##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AclI AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AspI AviII BamHI BbsI BcgI
BglII BpuAI BsaBI BsiWI BsmBI
BsmI BspDI BspHI BsrBI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI HincII HindII HpaI
MamI MfeI MflI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 267 GACGTC GACGT^C
Acc65I 326 GGTACC G^GTACC
AcsI 353 AAATTC [GA]^AATT[CT]
AflII 1723 CTTAAG C^TTAAG
AflIII 2409 ACATGT A^C[GA][CT]GT
ApoI 353 AAATTC [GA]^AATT[CT]
Asp718I 326 GGTACC G^GTACC
AvrII 1676 CCTAGG C^CTAGG
BalI 496 TGGCCA TGG^CCA
BbrPI 647 CACGTG CAC^GTG
BbvCI 1402 GCTGAGG CCTCAGC(-5/-2)
BciVI 1843 GTATCC GTATCC(6/5)
BclI 1373 TGATCA T^GATCA
BfaI 1677 CTAG C^TAG
BfrI 1723 CTTAAG C^TTAAG
BlnI 1676 CCTAGG C^CTAGG
BlpI 2091 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2091 GCTGAGC GC^T[ACGT]AGC
BsaAI 647 CACGTG [CT]AC^GT[GA]
BsiEI 457 CGACCG CG[GA][CT]^CG
Bsp1407I 2501 TGTACA T^GTACA
BspLU11I 2409 ACATGT A^CATGT
BsrDI 714 GCAATG GCAATG(2/0)
BsrFI 184 GCCGGC [GA]^CCGG[CT]
BsrGI 2501 TGTACA T^GTACA
BstAPI 1389 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 429 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2091 GCTGAGC GC^T[ACGT]AGC
Cfr10I 184 GCCGGC [GA]^CCGG[CT]
EcoNI 958 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1373 TGATCA T^GATCA
HindIII 2294 AAGCTT A^AGCTT
KpnI 326 GGTACC GGTAC^C
MaeI 1677 CTAG C^TAG
MluNI 496 TGGCCA TGG^CCA
MscI 496 TGGCCA TGG^CCA
MslI 1259 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 184 GCCGGC GCC^GGC
NgoMIV 184 GCCGGC G^CCGGC
NspI 2409 ACATGT [GA]CATG^[CT]
PflMI 2323 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 647 CACGTG CAC^GTG
PmlI 647 CACGTG CAC^GTG
SacI 1202 GAGCTC GAGCT^C
SfcI 566 CTACAG C^T[GA][CT]AG
SfiI 622 GGCCGCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 2501 TGTACA T^GTACA
Van91I 2323 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2069 GTAGAC GT^[AC][GT]AC
2196 GTAGAC
AccIII 319 TCCGGA T^CCGGA
1282 TCCGGA
AfaI 327 GTAC GT^AC
2502 GTAC
Alw44I 301 GTGCAC G^TGCAC
726 GTGCAC
AlwNI 1224 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
2324 CAGGACCTG
ApaLI 301 GTGCAC G^TGCAC
726 GTGCAC
AvaI 533 CTCGGG C^[CT]CG[GA]G
1440 CTCGGG
BanII 1202 GAGCTC G[GA]GC[CT]^C
1335 GGGCTC
BbeI 545 GGCGCC GGCGC^C
1327 GGCGCC
BsaI 1168 GAGACC GGTCTC(1/5)
1657 GAGACC
BseAI 319 TCCGGA T^CCGGA
1282 TCCGGA
BsgI 110 CTGCAC GTGCAG(16/14)
672 CTGCAC
BsoBI 533 CTCGGG C^[CT]CG[GA]G
1440 CTCGGG
BspEI 319 TCCGGA T^CCGGA
1282 TCCGGA
BspMI 957 ACCTGC ACCTGC(4/8)
1926 ACCTGC
BssHII 466 GCGCGC G^CGCGC
468 GCGCGC
DraI 1628 TTTAAA TTT^AAA
2373 TTTAAA
FauI 686 CCCGC CCCGC(4/6)
1820 CCCGC
KasI 545 GGCGCC G^GCGCC
1327 GGCGCC
KspI 624 CCGCGG CCGC^GG
687 CCGCGG
MroI 319 TCCGGA T^CCGGA
1282 TCCGGA
NarI 545 GGCGCC GG^CGCC
1327 GGCGCC
NcoI 750 CCATGG C^CATGG
1365 CCATGG
PpuMI 2309 AGGTCCC [GA]G^G[AT]CC[CT]
2325 AGGACCT
PvuII 40 CAGCTG CAG^CTG
500 CAGCTG
RsaI 327 GTAC GT^AC
2502 GTAC
SacII 624 CCGCGG CCGC^GG
687 CCGCGG
SfoI 545 GGCGCC GGC^GCC
1327 GGCGCC
StuI 1187 AGGCCT AGG^CCT
1872 AGGCCT
TaqI 294 TCGA T^CGA
2126 TCGA
Tsp45I 1216 GTCAC ^GT[GC]AC
2223 GTGAC
TthHB8I 294 TCGA T^CGA
2126 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AocI AspHI AvaII BanI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TfiI Tru9I
TseI Tsp509I TspRI XcmI