################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BclI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HpaI HphI KasI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NarI NcoI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuII RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 20 GACGTC GACGT^C Acc65I 7 GGTACC G^GTACC AccII 1398 CGCG CG^CG AcyI 20 GACGTC G[GA]^CG[CT]C Alw44I 1377 GTGCAC G^TGCAC AocI 200 CCTGAGG CC^T[ACGT]AGG ApaI 1266 GGGCCC GGGCC^C ApaLI 1377 GTGCAC G^TGCAC Asp718I 7 GGTACC G^GTACC AspI 1332 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 538 CCTCAGC CCTCAGC(-5/-2) BciVI 1354 GGATAC GTATCC(6/5) BglII 685 AGATCT A^GATCT BpmI 1420 CTCCAG CTGGAG(16/14) BsaHI 20 GACGTC G[GA]^CG[CT]C BsgI 449 GTGCAG GTGCAG(16/14) BsiEI 577 CGATCG CG[GA][CT]^CG BspHI 493 TCATGA T^CATGA BssSI 1235 CACGAG CACGAG(-5/-1) BstUI 1398 CGCG CG^CG BstXI 1041 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 685 AGATCT [GA]^GATC[CT] Bsu36I 200 CCTGAGG CC^T[ACGT]AGG Eco81I 200 CCTGAGG CC^T[ACGT]AGG EcoT22I 209 ATGCAT ATGCA^T FauI 832 GCGGG CCCGC(4/6) Hin1I 20 GACGTC G[GA]^CG[CT]C HindIII 653 AAGCTT A^AGCTT KpnI 7 GGTACC GGTAC^C MflI 685 AGATCT [GA]^GATC[CT] MseI 996 TTAA T^TAA MslI 641 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1398 CGCG CG^CG NaeI 955 GCCGGC GCC^GGC NdeI 1136 CATATG CA^TATG NgoMIV 955 GCCGGC G^CCGGC NsiI 209 ATGCAT ATGCA^T NspI 207 GCATGC [GA]CATG^[CT] PflFI 1332 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 618 AGGACCT [GA]G^G[AT]CC[CT] PshAI 275 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 577 CGATCG CGAT^CG RcaI 493 TCATGA T^CATGA SacI 1178 GAGCTC GAGCT^C SexAI 1161 ACCTGGT A^CC[AT]GGT SphI 207 GCATGC GCATG^C TaqI 364 TCGA T^CGA Tru9I 996 TTAA T^TAA Tsp509I 1394 AATT ^AATT Tth111I 1332 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 364 TCGA T^CGA XbaI 82 TCTAGA T^CTAGA XcmI 1119 CCACAGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 685 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AspHI 1178 GAGCTC G[AT]GC[AT]^C 1377 GTGCAC BfaI 83 CTAG C^TAG 740 CTAG BlpI 1105 GCTGAGC GC^T[ACGT]AGC 1307 GCTGAGC Bpu1102I 1105 GCTGAGC GC^T[ACGT]AGC 1307 GCTGAGC BsiHKAI 1178 GAGCTC G[AT]GC[AT]^C 1377 GTGCAC BsmBI 179 GAGACG CGTCTC(1/5) 721 GAGACG BsrFI 955 GCCGGC [GA]^CCGG[CT] 1230 ACCGGC CelII 1105 GCTGAGC GC^T[ACGT]AGC 1307 GCTGAGC Cfr10I 955 GCCGGC [GA]^CCGG[CT] 1230 ACCGGC EcoNI 189 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 790 CCTCCTACAGG EcoT14I 332 CCTTGG C^C[AT][AT]GG 1350 CCAAGG HaeII 374 GGCGCT [GA]GCGC^[CT] 1069 GGCGCT HgaI 181 GACGC GACGC(5/10) 500 GACGC HinfI 1325 GAATC G^A[ACGT]TC 1412 GAATC MaeI 83 CTAG C^TAG 740 CTAG MaeII 21 ACGT A^CGT 663 ACGT MspA1I 893 CAGCGG C[AC]G^C[GT]G 1025 CAGCGG SmlI 689 CTCAAG C^T[CT][GA]AG 1181 CTCAAG StuI 197 AGGCCT AGG^CCT 1188 AGGCCT StyI 332 CCTTGG C^C[AT][AT]GG 1350 CCAAGG TfiI 1325 GAATC G^A[AT]TC 1412 GAATC Tsp45I 273 GTGAC ^GT[GC]AC 1010 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI