################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## Acc65I AccII AccIII AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbrPI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI FbaI FseI HpaI KpnI KspI MluI MroI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 2642 GACGTC GACGT^C AclI 1326 AACGTT AA^CGTT AflIII 2363 ACACGT A^C[GA][CT]GT Alw44I 834 GTGCAC G^TGCAC AlwNI 706 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1500 GGGCCC GGGCC^C ApaLI 834 GTGCAC G^TGCAC AviII 784 TGCGCA TGC^GCA BamHI 2429 GGATCC G^GATCC BanI 767 GGCGCC G^G[CT][GA]CC BbeI 767 GGCGCC GGCGC^C BbsI 969 GAAGAC GAAGAC(2/6) BbvCI 526 CCTCAGC CCTCAGC(-5/-2) BglII 2654 AGATCT A^GATCT BpuAI 969 GAAGAC GAAGAC(2/6) BsaBI 626 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 157 TGTACA T^GTACA BspHI 1472 TCATGA T^CATGA BsrBI 236 GAGCGG CCGCTC(-3/-3) BsrGI 157 TGTACA T^GTACA BstAPI 1047 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 1614 GACTTGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 345 CTCTTC CTCTTC(1/4) EcoRV 630 GATATC GAT^ATC EcoT22I 2038 ATGCAT ATGCA^T FspI 784 TGCGCA TGC^GCA HgaI 575 GACGC GACGC(5/10) HindIII 1858 AAGCTT A^AGCTT KasI 767 GGCGCC G^GCGCC Ksp632I 345 CTCTTC CTCTTC(1/4) MamI 626 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2401 CAATTG C^AATTG MunI 2401 CAATTG C^AATTG NarI 767 GGCGCC GG^CGCC NsiI 2038 ATGCAT ATGCA^T PflMI 2400 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1326 AACGTT AA^CGTT RcaI 1472 TCATGA T^CATGA SacI 718 GAGCTC GAGCT^C SfoI 767 GGCGCC GGC^GCC SmaI 355 CCCGGG CCC^GGG SphI 909 GCATGC GCATG^C SspBI 157 TGTACA T^GTACA StuI 1165 AGGCCT AGG^CCT TaqI 923 TCGA T^CGA TthHB8I 923 TCGA T^CGA Van91I 2400 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2567 TCTAGA T^CTAGA XmaCI 355 CCCGGG C^CCGGG XmaI 355 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 462 GTCTAC GT^[AC][GT]AC 2441 GTAGAC AcyI 767 GGCGCC G[GA]^CG[CT]C 2642 GACGTC AspHI 718 GAGCTC G[AT]GC[AT]^C 834 GTGCAC BalI 310 TGGCCA TGG^CCA 394 TGGCCA BlpI 278 GCTGAGC GC^T[ACGT]AGC 1428 GCTCAGC BpmI 1718 CTCCAG CTGGAG(16/14) 1466 CTGGAG Bpu1102I 278 GCTGAGC GC^T[ACGT]AGC 1428 GCTCAGC BsaHI 767 GGCGCC G[GA]^CG[CT]C 2642 GACGTC BsiHKAI 718 GAGCTC G[AT]GC[AT]^C 834 GTGCAC BsmBI 608 CGTCTC CGTCTC(1/5) 1095 CGTCTC BsmI 120 GAATGC GAATGC(1/-1) 16 GCATTC BstEII 521 GGTCACC G^GT[ACGT]ACC 1945 GGTAACC BstPI 521 GGTCACC G^GT[ACGT]ACC 1945 GGTAACC CelII 278 GCTGAGC GC^T[ACGT]AGC 1428 GCTCAGC EcoO65I 521 GGTCACC G^GT[ACGT]ACC 1945 GGTAACC HaeII 563 GGCGCT [GA]GCGC^[CT] 767 GGCGCC Hin1I 767 GGCGCC G[GA]^CG[CT]C 2642 GACGTC MluNI 310 TGGCCA TGG^CCA 394 TGGCCA MscI 310 TGGCCA TGG^CCA 394 TGGCCA MspA1I 132 CCGCTG C[AC]G^C[GT]G 2341 CCGCTG NcoI 892 CCATGG C^CATGG 2599 CCATGG NspI 909 GCATGC [GA]CATG^[CT] 988 ACATGC PpuMI 358 GGGACCT [GA]G^G[AT]CC[CT] 1226 GGGACCT PstI 1510 CTGCAG CTGCA^G 1935 CTGCAG SspI 2180 AATATT AAT^ATT 2725 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaI AvaII BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII