################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AviII BalI BbeI BbrPI BbvCI BciVI BcnI BglII BlpI Bpu1102I BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MamI MfeI MluNI MroI MscI MunI NaeI NarI NciI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 1899 GAATTT [GA]^AATT[CT] AcyI 205 GGCGTC G[GA]^CG[CT]C AflII 2220 CTTAAG C^TTAAG Alw44I 2203 GTGCAC G^TGCAC ApaLI 2203 GTGCAC G^TGCAC ApoI 1899 GAATTT [GA]^AATT[CT] AspI 2102 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1414 CCTAGG C^CTAGG BanII 1224 GGGCTC G[GA]GC[CT]^C BclI 607 TGATCA T^GATCA BfrI 2220 CTTAAG C^TTAAG BglI 43 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1414 CCTAGG C^CTAGG BsaHI 205 GGCGTC G[GA]^CG[CT]C BspHI 595 TCATGA T^CATGA BspMI 881 ACCTGC ACCTGC(4/8) BsrBI 2041 GAGCGG CCGCTC(-3/-3) BssSI 1057 CACGAG CACGAG(-5/-1) BstAPI 1575 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1135 TTCGAA TT^CGAA DraI 1372 TTTAAA TTT^AAA EaeI 502 CGGCCA [CT]^GGCC[GA] EcoNI 1386 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1544 ATGCAT ATGCA^T FbaI 607 TGATCA T^GATCA HapII 203 CCGG C^CGG Hin1I 205 GGCGTC G[GA]^CG[CT]C HindIII 140 AAGCTT A^AGCTT HpaII 203 CCGG C^CGG MluI 823 ACGCGT A^CGCGT MspA1I 320 CAGCGG C[AC]G^C[GT]G MspI 203 CCGG C^CGG NcoI 901 CCATGG C^CATGG NdeI 2431 CATATG CA^TATG NsiI 1544 ATGCAT ATGCA^T NspI 1542 ACATGC [GA]CATG^[CT] NspV 1135 TTCGAA TT^CGAA PflFI 2102 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 844 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 595 TCATGA T^CATGA ScaI 1442 AGTACT AGT^ACT SfuI 1135 TTCGAA TT^CGAA SnaBI 245 TACGTA TAC^GTA Tth111I 2102 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 844 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1189 TCTAGA T^CTAGA XcmI 37 CCAGCAGCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 713 GTAC GT^AC 1443 GTAC AflIII 823 ACGCGT A^C[GA][CT]GT 1669 ACGTGT AseI 1914 ATTAAT AT^TAAT 2526 ATTAAT AsnI 1914 ATTAAT AT^TAAT 2526 ATTAAT AvaI 806 CCCGAG C^[CT]CG[GA]G 1748 CCCGAG BamHI 266 GGATCC G^GATCC 905 GGATCC BanI 1011 GGTGCC G^G[CT][GA]CC 1504 GGTGCC BcgI 15 CGATGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1127 GCAACCACTTCG BsaAI 245 TACGTA [CT]AC^GT[GA] 1668 TACGTG BsmI 1193 GAATGC GAATGC(1/-1) 369 GCATTC BsoBI 806 CCCGAG C^[CT]CG[GA]G 1748 CCCGAG BsrDI 1309 CATTGC GCAATG(2/0) 166 GCAATG DsaI 697 CCACGG C^C[GA][CT]GG 901 CCATGG EarI 512 CTCTTC CTCTTC(1/4) 774 GAAGAG HgaI 206 GCGTC GACGC(5/10) 825 GCGTC Ksp632I 512 CTCTTC CTCTTC(1/4) 774 GAAGAG MaeII 246 ACGT A^CGT 1669 ACGT MslI 215 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 350 CATCGGGATG PshBI 1914 ATTAAT AT^TAAT 2526 ATTAAT PstI 750 CTGCAG CTGCA^G 969 CTGCAG RsaI 713 GTAC GT^AC 1443 GTAC SfcI 750 CTGCAG C^T[GA][CT]AG 969 CTGCAG SmlI 1789 CTCAAG C^T[CT][GA]AG 2220 CTTAAG StuI 1256 AGGCCT AGG^CCT 1449 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AspHI AvaII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PpuMI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII