##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaII AviII BalI
BamHI BanII BbrPI BbvCI BcgI
BciVI BglI BlpI Bpu1102I BsaBI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BspDI BspEI BspHI BspLU11I
BspMI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRV
FauI FseI FspI HgaI HindIII
HpaI KpnI Ksp632I KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1526 GACGTC GACGT^C
AflII 1020 CTTAAG C^TTAAG
AflIII 13 ACACGT A^C[GA][CT]GT
AlwNI 831 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1571 CCTTAGG CC^T[ACGT]AGG
AvaI 356 CTCGGG C^[CT]CG[GA]G
AvrII 173 CCTAGG C^CTAGG
BanI 914 GGCGCC G^G[CT][GA]CC
BbeI 914 GGCGCC GGCGC^C
BclI 8 TGATCA T^GATCA
BcnI 408 CCCGG CC^[GC]GG
BfrI 1020 CTTAAG C^TTAAG
BlnI 173 CCTAGG C^CTAGG
BpmI 25 CTGGAG CTGGAG(16/14)
BsaAI 1427 TACGTG [CT]AC^GT[GA]
BsaWI 447 TCCGGT [AT]^CCGG[AT]
BsmAI 499 GAGAC GTCTC(1/5)
BsmBI 499 GAGACG CGTCTC(1/5)
BsoBI 356 CTCGGG C^[CT]CG[GA]G
Bsp1407I 838 TGTACA T^GTACA
BsrBI 1121 CCGCTC CCGCTC(-3/-3)
BsrGI 838 TGTACA T^GTACA
BssSI 271 CTCGTG CACGAG(-5/-1)
Bsu36I 1571 CCTTAGG CC^T[ACGT]AGG
DraI 1187 TTTAAA TTT^AAA
DsaI 633 CCGTGG C^C[GA][CT]GG
Eco81I 1571 CCTTAGG CC^T[ACGT]AGG
EcoRI 1037 GAATTC G^AATTC
FbaI 8 TGATCA T^GATCA
HaeII 914 GGCGCC [GA]GCGC^[CT]
HincII 1354 GTTGAC GT[CT]^[GA]AC
HindII 1354 GTTGAC GT[CT]^[GA]AC
KasI 914 GGCGCC G^GCGCC
MaeIII 660 GTTAC ^GT[ACGT]AC
MfeI 731 CAATTG C^AATTG
MunI 731 CAATTG C^AATTG
NarI 914 GGCGCC GG^CGCC
NciI 408 CCCGG CC^[GC]GG
PleI 1641 GAGTC GAGTC(4/5)
SfoI 914 GGCGCC GGC^GCC
SspBI 838 TGTACA T^GTACA
StuI 852 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 1121 CCGC CCGC(-3/-1)
458 GCGG
AcyI 914 GGCGCC G[GA]^CG[CT]C
1526 GACGTC
AfaI 511 GTAC GT^AC
839 GTAC
Asp700I 241 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1323 GAAGAAATTC
BbsI 294 GAAGAC GAAGAC(2/6)
461 GAAGAC
BpuAI 294 GAAGAC GAAGAC(2/6)
461 GAAGAC
BsaHI 914 GGCGCC G[GA]^CG[CT]C
1526 GACGTC
BsmFI 652 GGGAC GGGAC(10/14)
522 GTCCC
Cac8I 257 GCAGGC GC[ACGT]^[ACGT]GC
875 GCTGGC
CfoI 891 GCGC GCG^C
915 GCGC
EcoT14I 173 CCTAGG C^C[AT][AT]GG
192 CCTTGG
EcoT22I 1492 ATGCAT ATGCA^T
1674 ATGCAT
HaeIII 853 GGCC GG^CC
959 GGCC
HapII 409 CCGG C^CGG
448 CCGG
HhaI 891 GCGC GCG^C
915 GCGC
Hin1I 914 GGCGCC G[GA]^CG[CT]C
1526 GACGTC
HinP1I 891 GCGC G^CGC
915 GCGC
HpaII 409 CCGG C^CGG
448 CCGG
MslI 882 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1677 CATTATTGTG
MspA1I 370 CAGCTG C[AC]G^C[GT]G
831 CAGCTG
MspI 409 CCGG C^CGG
448 CCGG
NsiI 1492 ATGCAT ATGCA^T
1674 ATGCAT
PvuII 370 CAGCTG CAG^CTG
831 CAGCTG
RsaI 511 GTAC GT^AC
839 GTAC
SmlI 717 CTTGAG C^T[CT][GA]AG
1020 CTTAAG
StyI 173 CCTAGG C^C[AT][AT]GG
192 CCTTGG
XmnI 241 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1323 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccI AcsI AluI AlwI ApoI
BbvI BfaI BglII BmyI BsaJI
BseRI BsiYI BslI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI DdeI DpnII EcoRII Fnu4HI
FokI HinfI HphI ItaI MaeI
MaeII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII