##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AviII
BalI BbeI BbrPI BbvCI BciVI
BcnI BglII BlpI Bpu1102I BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FseI
FspI HaeII HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluNI MroI MscI MunI NaeI
NarI NciI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SspI SwaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcsI 1899 GAATTT [GA]^AATT[CT]
AcyI 205 GGCGTC G[GA]^CG[CT]C
AflII 2220 CTTAAG C^TTAAG
Alw44I 2203 GTGCAC G^TGCAC
ApaLI 2203 GTGCAC G^TGCAC
ApoI 1899 GAATTT [GA]^AATT[CT]
AspI 2102 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1414 CCTAGG C^CTAGG
BanII 1224 GGGCTC G[GA]GC[CT]^C
BclI 607 TGATCA T^GATCA
BfrI 2220 CTTAAG C^TTAAG
BglI 43 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1414 CCTAGG C^CTAGG
BsaHI 205 GGCGTC G[GA]^CG[CT]C
BspHI 595 TCATGA T^CATGA
BspMI 881 ACCTGC ACCTGC(4/8)
BsrBI 2041 GAGCGG CCGCTC(-3/-3)
BssSI 1057 CACGAG CACGAG(-5/-1)
BstAPI 1575 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1135 TTCGAA TT^CGAA
DraI 1372 TTTAAA TTT^AAA
EaeI 502 CGGCCA [CT]^GGCC[GA]
EcoNI 1386 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1544 ATGCAT ATGCA^T
FbaI 607 TGATCA T^GATCA
HapII 203 CCGG C^CGG
Hin1I 205 GGCGTC G[GA]^CG[CT]C
HindIII 140 AAGCTT A^AGCTT
HpaII 203 CCGG C^CGG
MluI 823 ACGCGT A^CGCGT
MspA1I 320 CAGCGG C[AC]G^C[GT]G
MspI 203 CCGG C^CGG
NcoI 901 CCATGG C^CATGG
NdeI 2431 CATATG CA^TATG
NsiI 1544 ATGCAT ATGCA^T
NspI 1542 ACATGC [GA]CATG^[CT]
NspV 1135 TTCGAA TT^CGAA
PflFI 2102 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 844 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 595 TCATGA T^CATGA
ScaI 1442 AGTACT AGT^ACT
SfuI 1135 TTCGAA TT^CGAA
SnaBI 245 TACGTA TAC^GTA
Tth111I 2102 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 844 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1189 TCTAGA T^CTAGA
XcmI 37 CCAGCAGCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 713 GTAC GT^AC
1443 GTAC
AflIII 823 ACGCGT A^C[GA][CT]GT
1669 ACGTGT
AseI 1914 ATTAAT AT^TAAT
2526 ATTAAT
AsnI 1914 ATTAAT AT^TAAT
2526 ATTAAT
AvaI 806 CCCGAG C^[CT]CG[GA]G
1748 CCCGAG
BamHI 266 GGATCC G^GATCC
905 GGATCC
BanI 1011 GGTGCC G^G[CT][GA]CC
1504 GGTGCC
BcgI 15 CGATGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1127 GCAACCACTTCG
BsaAI 245 TACGTA [CT]AC^GT[GA]
1668 TACGTG
BsmI 1193 GAATGC GAATGC(1/-1)
369 GCATTC
BsoBI 806 CCCGAG C^[CT]CG[GA]G
1748 CCCGAG
BsrDI 1309 CATTGC GCAATG(2/0)
166 GCAATG
DsaI 697 CCACGG C^C[GA][CT]GG
901 CCATGG
EarI 512 CTCTTC CTCTTC(1/4)
774 GAAGAG
HgaI 206 GCGTC GACGC(5/10)
825 GCGTC
Ksp632I 512 CTCTTC CTCTTC(1/4)
774 GAAGAG
MaeII 246 ACGT A^CGT
1669 ACGT
MslI 215 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
350 CATCGGGATG
PshBI 1914 ATTAAT AT^TAAT
2526 ATTAAT
PstI 750 CTGCAG CTGCA^G
969 CTGCAG
RsaI 713 GTAC GT^AC
1443 GTAC
SfcI 750 CTGCAG C^T[GA][CT]AG
969 CTGCAG
SmlI 1789 CTCAAG C^T[CT][GA]AG
2220 CTTAAG
StuI 1256 AGGCCT AGG^CCT
1449 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PpuMI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII