##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbsI BcgI BcnI
BfrI BglI BlnI BpuAI BsaAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr10I CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FseI FspI HaeII HhaI HinP1I
HpaI KasI KpnI KspI MluI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspI StuI SwaI Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1614 GTCTAC GT^[AC][GT]AC
AciI 629 GCGG CCGC(-3/-1)
AcyI 1933 GACGCC G[GA]^CG[CT]C
ApaI 1578 GGGCCC GGGCC^C
AseI 2132 ATTAAT AT^TAAT
AsnI 2132 ATTAAT AT^TAAT
BalI 841 TGGCCA TGG^CCA
BamHI 806 GGATCC G^GATCC
BbvCI 1937 CCTCAGC CCTCAGC(-5/-2)
BciVI 1821 GTATCC GTATCC(6/5)
BclI 1492 TGATCA T^GATCA
BlpI 1320 GCTTAGC GC^T[ACGT]AGC
BpmI 2175 CTCCAG CTGGAG(16/14)
Bpu1102I 1320 GCTTAGC GC^T[ACGT]AGC
BsaBI 2114 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1933 GACGCC G[GA]^CG[CT]C
BseRI 1131 CTCCTC GAGGAG(10/8)
BsmFI 1642 GGGAC GGGAC(10/14)
Bsp1407I 2167 TGTACA T^GTACA
BspHI 348 TCATGA T^CATGA
BsrDI 1844 GCAATG GCAATG(2/0)
BsrGI 2167 TGTACA T^GTACA
CelII 1320 GCTTAGC GC^T[ACGT]AGC
DraI 1228 TTTAAA TTT^AAA
DraII 1577 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 1667 CCATGG C^C[GA][CT]GG
EcoO109I 1577 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 2126 GATATC GAT^ATC
FbaI 1492 TGATCA T^GATCA
HgaI 1933 GACGC GACGC(5/10)
Hin1I 1933 GACGCC G[GA]^CG[CT]C
MaeII 107 ACGT A^CGT
MamI 2114 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1011 CAATTG C^AATTG
MluNI 841 TGGCCA TGG^CCA
MscI 841 TGGCCA TGG^CCA
MspA1I 417 CAGCTG C[AC]G^C[GT]G
MunI 1011 CAATTG C^AATTG
NcoI 1667 CCATGG C^CATGG
NdeI 1041 CATATG CA^TATG
PflMI 773 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 855 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2132 ATTAAT AT^TAAT
PvuII 417 CAGCTG CAG^CTG
RcaI 348 TCATGA T^CATGA
SacI 1657 GAGCTC GAGCT^C
SpeI 794 ACTAGT A^CTAGT
SspBI 2167 TGTACA T^GTACA
Van91I 773 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 25 TCCGGA T^CCGGA
85 TCCGGA
AcsI 535 AAATTT [GA]^AATT[CT]
2035 AAATTT
AflIII 1547 ACATGT A^C[GA][CT]GT
1779 ACATGT
ApoI 535 AAATTT [GA]^AATT[CT]
2035 AAATTT
BsaI 1267 GAGACC GGTCTC(1/5)
1430 GAGACC
BseAI 25 TCCGGA T^CCGGA
85 TCCGGA
BsmAI 1267 GAGAC GTCTC(1/5)
1430 GAGAC
BspDI 1028 ATCGAT AT^CGAT
1562 ATCGAT
BspEI 25 TCCGGA T^CCGGA
85 TCCGGA
BspLU11I 1547 ACATGT A^CATGT
1779 ACATGT
ClaI 1028 ATCGAT AT^CGAT
1562 ATCGAT
EaeI 55 CGGCCA [CT]^GGCC[GA]
841 TGGCCA
EcoT14I 1667 CCATGG C^C[AT][AT]GG
1855 CCAAGG
HincII 334 GTTGAC GT[CT]^[GA]AC
1888 GTCAAC
HindII 334 GTTGAC GT[CT]^[GA]AC
1888 GTCAAC
HindIII 240 AAGCTT A^AGCTT
262 AAGCTT
HphI 980 GGTGA GGTGA(8/7)
1402 GGTGA
MroI 25 TCCGGA T^CCGGA
85 TCCGGA
MslI 349 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
923 CATCCTGATG
StyI 1667 CCATGG C^C[AT][AT]GG
1855 CCAAGG
XcmI 105 CCACGTTTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2028 CCACTGAAAATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AfaI AluI AlwI AlwNI AspHI
AvaII BanII BbvI BfaI BglII
BmyI BsaJI BsaWI BsgI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII