################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII AccI AclI AgeI AhdI ApaI AscI Asp700I AspEI AspI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BglII BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 536 GGTACC G^GTACC AccII 1723 CGCG CG^CG AccIII 1036 TCCGGA T^CCGGA AcyI 1248 GGCGCC G[GA]^CG[CT]C AflII 657 CTTAAG C^TTAAG AocI 554 CCTGAGG CC^T[ACGT]AGG Aor51HI 43 AGCGCT AGC^GCT AseI 2328 ATTAAT AT^TAAT AsnI 2328 ATTAAT AT^TAAT Asp718I 536 GGTACC G^GTACC BamHI 1527 GGATCC G^GATCC BbeI 1248 GGCGCC GGCGC^C BfrI 657 CTTAAG C^TTAAG BglI 1210 GCCCCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 276 TACGTG [CT]AC^GT[GA] BsaHI 1248 GGCGCC G[GA]^CG[CT]C BsaI 1025 GGTCTC GGTCTC(1/5) BsaWI 1036 TCCGGA [AT]^CCGG[AT] BseAI 1036 TCCGGA T^CCGGA BspEI 1036 TCCGGA T^CCGGA BsrFI 262 ACCGGC [GA]^CCGG[CT] BstUI 1723 CGCG CG^CG BstXI 289 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 554 CCTGAGG CC^T[ACGT]AGG Cfr10I 262 ACCGGC [GA]^CCGG[CT] Eco47III 43 AGCGCT AGC^GCT Eco81I 554 CCTGAGG CC^T[ACGT]AGG EcoNI 1886 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2279 ATGCAT ATGCA^T Hin1I 1248 GGCGCC G[GA]^CG[CT]C HincII 49 GTCAAC GT[CT]^[GA]AC HindII 49 GTCAAC GT[CT]^[GA]AC KasI 1248 GGCGCC G^GCGCC KpnI 536 GGTACC GGTAC^C MaeII 277 ACGT A^CGT MroI 1036 TCCGGA T^CCGGA MvnI 1723 CGCG CG^CG NarI 1248 GGCGCC GG^CGCC NsiI 2279 ATGCAT ATGCA^T PshBI 2328 ATTAAT AT^TAAT SapI 671 GAAGAGC GCTCTTC(1/4) SfoI 1248 GGCGCC GGC^GCC SmaI 1676 CCCGGG CCC^GGG SphI 2144 GCATGC GCATG^C XbaI 1310 TCTAGA T^CTAGA XcmI 380 CCAAATCTTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1676 CCCGGG C^CCGGG XmaI 1676 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 708 GAATTC [GA]^AATT[CT] 776 GAATTC AflIII 132 ACATGT A^C[GA][CT]GT 1550 ACATGT Alw44I 233 GTGCAC G^TGCAC 1735 GTGCAC ApaLI 233 GTGCAC G^TGCAC 1735 GTGCAC ApoI 708 GAATTC [GA]^AATT[CT] 776 GAATTC AvaI 1272 CCCGAG C^[CT]CG[GA]G 1676 CCCGGG BbsI 432 GAAGAC GAAGAC(2/6) 1509 GTCTTC BcnI 1676 CCCGG CC^[GC]GG 1677 CCGGG BpuAI 432 GAAGAC GAAGAC(2/6) 1509 GTCTTC BsmAI 1026 GTCTC GTCTC(1/5) 2025 GTCTC BsoBI 1272 CCCGAG C^[CT]CG[GA]G 1676 CCCGGG BspHI 475 TCATGA T^CATGA 1387 TCATGA BspLU11I 132 ACATGT A^CATGT 1550 ACATGT BspMI 872 GCAGGT ACCTGC(4/8) 2065 GCAGGT BssSI 879 CACGAG CACGAG(-5/-1) 1189 CTCGTG EcoRI 708 GAATTC G^AATTC 776 GAATTC FauI 415 CCCGC CCCGC(4/6) 1945 GCGGG HgaI 208 GCGTC GACGC(5/10) 698 GCGTC NciI 1676 CCCGG CC^[GC]GG 1677 CCGGG PvuII 17 CAGCTG CAG^CTG 693 CAGCTG RcaI 475 TCATGA T^CATGA 1387 TCATGA TfiI 1301 GAATC G^A[AT]TC 1556 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII