##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI AlwI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BclI
BfrI BglI BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HpaI HphI KasI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PvuII RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 20 GACGTC GACGT^C
Acc65I 7 GGTACC G^GTACC
AccII 1398 CGCG CG^CG
AcyI 20 GACGTC G[GA]^CG[CT]C
Alw44I 1377 GTGCAC G^TGCAC
AocI 200 CCTGAGG CC^T[ACGT]AGG
ApaI 1266 GGGCCC GGGCC^C
ApaLI 1377 GTGCAC G^TGCAC
Asp718I 7 GGTACC G^GTACC
AspI 1332 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 538 CCTCAGC CCTCAGC(-5/-2)
BciVI 1354 GGATAC GTATCC(6/5)
BglII 685 AGATCT A^GATCT
BpmI 1420 CTCCAG CTGGAG(16/14)
BsaHI 20 GACGTC G[GA]^CG[CT]C
BsgI 449 GTGCAG GTGCAG(16/14)
BsiEI 577 CGATCG CG[GA][CT]^CG
BspHI 493 TCATGA T^CATGA
BssSI 1235 CACGAG CACGAG(-5/-1)
BstUI 1398 CGCG CG^CG
BstXI 1041 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 685 AGATCT [GA]^GATC[CT]
Bsu36I 200 CCTGAGG CC^T[ACGT]AGG
Eco81I 200 CCTGAGG CC^T[ACGT]AGG
EcoT22I 209 ATGCAT ATGCA^T
FauI 832 GCGGG CCCGC(4/6)
Hin1I 20 GACGTC G[GA]^CG[CT]C
HindIII 653 AAGCTT A^AGCTT
KpnI 7 GGTACC GGTAC^C
MflI 685 AGATCT [GA]^GATC[CT]
MseI 996 TTAA T^TAA
MslI 641 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1398 CGCG CG^CG
NaeI 955 GCCGGC GCC^GGC
NdeI 1136 CATATG CA^TATG
NgoMIV 955 GCCGGC G^CCGGC
NsiI 209 ATGCAT ATGCA^T
NspI 207 GCATGC [GA]CATG^[CT]
PflFI 1332 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 618 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 275 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 577 CGATCG CGAT^CG
RcaI 493 TCATGA T^CATGA
SacI 1178 GAGCTC GAGCT^C
SexAI 1161 ACCTGGT A^CC[AT]GGT
SphI 207 GCATGC GCATG^C
TaqI 364 TCGA T^CGA
Tru9I 996 TTAA T^TAA
Tsp509I 1394 AATT ^AATT
Tth111I 1332 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 364 TCGA T^CGA
XbaI 82 TCTAGA T^CTAGA
XcmI 1119 CCACAGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 685 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 1178 GAGCTC G[AT]GC[AT]^C
1377 GTGCAC
BfaI 83 CTAG C^TAG
740 CTAG
BlpI 1105 GCTGAGC GC^T[ACGT]AGC
1307 GCTGAGC
Bpu1102I 1105 GCTGAGC GC^T[ACGT]AGC
1307 GCTGAGC
BsiHKAI 1178 GAGCTC G[AT]GC[AT]^C
1377 GTGCAC
BsmBI 179 GAGACG CGTCTC(1/5)
721 GAGACG
BsrFI 955 GCCGGC [GA]^CCGG[CT]
1230 ACCGGC
CelII 1105 GCTGAGC GC^T[ACGT]AGC
1307 GCTGAGC
Cfr10I 955 GCCGGC [GA]^CCGG[CT]
1230 ACCGGC
EcoNI 189 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
790 CCTCCTACAGG
EcoT14I 332 CCTTGG C^C[AT][AT]GG
1350 CCAAGG
HaeII 374 GGCGCT [GA]GCGC^[CT]
1069 GGCGCT
HgaI 181 GACGC GACGC(5/10)
500 GACGC
HinfI 1325 GAATC G^A[ACGT]TC
1412 GAATC
MaeI 83 CTAG C^TAG
740 CTAG
MaeII 21 ACGT A^CGT
663 ACGT
MspA1I 893 CAGCGG C[AC]G^C[GT]G
1025 CAGCGG
SmlI 689 CTCAAG C^T[CT][GA]AG
1181 CTCAAG
StuI 197 AGGCCT AGG^CCT
1188 AGGCCT
StyI 332 CCTTGG C^C[AT][AT]GG
1350 CCAAGG
TfiI 1325 GAATC G^A[AT]TC
1412 GAATC
Tsp45I 273 GTGAC ^GT[GC]AC
1010 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI