################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbrPI BbsI BcgI BcnI BfrI BglI BlnI BpuAI BsaAI BsiEI BsiWI BsmBI BsmI BsoBI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HhaI HinP1I HpaI KasI KpnI KspI MluI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 1614 GTCTAC GT^[AC][GT]AC AciI 629 GCGG CCGC(-3/-1) AcyI 1933 GACGCC G[GA]^CG[CT]C ApaI 1578 GGGCCC GGGCC^C AseI 2132 ATTAAT AT^TAAT AsnI 2132 ATTAAT AT^TAAT BalI 841 TGGCCA TGG^CCA BamHI 806 GGATCC G^GATCC BbvCI 1937 CCTCAGC CCTCAGC(-5/-2) BciVI 1821 GTATCC GTATCC(6/5) BclI 1492 TGATCA T^GATCA BlpI 1320 GCTTAGC GC^T[ACGT]AGC BpmI 2175 CTCCAG CTGGAG(16/14) Bpu1102I 1320 GCTTAGC GC^T[ACGT]AGC BsaBI 2114 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1933 GACGCC G[GA]^CG[CT]C BseRI 1131 CTCCTC GAGGAG(10/8) BsmFI 1642 GGGAC GGGAC(10/14) Bsp1407I 2167 TGTACA T^GTACA BspHI 348 TCATGA T^CATGA BsrDI 1844 GCAATG GCAATG(2/0) BsrGI 2167 TGTACA T^GTACA CelII 1320 GCTTAGC GC^T[ACGT]AGC DraI 1228 TTTAAA TTT^AAA DraII 1577 AGGGCCC [GA]G^G[ACGT]CC[CT] DsaI 1667 CCATGG C^C[GA][CT]GG EcoO109I 1577 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRV 2126 GATATC GAT^ATC FbaI 1492 TGATCA T^GATCA HgaI 1933 GACGC GACGC(5/10) Hin1I 1933 GACGCC G[GA]^CG[CT]C MaeII 107 ACGT A^CGT MamI 2114 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1011 CAATTG C^AATTG MluNI 841 TGGCCA TGG^CCA MscI 841 TGGCCA TGG^CCA MspA1I 417 CAGCTG C[AC]G^C[GT]G MunI 1011 CAATTG C^AATTG NcoI 1667 CCATGG C^CATGG NdeI 1041 CATATG CA^TATG PflMI 773 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 855 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2132 ATTAAT AT^TAAT PvuII 417 CAGCTG CAG^CTG RcaI 348 TCATGA T^CATGA SacI 1657 GAGCTC GAGCT^C SpeI 794 ACTAGT A^CTAGT SspBI 2167 TGTACA T^GTACA Van91I 773 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccIII 25 TCCGGA T^CCGGA 85 TCCGGA AcsI 535 AAATTT [GA]^AATT[CT] 2035 AAATTT AflIII 1547 ACATGT A^C[GA][CT]GT 1779 ACATGT ApoI 535 AAATTT [GA]^AATT[CT] 2035 AAATTT BsaI 1267 GAGACC GGTCTC(1/5) 1430 GAGACC BseAI 25 TCCGGA T^CCGGA 85 TCCGGA BsmAI 1267 GAGAC GTCTC(1/5) 1430 GAGAC BspDI 1028 ATCGAT AT^CGAT 1562 ATCGAT BspEI 25 TCCGGA T^CCGGA 85 TCCGGA BspLU11I 1547 ACATGT A^CATGT 1779 ACATGT ClaI 1028 ATCGAT AT^CGAT 1562 ATCGAT EaeI 55 CGGCCA [CT]^GGCC[GA] 841 TGGCCA EcoT14I 1667 CCATGG C^C[AT][AT]GG 1855 CCAAGG HincII 334 GTTGAC GT[CT]^[GA]AC 1888 GTCAAC HindII 334 GTTGAC GT[CT]^[GA]AC 1888 GTCAAC HindIII 240 AAGCTT A^AGCTT 262 AAGCTT HphI 980 GGTGA GGTGA(8/7) 1402 GGTGA MroI 25 TCCGGA T^CCGGA 85 TCCGGA MslI 349 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 923 CATCCTGATG StyI 1667 CCATGG C^C[AT][AT]GG 1855 CCAAGG XcmI 105 CCACGTTTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2028 CCACTGAAAATTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AfaI AluI AlwI AlwNI AspHI AvaII BanII BbvI BfaI BglII BmyI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII