##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BanI        
BbeI        BbrPI       BbsI        BcgI        BcnI        
BfrI        BglI        BlnI        BpuAI       BsaAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CfoI        
Cfr10I      CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT22I     FauI        
FseI        FspI        HaeII       HhaI        HinP1I      
HpaI        KasI        KpnI        KspI        MluI        
MvnI        NaeI        NarI        NciI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SphI        
Sse8387I    SspI        StuI        SwaI        Tsp45I      
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         1614  GTCTAC               GT^[AC][GT]AC
AciI          629  GCGG                 CCGC(-3/-1)
AcyI         1933  GACGCC               G[GA]^CG[CT]C
ApaI         1578  GGGCCC               GGGCC^C
AseI         2132  ATTAAT               AT^TAAT
AsnI         2132  ATTAAT               AT^TAAT
BalI          841  TGGCCA               TGG^CCA
BamHI         806  GGATCC               G^GATCC
BbvCI        1937  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1821  GTATCC               GTATCC(6/5)
BclI         1492  TGATCA               T^GATCA
BlpI         1320  GCTTAGC              GC^T[ACGT]AGC
BpmI         2175  CTCCAG               CTGGAG(16/14)
Bpu1102I     1320  GCTTAGC              GC^T[ACGT]AGC
BsaBI        2114  GATCTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1933  GACGCC               G[GA]^CG[CT]C
BseRI        1131  CTCCTC               GAGGAG(10/8)
BsmFI        1642  GGGAC                GGGAC(10/14)
Bsp1407I     2167  TGTACA               T^GTACA
BspHI         348  TCATGA               T^CATGA
BsrDI        1844  GCAATG               GCAATG(2/0)
BsrGI        2167  TGTACA               T^GTACA
CelII        1320  GCTTAGC              GC^T[ACGT]AGC
DraI         1228  TTTAAA               TTT^AAA
DraII        1577  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DsaI         1667  CCATGG               C^C[GA][CT]GG
EcoO109I     1577  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRV        2126  GATATC               GAT^ATC
FbaI         1492  TGATCA               T^GATCA
HgaI         1933  GACGC                GACGC(5/10)
Hin1I        1933  GACGCC               G[GA]^CG[CT]C
MaeII         107  ACGT                 A^CGT
MamI         2114  GATCTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1011  CAATTG               C^AATTG
MluNI         841  TGGCCA               TGG^CCA
MscI          841  TGGCCA               TGG^CCA
MspA1I        417  CAGCTG               C[AC]G^C[GT]G
MunI         1011  CAATTG               C^AATTG
NcoI         1667  CCATGG               C^CATGG
NdeI         1041  CATATG               CA^TATG
PflMI         773  CCACCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         855  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2132  ATTAAT               AT^TAAT
PvuII         417  CAGCTG               CAG^CTG
RcaI          348  TCATGA               T^CATGA
SacI         1657  GAGCTC               GAGCT^C
SpeI          794  ACTAGT               A^CTAGT
SspBI        2167  TGTACA               T^GTACA
Van91I        773  CCACCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccIII         25  TCCGGA               T^CCGGA
               85  TCCGGA              
AcsI          535  AAATTT               [GA]^AATT[CT]
             2035  AAATTT              
AflIII       1547  ACATGT               A^C[GA][CT]GT
             1779  ACATGT              
ApoI          535  AAATTT               [GA]^AATT[CT]
             2035  AAATTT              
BsaI         1267  GAGACC               GGTCTC(1/5)
             1430  GAGACC              
BseAI          25  TCCGGA               T^CCGGA
               85  TCCGGA              
BsmAI        1267  GAGAC                GTCTC(1/5)
             1430  GAGAC               
BspDI        1028  ATCGAT               AT^CGAT
             1562  ATCGAT              
BspEI          25  TCCGGA               T^CCGGA
               85  TCCGGA              
BspLU11I     1547  ACATGT               A^CATGT
             1779  ACATGT              
ClaI         1028  ATCGAT               AT^CGAT
             1562  ATCGAT              
EaeI           55  CGGCCA               [CT]^GGCC[GA]
              841  TGGCCA              
EcoT14I      1667  CCATGG               C^C[AT][AT]GG
             1855  CCAAGG              
HincII        334  GTTGAC               GT[CT]^[GA]AC
             1888  GTCAAC              
HindII        334  GTTGAC               GT[CT]^[GA]AC
             1888  GTCAAC              
HindIII       240  AAGCTT               A^AGCTT
              262  AAGCTT              
HphI          980  GGTGA                GGTGA(8/7)
             1402  GGTGA               
MroI           25  TCCGGA               T^CCGGA
               85  TCCGGA              
MslI          349  CATGAAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              923  CATCCTGATG          
StyI         1667  CCATGG               C^C[AT][AT]GG
             1855  CCAAGG              
XcmI          105  CCACGTTTTTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2028  CCACTGAAAATTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AfaI        AluI        AlwI        AlwNI       AspHI       
AvaII       BanII       BbvI        BfaI        BglII       
BmyI        BsaJI       BsaWI       BsgI        BsiHKAI     
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII