################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AclI AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII BamHI BbsI BcgI BglII BpuAI BsaBI BsiWI BsmBI BsmI BspDI BspHI BsrBI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HincII HindII HpaI MamI MfeI MflI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 267 GACGTC GACGT^C Acc65I 326 GGTACC G^GTACC AcsI 353 AAATTC [GA]^AATT[CT] AflII 1723 CTTAAG C^TTAAG AflIII 2409 ACATGT A^C[GA][CT]GT ApoI 353 AAATTC [GA]^AATT[CT] Asp718I 326 GGTACC G^GTACC AvrII 1676 CCTAGG C^CTAGG BalI 496 TGGCCA TGG^CCA BbrPI 647 CACGTG CAC^GTG BbvCI 1402 GCTGAGG CCTCAGC(-5/-2) BciVI 1843 GTATCC GTATCC(6/5) BclI 1373 TGATCA T^GATCA BfaI 1677 CTAG C^TAG BfrI 1723 CTTAAG C^TTAAG BlnI 1676 CCTAGG C^CTAGG BlpI 2091 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2091 GCTGAGC GC^T[ACGT]AGC BsaAI 647 CACGTG [CT]AC^GT[GA] BsiEI 457 CGACCG CG[GA][CT]^CG Bsp1407I 2501 TGTACA T^GTACA BspLU11I 2409 ACATGT A^CATGT BsrDI 714 GCAATG GCAATG(2/0) BsrFI 184 GCCGGC [GA]^CCGG[CT] BsrGI 2501 TGTACA T^GTACA BstAPI 1389 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 429 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2091 GCTGAGC GC^T[ACGT]AGC Cfr10I 184 GCCGGC [GA]^CCGG[CT] EcoNI 958 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1373 TGATCA T^GATCA HindIII 2294 AAGCTT A^AGCTT KpnI 326 GGTACC GGTAC^C MaeI 1677 CTAG C^TAG MluNI 496 TGGCCA TGG^CCA MscI 496 TGGCCA TGG^CCA MslI 1259 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 184 GCCGGC GCC^GGC NgoMIV 184 GCCGGC G^CCGGC NspI 2409 ACATGT [GA]CATG^[CT] PflMI 2323 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 647 CACGTG CAC^GTG PmlI 647 CACGTG CAC^GTG SacI 1202 GAGCTC GAGCT^C SfcI 566 CTACAG C^T[GA][CT]AG SfiI 622 GGCCGCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 2501 TGTACA T^GTACA Van91I 2323 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 2069 GTAGAC GT^[AC][GT]AC 2196 GTAGAC AccIII 319 TCCGGA T^CCGGA 1282 TCCGGA AfaI 327 GTAC GT^AC 2502 GTAC Alw44I 301 GTGCAC G^TGCAC 726 GTGCAC AlwNI 1224 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG 2324 CAGGACCTG ApaLI 301 GTGCAC G^TGCAC 726 GTGCAC AvaI 533 CTCGGG C^[CT]CG[GA]G 1440 CTCGGG BanII 1202 GAGCTC G[GA]GC[CT]^C 1335 GGGCTC BbeI 545 GGCGCC GGCGC^C 1327 GGCGCC BsaI 1168 GAGACC GGTCTC(1/5) 1657 GAGACC BseAI 319 TCCGGA T^CCGGA 1282 TCCGGA BsgI 110 CTGCAC GTGCAG(16/14) 672 CTGCAC BsoBI 533 CTCGGG C^[CT]CG[GA]G 1440 CTCGGG BspEI 319 TCCGGA T^CCGGA 1282 TCCGGA BspMI 957 ACCTGC ACCTGC(4/8) 1926 ACCTGC BssHII 466 GCGCGC G^CGCGC 468 GCGCGC DraI 1628 TTTAAA TTT^AAA 2373 TTTAAA FauI 686 CCCGC CCCGC(4/6) 1820 CCCGC KasI 545 GGCGCC G^GCGCC 1327 GGCGCC KspI 624 CCGCGG CCGC^GG 687 CCGCGG MroI 319 TCCGGA T^CCGGA 1282 TCCGGA NarI 545 GGCGCC GG^CGCC 1327 GGCGCC NcoI 750 CCATGG C^CATGG 1365 CCATGG PpuMI 2309 AGGTCCC [GA]G^G[AT]CC[CT] 2325 AGGACCT PvuII 40 CAGCTG CAG^CTG 500 CAGCTG RsaI 327 GTAC GT^AC 2502 GTAC SacII 624 CCGCGG CCGC^GG 687 CCGCGG SfoI 545 GGCGCC GGC^GCC 1327 GGCGCC StuI 1187 AGGCCT AGG^CCT 1872 AGGCCT TaqI 294 TCGA T^CGA 2126 TCGA Tsp45I 1216 GTCAC ^GT[GC]AC 2223 GTGAC TthHB8I 294 TCGA T^CGA 2126 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AocI AspHI AvaII BanI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp509I TspRI XcmI