##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BciVI BglI
BglII BlnI BpuAI BsaAI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrFI BsrGI BssHII Bst1107I
BstUI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FauI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XcmI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 643 GTAGAC GT^[AC][GT]AC
AcyI 1262 GGCGCC G[GA]^CG[CT]C
AflII 292 CTTAAG C^TTAAG
AvaI 594 CTCGAG C^[CT]CG[GA]G
BanI 1262 GGCGCC G^G[CT][GA]CC
BbeI 1262 GGCGCC GGCGC^C
BcgI 335 GCAGTACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 677 TGATCA T^GATCA
BfrI 292 CTTAAG C^TTAAG
BsaBI 1410 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1262 GGCGCC G[GA]^CG[CT]C
BsaI 1109 GAGACC GGTCTC(1/5)
BsoBI 594 CTCGAG C^[CT]CG[GA]G
BspHI 1274 TCATGA T^CATGA
BsrBI 1150 GAGCGG CCGCTC(-3/-3)
BssSI 379 CACGAG CACGAG(-5/-1)
BstAPI 378 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 299 TTCGAA TT^CGAA
BstEII 1485 GGTGACC G^GT[ACGT]ACC
BstPI 1485 GGTGACC G^GT[ACGT]ACC
BstXI 1501 CCAGACACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 306 CCACGG C^C[GA][CT]GG
EaeI 20 CGGCCA [CT]^GGCC[GA]
EcoNI 807 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1485 GGTGACC G^GT[ACGT]ACC
EcoT14I 1247 CCAAGG C^C[AT][AT]GG
FbaI 677 TGATCA T^GATCA
HgaI 797 GCGTC GACGC(5/10)
Hin1I 1262 GGCGCC G[GA]^CG[CT]C
KasI 1262 GGCGCC G^GCGCC
MaeII 1128 ACGT A^CGT
MamI 1410 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1262 GGCGCC GG^CGCC
NspV 299 TTCGAA TT^CGAA
PaeR7I 594 CTCGAG C^TCGAG
PflMI 831 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1274 TCATGA T^CATGA
SacI 96 GAGCTC GAGCT^C
SexAI 1235 ACCTGGT A^CC[AT]GGT
SfoI 1262 GGCGCC GGC^GCC
SfuI 299 TTCGAA TT^CGAA
SphI 924 GCATGC GCATG^C
StyI 1247 CCAAGG C^C[AT][AT]GG
Van91I 831 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 819 TCTAGA T^CTAGA
XhoI 594 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 1394 AAATTT [GA]^AATT[CT]
1610 AAATTT
AlwNI 277 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
832 CAGCTTCTG
ApoI 1394 AAATTT [GA]^AATT[CT]
1610 AAATTT
BlpI 996 GCTCAGC GC^T[ACGT]AGC
1341 GCTGAGC
Bpu1102I 996 GCTCAGC GC^T[ACGT]AGC
1341 GCTGAGC
BsmAI 479 GAGAC GTCTC(1/5)
1109 GAGAC
BsrDI 491 CATTGC GCAATG(2/0)
1557 CATTGC
CelII 996 GCTCAGC GC^T[ACGT]AGC
1341 GCTGAGC
DpnII 678 GATC ^GATC
1140 GATC
DraII 649 AGGTCCC [GA]G^G[ACGT]CC[CT]
1078 GGGACCC
EcoO109I 649 AGGTCCC [GA]G^G[ACGT]CC[CT]
1078 GGGACCC
HaeII 1202 GGCGCT [GA]GCGC^[CT]
1262 GGCGCC
MboI 678 GATC ^GATC
1140 GATC
NdeII 678 GATC ^GATC
1140 GATC
PpuMI 649 AGGTCCC [GA]G^G[AT]CC[CT]
1078 GGGACCC
PstI 385 CTGCAG CTGCA^G
621 CTGCAG
PvuII 280 CAGCTG CAG^CTG
388 CAGCTG
SapI 144 GAAGAGC GCTCTTC(1/4)
165 GAAGAGC
Sau3AI 678 GATC ^GATC
1140 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV NspI PleI RsaI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I