##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbrPI BciVI BclI BfrI
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspLU11I
BsrDI BsrFI BssHII Bst1107I BstBI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MluI MroI MvnI NaeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 926 GTCGAC GT^[AC][GT]AC
AcsI 2493 AAATTT [GA]^AATT[CT]
ApoI 2493 AAATTT [GA]^AATT[CT]
Asp700I 1906 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2060 TGGCCA TGG^CCA
BbeI 186 GGCGCC GGCGC^C
BbvCI 1300 GCTGAGG CCTCAGC(-5/-2)
BcgI 175 GCAGTGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1476 GCCAAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1229 AGATCT A^GATCT
BsaAI 286 TACGTG [CT]AC^GT[GA]
BsgI 1996 CTGCAC GTGCAG(16/14)
BsmBI 12 GAGACG CGTCTC(1/5)
Bsp1407I 1201 TGTACA T^GTACA
BspMI 2010 ACCTGC ACCTGC(4/8)
BsrBI 869 GAGCGG CCGCTC(-3/-3)
BsrGI 1201 TGTACA T^GTACA
BssSI 340 CACGAG CACGAG(-5/-1)
DraIII 1850 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 620 CCATGG C^C[GA][CT]GG
EcoT22I 1965 ATGCAT ATGCA^T
HincII 926 GTCGAC GT[CT]^[GA]AC
HindII 926 GTCGAC GT[CT]^[GA]AC
KasI 186 GGCGCC G^GCGCC
MfeI 1270 CAATTG C^AATTG
MluNI 2060 TGGCCA TGG^CCA
MscI 2060 TGGCCA TGG^CCA
MseI 476 TTAA T^TAA
MunI 1270 CAATTG C^AATTG
NarI 186 GGCGCC GG^CGCC
NcoI 620 CCATGG C^CATGG
NsiI 1965 ATGCAT ATGCA^T
PaeR7I 69 CTCGAG C^TCGAG
SalI 926 GTCGAC G^TCGAC
SapI 387 GCTCTTC GCTCTTC(1/4)
SfoI 186 GGCGCC GGC^GCC
SmaI 121 CCCGGG CCC^GGG
SspBI 1201 TGTACA T^GTACA
SspI 2474 AATATT AAT^ATT
StuI 1869 AGGCCT AGG^CCT
TfiI 594 GATTC G^A[AT]TC
Tru9I 476 TTAA T^TAA
XhoI 69 CTCGAG C^TCGAG
XmaCI 121 CCCGGG C^CCGGG
XmaI 121 CCCGGG C^CCGGG
XmnI 1906 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 186 GGCGCC G[GA]^CG[CT]C
513 GGCGTC
AlwI 1355 GGATC GGATC(4/5)
2215 GGATC
AspI 481 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2386 GACCCTGTC
BsaHI 186 GGCGCC G[GA]^CG[CT]C
513 GGCGTC
BsaI 1113 GAGACC GGTCTC(1/5)
1374 GAGACC
BspHI 83 TCATGA T^CATGA
779 TCATGA
BstAPI 1677 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2224 GCAGAGTGTGC
CfoI 187 GCGC GCG^C
1955 GCGC
HaeII 186 GGCGCC [GA]GCGC^[CT]
1954 GGCGCT
HhaI 187 GCGC GCG^C
1955 GCGC
Hin1I 186 GGCGCC G[GA]^CG[CT]C
513 GGCGTC
HinP1I 187 GCGC G^CGC
1955 GCGC
MslI 633 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
663 CACATCTGTG
NdeI 1205 CATATG CA^TATG
1769 CATATG
PflFI 481 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2386 GACCCTGTC
PflMI 423 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAGCTAATGG
RcaI 83 TCATGA T^CATGA
779 TCATGA
SacI 1700 GAGCTC GAGCT^C
2187 GAGCTC
SmlI 69 CTCGAG C^T[CT][GA]AG
2159 CTTGAG
TaqI 70 TCGA T^CGA
927 TCGA
Tth111I 481 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2386 GACCCTGTC
TthHB8I 70 TCGA T^CGA
927 TCGA
Van91I 423 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAGCTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I Tsp509I TspRI
XhoII