################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflIII AgeI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AviII BbrPI BcgI BclI BglI BglII BsaAI BsaBI BsiEI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MaeII MamI MluI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 1663 TCCGGA T^CCGGA AcyI 133 GGCGCC G[GA]^CG[CT]C AflII 1138 CTTAAG C^TTAAG AhdI 321 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2005 CCTCAGG CC^T[ACGT]AGG Aor51HI 73 AGCGCT AGC^GCT ApaI 2317 GGGCCC GGGCC^C AspEI 321 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2792 GTGCTC G[AT]GC[AT]^C AspI 459 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2446 CCTAGG C^CTAGG BamHI 1012 GGATCC G^GATCC BbeI 133 GGCGCC GGCGC^C BbvCI 781 GCTGAGG CCTCAGC(-5/-2) BciVI 1985 GGATAC GTATCC(6/5) BfrI 1138 CTTAAG C^TTAAG BlnI 2446 CCTAGG C^CTAGG BlpI 76 GCTTAGC GC^T[ACGT]AGC Bpu1102I 76 GCTTAGC GC^T[ACGT]AGC BsaHI 133 GGCGCC G[GA]^CG[CT]C BseAI 1663 TCCGGA T^CCGGA BsiHKAI 2792 GTGCTC G[AT]GC[AT]^C BsiWI 720 CGTACG C^GTACG BspEI 1663 TCCGGA T^CCGGA BstAPI 1297 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2005 CCTCAGG CC^T[ACGT]AGG CelII 76 GCTTAGC GC^T[ACGT]AGC Eam1105I 321 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 73 AGCGCT AGC^GCT Eco81I 2005 CCTCAGG CC^T[ACGT]AGG Hin1I 133 GGCGCC G[GA]^CG[CT]C KasI 133 GGCGCC G^GCGCC KspI 2114 CCGCGG CCGC^GG MfeI 954 CAATTG C^AATTG MroI 1663 TCCGGA T^CCGGA MseI 1139 TTAA T^TAA MunI 954 CAATTG C^AATTG NaeI 146 GCCGGC GCC^GGC NarI 133 GGCGCC GG^CGCC NgoMIV 146 GCCGGC G^CCGGC NspI 2495 GCATGC [GA]CATG^[CT] PflFI 459 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 210 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 2114 CCGCGG CCGC^GG SfoI 133 GGCGCC GGC^GCC SphI 2495 GCATGC GCATG^C TaqI 269 TCGA T^CGA Tru9I 1139 TTAA T^TAA Tth111I 459 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 269 TCGA T^CGA Van91I 210 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2238 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 17 CGCG CG^CG 2115 CGCG Asp700I 1208 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2230 GAAGAACTTC AvaI 129 CCCGGG C^[CT]CG[GA]G 2534 CCCGGG BsaWI 368 ACCGGA [AT]^CCGG[AT] 1663 TCCGGA BsoBI 129 CCCGGG C^[CT]CG[GA]G 2534 CCCGGG BsrBI 522 CCGCTC CCGCTC(-3/-3) 873 CCGCTC BsrDI 777 CATTGC GCAATG(2/0) 1937 GCAATG BsrFI 146 GCCGGC [GA]^CCGG[CT] 545 ACCGGC BstUI 17 CGCG CG^CG 2115 CGCG Cfr10I 146 GCCGGC [GA]^CCGG[CT] 545 ACCGGC DrdI 1953 GACCAATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2691 GACAGTCTTGTC EarI 1212 CTCTTC CTCTTC(1/4) 2082 CTCTTC HaeII 73 AGCGCT [GA]GCGC^[CT] 133 GGCGCC Ksp632I 1212 CTCTTC CTCTTC(1/4) 2082 CTCTTC MslI 1048 CATATTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2669 CATGGTGATG MvnI 17 CGCG CG^CG 2115 CGCG NcoI 215 CCATGG C^CATGG 2668 CCATGG ScaI 284 AGTACT AGT^ACT 2572 AGTACT SexAI 1655 ACCTGGT A^CC[AT]GGT 2522 ACCTGGT SmaI 129 CCCGGG CCC^GGG 2534 CCCGGG StuI 1637 AGGCCT AGG^CCT 2765 AGGCCT Tsp509I 955 AATT ^AATT 1257 AATT XmaCI 129 CCCGGG C^CCGGG 2534 CCCGGG XmaI 129 CCCGGG C^CCGGG 2534 CCCGGG XmnI 1208 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2230 GAAGAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI TseI Tsp45I TspRI XcmI XhoII