##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
ApaI AscI Asp700I AspEI AspI
AviII AvrII BbrPI BbvCI BcgI
BciVI BclI BglII BlnI BlpI
Bpu1102I BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BsrBI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FbaI
FseI FspI HindIII HpaI KspI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 536 GGTACC G^GTACC
AccII 1723 CGCG CG^CG
AccIII 1036 TCCGGA T^CCGGA
AcyI 1248 GGCGCC G[GA]^CG[CT]C
AflII 657 CTTAAG C^TTAAG
AocI 554 CCTGAGG CC^T[ACGT]AGG
Aor51HI 43 AGCGCT AGC^GCT
AseI 2328 ATTAAT AT^TAAT
AsnI 2328 ATTAAT AT^TAAT
Asp718I 536 GGTACC G^GTACC
BamHI 1527 GGATCC G^GATCC
BbeI 1248 GGCGCC GGCGC^C
BfrI 657 CTTAAG C^TTAAG
BglI 1210 GCCCCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 276 TACGTG [CT]AC^GT[GA]
BsaHI 1248 GGCGCC G[GA]^CG[CT]C
BsaI 1025 GGTCTC GGTCTC(1/5)
BsaWI 1036 TCCGGA [AT]^CCGG[AT]
BseAI 1036 TCCGGA T^CCGGA
BspEI 1036 TCCGGA T^CCGGA
BsrFI 262 ACCGGC [GA]^CCGG[CT]
BstUI 1723 CGCG CG^CG
BstXI 289 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 554 CCTGAGG CC^T[ACGT]AGG
Cfr10I 262 ACCGGC [GA]^CCGG[CT]
Eco47III 43 AGCGCT AGC^GCT
Eco81I 554 CCTGAGG CC^T[ACGT]AGG
EcoNI 1886 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2279 ATGCAT ATGCA^T
Hin1I 1248 GGCGCC G[GA]^CG[CT]C
HincII 49 GTCAAC GT[CT]^[GA]AC
HindII 49 GTCAAC GT[CT]^[GA]AC
KasI 1248 GGCGCC G^GCGCC
KpnI 536 GGTACC GGTAC^C
MaeII 277 ACGT A^CGT
MroI 1036 TCCGGA T^CCGGA
MvnI 1723 CGCG CG^CG
NarI 1248 GGCGCC GG^CGCC
NsiI 2279 ATGCAT ATGCA^T
PshBI 2328 ATTAAT AT^TAAT
SapI 671 GAAGAGC GCTCTTC(1/4)
SfoI 1248 GGCGCC GGC^GCC
SmaI 1676 CCCGGG CCC^GGG
SphI 2144 GCATGC GCATG^C
XbaI 1310 TCTAGA T^CTAGA
XcmI 380 CCAAATCTTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1676 CCCGGG C^CCGGG
XmaI 1676 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 708 GAATTC [GA]^AATT[CT]
776 GAATTC
AflIII 132 ACATGT A^C[GA][CT]GT
1550 ACATGT
Alw44I 233 GTGCAC G^TGCAC
1735 GTGCAC
ApaLI 233 GTGCAC G^TGCAC
1735 GTGCAC
ApoI 708 GAATTC [GA]^AATT[CT]
776 GAATTC
AvaI 1272 CCCGAG C^[CT]CG[GA]G
1676 CCCGGG
BbsI 432 GAAGAC GAAGAC(2/6)
1509 GTCTTC
BcnI 1676 CCCGG CC^[GC]GG
1677 CCGGG
BpuAI 432 GAAGAC GAAGAC(2/6)
1509 GTCTTC
BsmAI 1026 GTCTC GTCTC(1/5)
2025 GTCTC
BsoBI 1272 CCCGAG C^[CT]CG[GA]G
1676 CCCGGG
BspHI 475 TCATGA T^CATGA
1387 TCATGA
BspLU11I 132 ACATGT A^CATGT
1550 ACATGT
BspMI 872 GCAGGT ACCTGC(4/8)
2065 GCAGGT
BssSI 879 CACGAG CACGAG(-5/-1)
1189 CTCGTG
EcoRI 708 GAATTC G^AATTC
776 GAATTC
FauI 415 CCCGC CCCGC(4/6)
1945 GCGGG
HgaI 208 GCGTC GACGC(5/10)
698 GCGTC
NciI 1676 CCCGG CC^[GC]GG
1677 CCGGG
PvuII 17 CAGCTG CAG^CTG
693 CAGCTG
RcaI 475 TCATGA T^CATGA
1387 TCATGA
TfiI 1301 GAATC G^A[AT]TC
1556 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII