##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
BspEI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tru9I Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 35 CGCG CG^CG
AcsI 116 GAATTT [GA]^AATT[CT]
AocI 229 CCTTAGG CC^T[ACGT]AGG
ApaI 322 GGGCCC GGGCC^C
ApoI 116 GAATTT [GA]^AATT[CT]
BsiEI 703 CGGCCG CG[GA][CT]^CG
Bsp1407I 712 TGTACA T^GTACA
BspDI 653 ATCGAT AT^CGAT
BspHI 595 TCATGA T^CATGA
BsrGI 712 TGTACA T^GTACA
BsrI 826 CCAGT ACTGG(1/-1)
BstUI 35 CGCG CG^CG
Bsu36I 229 CCTTAGG CC^T[ACGT]AGG
ClaI 653 ATCGAT AT^CGAT
DsaI 259 CCGTGG C^C[GA][CT]GG
EaeI 703 CGGCCG [CT]^GGCC[GA]
EagI 703 CGGCCG C^GGCCG
EarI 392 CTCTTC CTCTTC(1/4)
EclXI 703 CGGCCG C^GGCCG
Eco52I 703 CGGCCG C^GGCCG
Eco81I 229 CCTTAGG CC^T[ACGT]AGG
FauI 320 GCGGG CCCGC(4/6)
HgaI 462 GACGC GACGC(5/10)
HphI 971 GGTGA GGTGA(8/7)
Ksp632I 392 CTCTTC CTCTTC(1/4)
MvnI 35 CGCG CG^CG
NotI 702 GCGGCCGC GC^GGCCGC
PvuII 872 CAGCTG CAG^CTG
RcaI 595 TCATGA T^CATGA
SacI 543 GAGCTC GAGCT^C
SmaI 23 CCCGGG CCC^GGG
SspBI 712 TGTACA T^GTACA
TaqI 654 TCGA T^CGA
TfiI 30 GAATC G^A[AT]TC
TthHB8I 654 TCGA T^CGA
XbaI 587 TCTAGA T^CTAGA
XcmI 614 CCATGTGGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 23 CCCGGG C^CCGGG
XmaI 23 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 276 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
888 CAGTGTCTG
AspHI 543 GAGCTC G[AT]GC[AT]^C
1064 GTGCTC
AvaI 23 CCCGGG C^[CT]CG[GA]G
967 CTCGGG
BanII 322 GGGCCC G[GA]GC[CT]^C
543 GAGCTC
BbsI 1026 GAAGAC GAAGAC(2/6)
313 GTCTTC
BbvI 518 GCAGC GCAGC(8/12)
871 GCAGC
BfaI 216 CTAG C^TAG
588 CTAG
BpmI 354 CTCCAG CTGGAG(16/14)
540 CTGGAG
BpuAI 1026 GAAGAC GAAGAC(2/6)
313 GTCTTC
BsaI 820 GAGACC GGTCTC(1/5)
1164 GGTCTC
BsiHKAI 543 GAGCTC G[AT]GC[AT]^C
1064 GTGCTC
BsmFI 170 GGGAC GGGAC(10/14)
410 GGGAC
BsoBI 23 CCCGGG C^[CT]CG[GA]G
967 CTCGGG
BspMI 535 ACCTGC ACCTGC(4/8)
691 ACCTGC
BstXI 709 CCATGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1087 CCATCTTCATGG
HaeII 916 AGCGCC [GA]GCGC^[CT]
989 GGCGCT
MaeI 216 CTAG C^TAG
588 CTAG
MspA1I 318 CAGCGG C[AC]G^C[GT]G
872 CAGCTG
PflMI 83 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
275 CCAGGAGCTGG
PpuMI 211 AGGTCCT [GA]G^G[AT]CC[CT]
949 AGGACCC
SmlI 204 CTCAAG C^T[CT][GA]AG
1044 CTCAAG
TseI 518 GCAGC G^C[AT]GC
871 GCAGC
Tsp45I 831 GTGAC ^GT[GC]AC
1130 GTGAC
Van91I 83 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
275 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BcnI BglII BmyI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI Tsp509I TspRI XhoII