################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BglI BglII BlnI BpuAI BsaAI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII Bst1107I BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 643 GTAGAC GT^[AC][GT]AC AcyI 1262 GGCGCC G[GA]^CG[CT]C AflII 292 CTTAAG C^TTAAG AvaI 594 CTCGAG C^[CT]CG[GA]G BanI 1262 GGCGCC G^G[CT][GA]CC BbeI 1262 GGCGCC GGCGC^C BcgI 335 GCAGTACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 677 TGATCA T^GATCA BfrI 292 CTTAAG C^TTAAG BsaBI 1410 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1262 GGCGCC G[GA]^CG[CT]C BsaI 1109 GAGACC GGTCTC(1/5) BsoBI 594 CTCGAG C^[CT]CG[GA]G BspHI 1274 TCATGA T^CATGA BsrBI 1150 GAGCGG CCGCTC(-3/-3) BssSI 379 CACGAG CACGAG(-5/-1) BstAPI 378 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 299 TTCGAA TT^CGAA BstEII 1485 GGTGACC G^GT[ACGT]ACC BstPI 1485 GGTGACC G^GT[ACGT]ACC BstXI 1501 CCAGACACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 306 CCACGG C^C[GA][CT]GG EaeI 20 CGGCCA [CT]^GGCC[GA] EcoNI 807 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1485 GGTGACC G^GT[ACGT]ACC EcoT14I 1247 CCAAGG C^C[AT][AT]GG FbaI 677 TGATCA T^GATCA HgaI 797 GCGTC GACGC(5/10) Hin1I 1262 GGCGCC G[GA]^CG[CT]C KasI 1262 GGCGCC G^GCGCC MaeII 1128 ACGT A^CGT MamI 1410 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1262 GGCGCC GG^CGCC NspV 299 TTCGAA TT^CGAA PaeR7I 594 CTCGAG C^TCGAG PflMI 831 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1274 TCATGA T^CATGA SacI 96 GAGCTC GAGCT^C SexAI 1235 ACCTGGT A^CC[AT]GGT SfoI 1262 GGCGCC GGC^GCC SfuI 299 TTCGAA TT^CGAA SphI 924 GCATGC GCATG^C StyI 1247 CCAAGG C^C[AT][AT]GG Van91I 831 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 819 TCTAGA T^CTAGA XhoI 594 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 1394 AAATTT [GA]^AATT[CT] 1610 AAATTT AlwNI 277 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 832 CAGCTTCTG ApoI 1394 AAATTT [GA]^AATT[CT] 1610 AAATTT BlpI 996 GCTCAGC GC^T[ACGT]AGC 1341 GCTGAGC Bpu1102I 996 GCTCAGC GC^T[ACGT]AGC 1341 GCTGAGC BsmAI 479 GAGAC GTCTC(1/5) 1109 GAGAC BsrDI 491 CATTGC GCAATG(2/0) 1557 CATTGC CelII 996 GCTCAGC GC^T[ACGT]AGC 1341 GCTGAGC DpnII 678 GATC ^GATC 1140 GATC DraII 649 AGGTCCC [GA]G^G[ACGT]CC[CT] 1078 GGGACCC EcoO109I 649 AGGTCCC [GA]G^G[ACGT]CC[CT] 1078 GGGACCC HaeII 1202 GGCGCT [GA]GCGC^[CT] 1262 GGCGCC MboI 678 GATC ^GATC 1140 GATC NdeII 678 GATC ^GATC 1140 GATC PpuMI 649 AGGTCCC [GA]G^G[AT]CC[CT] 1078 GGGACCC PstI 385 CTGCAG CTGCA^G 621 CTGCAG PvuII 280 CAGCTG CAG^CTG 388 CAGCTG SapI 144 GAAGAGC GCTCTTC(1/4) 165 GAAGAGC Sau3AI 678 GATC ^GATC 1140 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV NspI PleI RsaI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I