##################################
# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbsI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT14I EcoT22I FauI FokI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI HphI KasI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PleI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 289 GGTACC G^GTACC
AccI 508 GTCTAC GT^[AC][GT]AC
AciI 81 CCGC CCGC(-3/-1)
Asp718I 289 GGTACC G^GTACC
AspI 248 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 289 GGTACC G^G[CT][GA]CC
BbrPI 122 CACGTG CAC^GTG
BbvI 43 GCTGC GCAGC(8/12)
BclI 357 TGATCA T^GATCA
BcnI 221 CCGGG CC^[GC]GG
BfaI 462 CTAG C^TAG
BsaAI 122 CACGTG [CT]AC^GT[GA]
BseRI 2 GAGGAG GAGGAG(10/8)
BsmFI 263 GTCCC GGGAC(10/14)
BsmI 310 GCATTC GAATGC(1/-1)
BsrDI 98 GCAATG GCAATG(2/0)
BssSI 179 CACGAG CACGAG(-5/-1)
BstNI 112 CCTGG CC^[AT]GG
CfoI 96 GCGC GCG^C
DdeI 483 CTGAG C^T[ACGT]AG
DraI 15 TTTAAA TTT^AAA
DraII 108 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 179 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 175 CCTCCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 108 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRII 112 CCTGG ^CC[AT]GG
EcoRV 88 GATATC GAT^ATC
FbaI 357 TGATCA T^GATCA
Fnu4HI 43 GCTGC GC^[ACGT]GC
HaeIII 170 GGCC GG^CC
HapII 221 CCGG C^CGG
HgaI 252 GCGTC GACGC(5/10)
HhaI 96 GCGC GCG^C
HinP1I 96 GCGC G^CGC
HinfI 438 GATTC G^A[ACGT]TC
HpaII 221 CCGG C^CGG
ItaI 43 GCTGC GC^[ACGT]GC
KpnI 289 GGTACC GGTAC^C
MaeI 462 CTAG C^TAG
MspI 221 CCGG C^CGG
MvaI 112 CCTGG CC^[AT]GG
NciI 221 CCGGG CC^[GC]GG
NlaIII 189 CATG CATG^
PflFI 248 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 178 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 122 CACGTG CAC^GTG
PmlI 122 CACGTG CAC^GTG
PpuMI 108 GGGTCCT [GA]G^G[AT]CC[CT]
TfiI 438 GATTC G^A[AT]TC
TseI 43 GCTGC G^C[AT]GC
Tth111I 248 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 178 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 10 GTAC GT^AC
290 GTAC
AluI 42 AGCT AG^CT
460 AGCT
AvaII 109 GGTCC G^G[AT]CC
118 GGTCC
BssKI 112 CCTGG ^CC[ACGT]GG
221 CCGGG
MaeII 12 ACGT A^CGT
123 ACGT
MaeIII 158 GTGAC ^GT[ACGT]AC
342 GTGAC
MwoI 74 GCGATGACCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
275 GCAGTTTTGGC
RsaI 10 GTAC GT^AC
290 GTAC
ScrFI 112 CCTGG CC^[ACGT]GG
221 CCGGG
SfaNI 147 GATGC GCATC(5/9)
380 GCATC
Tsp45I 158 GTGAC ^GT[GC]AC
342 GTGAC
TspRI 267 CACTG CA[GC]TG[ACGT][ACGT]^
285 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
BsiYI BslI Cfr13I DpnII EarI
Ksp632I MboI MboII MnlI MseI
NdeII NlaIV Sau3AI Sau96I Tru9I
Tsp509I