##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AlwI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BcgI        BclI        
BfrI        BglI        BlnI        BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrDI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HincII      HindII      
HpaI        HphI        KasI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NarI        NcoI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuII       RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Van91I      XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII          20  GACGTC               GACGT^C
Acc65I          7  GGTACC               G^GTACC
AccII        1398  CGCG                 CG^CG
AcyI           20  GACGTC               G[GA]^CG[CT]C
Alw44I       1377  GTGCAC               G^TGCAC
AocI          200  CCTGAGG              CC^T[ACGT]AGG
ApaI         1266  GGGCCC               GGGCC^C
ApaLI        1377  GTGCAC               G^TGCAC
Asp718I         7  GGTACC               G^GTACC
AspI         1332  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI         538  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1354  GGATAC               GTATCC(6/5)
BglII         685  AGATCT               A^GATCT
BpmI         1420  CTCCAG               CTGGAG(16/14)
BsaHI          20  GACGTC               G[GA]^CG[CT]C
BsgI          449  GTGCAG               GTGCAG(16/14)
BsiEI         577  CGATCG               CG[GA][CT]^CG
BspHI         493  TCATGA               T^CATGA
BssSI        1235  CACGAG               CACGAG(-5/-1)
BstUI        1398  CGCG                 CG^CG
BstXI        1041  CCAGCTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         685  AGATCT               [GA]^GATC[CT]
Bsu36I        200  CCTGAGG              CC^T[ACGT]AGG
Eco81I        200  CCTGAGG              CC^T[ACGT]AGG
EcoT22I       209  ATGCAT               ATGCA^T
FauI          832  GCGGG                CCCGC(4/6)
Hin1I          20  GACGTC               G[GA]^CG[CT]C
HindIII       653  AAGCTT               A^AGCTT
KpnI            7  GGTACC               GGTAC^C
MflI          685  AGATCT               [GA]^GATC[CT]
MseI          996  TTAA                 T^TAA
MslI          641  CACATTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         1398  CGCG                 CG^CG
NaeI          955  GCCGGC               GCC^GGC
NdeI         1136  CATATG               CA^TATG
NgoMIV        955  GCCGGC               G^CCGGC
NsiI          209  ATGCAT               ATGCA^T
NspI          207  GCATGC               [GA]CATG^[CT]
PflFI        1332  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         618  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         275  GACACTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI          577  CGATCG               CGAT^CG
RcaI          493  TCATGA               T^CATGA
SacI         1178  GAGCTC               GAGCT^C
SexAI        1161  ACCTGGT              A^CC[AT]GGT
SphI          207  GCATGC               GCATG^C
TaqI          364  TCGA                 T^CGA
Tru9I         996  TTAA                 T^TAA
Tsp509I      1394  AATT                 ^AATT
Tth111I      1332  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       364  TCGA                 T^CGA
XbaI           82  TCTAGA               T^CTAGA
XcmI         1119  CCACAGAGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         685  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AspHI        1178  GAGCTC               G[AT]GC[AT]^C
             1377  GTGCAC              
BfaI           83  CTAG                 C^TAG
              740  CTAG                
BlpI         1105  GCTGAGC              GC^T[ACGT]AGC
             1307  GCTGAGC             
Bpu1102I     1105  GCTGAGC              GC^T[ACGT]AGC
             1307  GCTGAGC             
BsiHKAI      1178  GAGCTC               G[AT]GC[AT]^C
             1377  GTGCAC              
BsmBI         179  GAGACG               CGTCTC(1/5)
              721  GAGACG              
BsrFI         955  GCCGGC               [GA]^CCGG[CT]
             1230  ACCGGC              
CelII        1105  GCTGAGC              GC^T[ACGT]AGC
             1307  GCTGAGC             
Cfr10I        955  GCCGGC               [GA]^CCGG[CT]
             1230  ACCGGC              
EcoNI         189  CCTTCAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              790  CCTCCTACAGG         
EcoT14I       332  CCTTGG               C^C[AT][AT]GG
             1350  CCAAGG              
HaeII         374  GGCGCT               [GA]GCGC^[CT]
             1069  GGCGCT              
HgaI          181  GACGC                GACGC(5/10)
              500  GACGC               
HinfI        1325  GAATC                G^A[ACGT]TC
             1412  GAATC               
MaeI           83  CTAG                 C^TAG
              740  CTAG                
MaeII          21  ACGT                 A^CGT
              663  ACGT                
MspA1I        893  CAGCGG               C[AC]G^C[GT]G
             1025  CAGCGG              
SmlI          689  CTCAAG               C^T[CT][GA]AG
             1181  CTCAAG              
StuI          197  AGGCCT               AGG^CCT
             1188  AGGCCT              
StyI          332  CCTTGG               C^C[AT][AT]GG
             1350  CCAAGG              
TfiI         1325  GAATC                G^A[AT]TC
             1412  GAATC               
Tsp45I        273  GTGAC                ^GT[GC]AC
             1010  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TseI        
TspRI