##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I Cfr10I
ClaI CpoI EagI Eam1105I EarI
EclXI Eco52I EcoNI EcoRI FbaI
FseI FspI HgaI HincII HindII
HpaI KpnI Ksp632I MfeI MflI
MluI MroI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I Tth111I XbaI XcmI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 642 GTCTAC GT^[AC][GT]AC
AocI 692 CCTCAGG CC^T[ACGT]AGG
Aor51HI 37 AGCGCT AGC^GCT
BalI 349 TGGCCA TGG^CCA
BcgI 986 CGAGGATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1052 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1052 GCTCAGC GC^T[ACGT]AGC
BsaBI 198 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 132 CTGCAC GTGCAG(16/14)
BsmAI 234 GAGAC GTCTC(1/5)
BsmBI 234 GAGACG CGTCTC(1/5)
BsmFI 868 GGGAC GGGAC(10/14)
BspMI 527 GCAGGT ACCTGC(4/8)
BstEII 389 GGTCACC G^GT[ACGT]ACC
BstPI 389 GGTCACC G^GT[ACGT]ACC
Bsu36I 692 CCTCAGG CC^T[ACGT]AGG
CelII 1052 GCTCAGC GC^T[ACGT]AGC
DraI 1286 TTTAAA TTT^AAA
DraIII 914 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 377 GACAGAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 37 AGCGCT AGC^GCT
Eco81I 692 CCTCAGG CC^T[ACGT]AGG
EcoO65I 389 GGTCACC G^GT[ACGT]ACC
EcoRV 594 GATATC GAT^ATC
EcoT14I 997 CCAAGG C^C[AT][AT]GG
EcoT22I 636 ATGCAT ATGCA^T
HindIII 1273 AAGCTT A^AGCTT
KspI 1115 CCGCGG CCGC^GG
MaeII 1162 ACGT A^CGT
MaeIII 390 GTCAC ^GT[ACGT]AC
MamI 198 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 349 TGGCCA TGG^CCA
MscI 349 TGGCCA TGG^CCA
MseI 1287 TTAA T^TAA
NsiI 636 ATGCAT ATGCA^T
PvuII 1137 CAGCTG CAG^CTG
SacII 1115 CCGCGG CCGC^GG
SfcI 844 CTGTAG C^T[GA][CT]AG
SfiI 308 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 316 AGGCCT AGG^CCT
StyI 997 CCAAGG C^C[AT][AT]GG
TfiI 849 GAATC G^A[AT]TC
Tru9I 1287 TTAA T^TAA
Tsp45I 390 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 11 CGCG CG^CG
1116 CGCG
AfaI 509 GTAC GT^AC
1011 GTAC
AspHI 1050 GAGCTC G[AT]GC[AT]^C
1074 GAGCTC
AvaI 358 CCCGGG C^[CT]CG[GA]G
720 CCCGGG
BanII 1050 GAGCTC G[GA]GC[CT]^C
1074 GAGCTC
BbsI 1095 GAAGAC GAAGAC(2/6)
570 GTCTTC
BfaI 300 CTAG C^TAG
615 CTAG
BglI 309 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
834 GCCACTGGGGC
BpuAI 1095 GAAGAC GAAGAC(2/6)
570 GTCTTC
BsiHKAI 1050 GAGCTC G[AT]GC[AT]^C
1074 GAGCTC
BsoBI 358 CCCGGG C^[CT]CG[GA]G
720 CCCGGG
BsrBI 84 GAGCGG CCGCTC(-3/-3)
935 GAGCGG
BsrDI 829 GCAATG GCAATG(2/0)
955 GCAATG
BstUI 11 CGCG CG^CG
1116 CGCG
DpnII 215 GATC ^GATC
1269 GATC
DsaI 872 CCGTGG C^C[GA][CT]GG
1115 CCGCGG
MaeI 300 CTAG C^TAG
615 CTAG
MboI 215 GATC ^GATC
1269 GATC
MvnI 11 CGCG CG^CG
1116 CGCG
NdeII 215 GATC ^GATC
1269 GATC
NspI 634 ACATGC [GA]CATG^[CT]
638 GCATGT
PleI 455 GACTC GAGTC(4/5)
1194 GACTC
RsaI 509 GTAC GT^AC
1011 GTAC
SacI 1050 GAGCTC GAGCT^C
1074 GAGCTC
Sau3AI 215 GATC ^GATC
1269 GATC
SmaI 358 CCCGGG CCC^GGG
720 CCCGGG
XmaCI 358 CCCGGG C^CCGGG
720 CCCGGG
XmaI 358 CCCGGG C^CCGGG
720 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcyI AluI AvaII BanI
BbeI BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MboII MnlI MspA1I
MspI MvaI MwoI NarI NciI
NlaIII NlaIV PflMI PpuMI Sau96I
ScrFI SfaNI SfoI TaqI TseI
TspRI TthHB8I Van91I