##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BsgI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
Bst1107I BstBI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV FauI FseI
FspI HgaI HpaI KpnI KspI
MamI MluI MluNI MroI MscI
MvnI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcsI 1115 AAATTT [GA]^AATT[CT]
AcyI 1214 GGCGCC G[GA]^CG[CT]C
AgeI 1531 ACCGGT A^CCGGT
AhdI 1125 GACGGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1115 AAATTT [GA]^AATT[CT]
AspEI 1125 GACGGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1142 GTGCTC G[AT]GC[AT]^C
BamHI 1477 GGATCC G^GATCC
BanI 1214 GGCGCC G^G[CT][GA]CC
BbeI 1214 GGCGCC GGCGC^C
BbsI 675 GAAGAC GAAGAC(2/6)
BclI 78 TGATCA T^GATCA
BglI 1938 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 675 GAAGAC GAAGAC(2/6)
BsaHI 1214 GGCGCC G[GA]^CG[CT]C
BsaI 314 GAGACC GGTCTC(1/5)
BsiEI 1895 CGATCG CG[GA][CT]^CG
BsiHKAI 1142 GTGCTC G[AT]GC[AT]^C
BspLU11I 2264 ACATGT A^CATGT
BsrBI 610 GAGCGG CCGCTC(-3/-3)
BstAPI 1168 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1405 GGTCACC G^GT[ACGT]ACC
BstPI 1405 GGTCACC G^GT[ACGT]ACC
BstYI 1477 GGATCC [GA]^GATC[CT]
DraI 1795 TTTAAA TTT^AAA
EaeI 241 TGGCCG [CT]^GGCC[GA]
Eam1105I 1125 GACGGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2218 CTCTTC CTCTTC(1/4)
EcoO65I 1405 GGTCACC G^GT[ACGT]ACC
EcoT22I 710 ATGCAT ATGCA^T
FbaI 78 TGATCA T^GATCA
Hin1I 1214 GGCGCC G[GA]^CG[CT]C
KasI 1214 GGCGCC G^GCGCC
Ksp632I 2218 CTCTTC CTCTTC(1/4)
MfeI 1823 CAATTG C^AATTG
MflI 1477 GGATCC [GA]^GATC[CT]
MspA1I 198 CAGCTG C[AC]G^C[GT]G
MunI 1823 CAATTG C^AATTG
NaeI 243 GCCGGC GCC^GGC
NarI 1214 GGCGCC GG^CGCC
NcoI 875 CCATGG C^CATGG
NgoMIV 243 GCCGGC G^CCGGC
NsiI 710 ATGCAT ATGCA^T
PflMI 2044 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1531 ACCGGT A^CCGGT
PvuI 1895 CGATCG CGAT^CG
PvuII 198 CAGCTG CAG^CTG
SfoI 1214 GGCGCC GGC^GCC
SphI 1704 GCATGC GCATG^C
StuI 2046 AGGCCT AGG^CCT
Van91I 2044 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1477 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 2249 ACACGT A^C[GA][CT]GT
2264 ACATGT
AlwI 1478 GATCC GGATC(4/5)
1477 GGATC
BbrPI 1310 CACGTG CAC^GTG
2250 CACGTG
BcnI 781 CCGGG CC^[GC]GG
1982 CCGGG
BpmI 471 CTCCAG CTGGAG(16/14)
1203 CTGGAG
BsaWI 1110 ACCGGA [AT]^CCGG[AT]
1531 ACCGGT
BseRI 848 CTCCTC GAGGAG(10/8)
1697 CTCCTC
BsmI 2236 GAATGC GAATGC(1/-1)
2102 GCATTC
BsrDI 447 GCAATG GCAATG(2/0)
1807 GCAATG
BsrFI 243 GCCGGC [GA]^CCGG[CT]
1531 ACCGGT
BssSI 26 CTCGTG CACGAG(-5/-1)
1917 CTCGTG
CfoI 754 GCGC GCG^C
1215 GCGC
Cfr10I 243 GCCGGC [GA]^CCGG[CT]
1531 ACCGGT
DraII 910 GGGTCCC [GA]G^G[ACGT]CC[CT]
1294 GGGTCCT
DsaI 412 CCGTGG C^C[GA][CT]GG
875 CCATGG
EcoO109I 910 GGGTCCC [GA]G^G[ACGT]CC[CT]
1294 GGGTCCT
HaeII 753 AGCGCC [GA]GCGC^[CT]
1214 GGCGCC
HhaI 754 GCGC GCG^C
1215 GCGC
HinP1I 754 GCGC G^CGC
1215 GCGC
HincII 308 GTCAAC GT[CT]^[GA]AC
821 GTTGAC
HindII 308 GTCAAC GT[CT]^[GA]AC
821 GTTGAC
NciI 781 CCGGG CC^[GC]GG
1982 CCGGG
PleI 1952 GACTC GAGTC(4/5)
264 GAGTC
PmaCI 1310 CACGTG CAC^GTG
2250 CACGTG
PmlI 1310 CACGTG CAC^GTG
2250 CACGTG
PpuMI 910 GGGTCCC [GA]G^G[AT]CC[CT]
1294 GGGTCCT
SmlI 1713 CTTGAG C^T[CT][GA]AG
2013 CTTGAG
TaqI 420 TCGA T^CGA
1894 TCGA
TthHB8I 420 TCGA T^CGA
1894 TCGA
XcmI 1183 CCACCCCAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2160 CCAGCACCTACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccI AciI AfaI AluI AvaI
AvaII BanII BbvI BfaI BmyI
BsaAI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraIII EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI