##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflIII AgeI Alw44I ApaLI ApoI
AscI AseI AsnI Asp718I AviII
BbrPI BcgI BclI BglI BglII
BsaAI BsaBI BsiEI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EagI EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MaeII
MamI MluI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 1663 TCCGGA T^CCGGA
AcyI 133 GGCGCC G[GA]^CG[CT]C
AflII 1138 CTTAAG C^TTAAG
AhdI 321 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2005 CCTCAGG CC^T[ACGT]AGG
Aor51HI 73 AGCGCT AGC^GCT
ApaI 2317 GGGCCC GGGCC^C
AspEI 321 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2792 GTGCTC G[AT]GC[AT]^C
AspI 459 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2446 CCTAGG C^CTAGG
BamHI 1012 GGATCC G^GATCC
BbeI 133 GGCGCC GGCGC^C
BbvCI 781 GCTGAGG CCTCAGC(-5/-2)
BciVI 1985 GGATAC GTATCC(6/5)
BfrI 1138 CTTAAG C^TTAAG
BlnI 2446 CCTAGG C^CTAGG
BlpI 76 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 76 GCTTAGC GC^T[ACGT]AGC
BsaHI 133 GGCGCC G[GA]^CG[CT]C
BseAI 1663 TCCGGA T^CCGGA
BsiHKAI 2792 GTGCTC G[AT]GC[AT]^C
BsiWI 720 CGTACG C^GTACG
BspEI 1663 TCCGGA T^CCGGA
BstAPI 1297 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2005 CCTCAGG CC^T[ACGT]AGG
CelII 76 GCTTAGC GC^T[ACGT]AGC
Eam1105I 321 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 73 AGCGCT AGC^GCT
Eco81I 2005 CCTCAGG CC^T[ACGT]AGG
Hin1I 133 GGCGCC G[GA]^CG[CT]C
KasI 133 GGCGCC G^GCGCC
KspI 2114 CCGCGG CCGC^GG
MfeI 954 CAATTG C^AATTG
MroI 1663 TCCGGA T^CCGGA
MseI 1139 TTAA T^TAA
MunI 954 CAATTG C^AATTG
NaeI 146 GCCGGC GCC^GGC
NarI 133 GGCGCC GG^CGCC
NgoMIV 146 GCCGGC G^CCGGC
NspI 2495 GCATGC [GA]CATG^[CT]
PflFI 459 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 210 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 2114 CCGCGG CCGC^GG
SfoI 133 GGCGCC GGC^GCC
SphI 2495 GCATGC GCATG^C
TaqI 269 TCGA T^CGA
Tru9I 1139 TTAA T^TAA
Tth111I 459 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 269 TCGA T^CGA
Van91I 210 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2238 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 17 CGCG CG^CG
2115 CGCG
Asp700I 1208 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2230 GAAGAACTTC
AvaI 129 CCCGGG C^[CT]CG[GA]G
2534 CCCGGG
BsaWI 368 ACCGGA [AT]^CCGG[AT]
1663 TCCGGA
BsoBI 129 CCCGGG C^[CT]CG[GA]G
2534 CCCGGG
BsrBI 522 CCGCTC CCGCTC(-3/-3)
873 CCGCTC
BsrDI 777 CATTGC GCAATG(2/0)
1937 GCAATG
BsrFI 146 GCCGGC [GA]^CCGG[CT]
545 ACCGGC
BstUI 17 CGCG CG^CG
2115 CGCG
Cfr10I 146 GCCGGC [GA]^CCGG[CT]
545 ACCGGC
DrdI 1953 GACCAATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2691 GACAGTCTTGTC
EarI 1212 CTCTTC CTCTTC(1/4)
2082 CTCTTC
HaeII 73 AGCGCT [GA]GCGC^[CT]
133 GGCGCC
Ksp632I 1212 CTCTTC CTCTTC(1/4)
2082 CTCTTC
MslI 1048 CATATTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2669 CATGGTGATG
MvnI 17 CGCG CG^CG
2115 CGCG
NcoI 215 CCATGG C^CATGG
2668 CCATGG
ScaI 284 AGTACT AGT^ACT
2572 AGTACT
SexAI 1655 ACCTGGT A^CC[AT]GGT
2522 ACCTGGT
SmaI 129 CCCGGG CCC^GGG
2534 CCCGGG
StuI 1637 AGGCCT AGG^CCT
2765 AGGCCT
Tsp509I 955 AATT ^AATT
1257 AATT
XmaCI 129 CCCGGG C^CCGGG
2534 CCCGGG
XmaI 129 CCCGGG C^CCGGG
2534 CCCGGG
XmnI 1208 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2230 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI TseI Tsp45I TspRI
XcmI XhoII