##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccII AccIII AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbrPI BcgI BciVI BclI
BfrI BglI BlnI BsaAI BsaI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BspMI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI FbaI FseI
HpaI KpnI KspI MluI MroI
MvnI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SwaI Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 2642 GACGTC GACGT^C
AclI 1326 AACGTT AA^CGTT
AflIII 2363 ACACGT A^C[GA][CT]GT
Alw44I 834 GTGCAC G^TGCAC
AlwNI 706 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1500 GGGCCC GGGCC^C
ApaLI 834 GTGCAC G^TGCAC
AviII 784 TGCGCA TGC^GCA
BamHI 2429 GGATCC G^GATCC
BanI 767 GGCGCC G^G[CT][GA]CC
BbeI 767 GGCGCC GGCGC^C
BbsI 969 GAAGAC GAAGAC(2/6)
BbvCI 526 CCTCAGC CCTCAGC(-5/-2)
BglII 2654 AGATCT A^GATCT
BpuAI 969 GAAGAC GAAGAC(2/6)
BsaBI 626 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 157 TGTACA T^GTACA
BspHI 1472 TCATGA T^CATGA
BsrBI 236 GAGCGG CCGCTC(-3/-3)
BsrGI 157 TGTACA T^GTACA
BstAPI 1047 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 1614 GACTTGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 345 CTCTTC CTCTTC(1/4)
EcoRV 630 GATATC GAT^ATC
EcoT22I 2038 ATGCAT ATGCA^T
FspI 784 TGCGCA TGC^GCA
HgaI 575 GACGC GACGC(5/10)
HindIII 1858 AAGCTT A^AGCTT
KasI 767 GGCGCC G^GCGCC
Ksp632I 345 CTCTTC CTCTTC(1/4)
MamI 626 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2401 CAATTG C^AATTG
MunI 2401 CAATTG C^AATTG
NarI 767 GGCGCC GG^CGCC
NsiI 2038 ATGCAT ATGCA^T
PflMI 2400 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1326 AACGTT AA^CGTT
RcaI 1472 TCATGA T^CATGA
SacI 718 GAGCTC GAGCT^C
SfoI 767 GGCGCC GGC^GCC
SmaI 355 CCCGGG CCC^GGG
SphI 909 GCATGC GCATG^C
SspBI 157 TGTACA T^GTACA
StuI 1165 AGGCCT AGG^CCT
TaqI 923 TCGA T^CGA
TthHB8I 923 TCGA T^CGA
Van91I 2400 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2567 TCTAGA T^CTAGA
XmaCI 355 CCCGGG C^CCGGG
XmaI 355 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 462 GTCTAC GT^[AC][GT]AC
2441 GTAGAC
AcyI 767 GGCGCC G[GA]^CG[CT]C
2642 GACGTC
AspHI 718 GAGCTC G[AT]GC[AT]^C
834 GTGCAC
BalI 310 TGGCCA TGG^CCA
394 TGGCCA
BlpI 278 GCTGAGC GC^T[ACGT]AGC
1428 GCTCAGC
BpmI 1718 CTCCAG CTGGAG(16/14)
1466 CTGGAG
Bpu1102I 278 GCTGAGC GC^T[ACGT]AGC
1428 GCTCAGC
BsaHI 767 GGCGCC G[GA]^CG[CT]C
2642 GACGTC
BsiHKAI 718 GAGCTC G[AT]GC[AT]^C
834 GTGCAC
BsmBI 608 CGTCTC CGTCTC(1/5)
1095 CGTCTC
BsmI 120 GAATGC GAATGC(1/-1)
16 GCATTC
BstEII 521 GGTCACC G^GT[ACGT]ACC
1945 GGTAACC
BstPI 521 GGTCACC G^GT[ACGT]ACC
1945 GGTAACC
CelII 278 GCTGAGC GC^T[ACGT]AGC
1428 GCTCAGC
EcoO65I 521 GGTCACC G^GT[ACGT]ACC
1945 GGTAACC
HaeII 563 GGCGCT [GA]GCGC^[CT]
767 GGCGCC
Hin1I 767 GGCGCC G[GA]^CG[CT]C
2642 GACGTC
MluNI 310 TGGCCA TGG^CCA
394 TGGCCA
MscI 310 TGGCCA TGG^CCA
394 TGGCCA
MspA1I 132 CCGCTG C[AC]G^C[GT]G
2341 CCGCTG
NcoI 892 CCATGG C^CATGG
2599 CCATGG
NspI 909 GCATGC [GA]CATG^[CT]
988 ACATGC
PpuMI 358 GGGACCT [GA]G^G[AT]CC[CT]
1226 GGGACCT
PstI 1510 CTGCAG CTGCA^G
1935 CTGCAG
SspI 2180 AATATT AAT^ATT
2725 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII