AlwI AvaI
AvaII BbvI BcnI BlpI BmyI
Bpu1102I BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TseI Tsp45I
TspRI TthHB8I