##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII BamHI BbrPI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BsaAI BsaBI BseAI BsiEI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BstAPI BstBI
CelII Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KspI
MamI MfeI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tth111I TthHB8I Van91I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 872 GTATAC GT^[AC][GT]AC
AcsI 317 GAATTC [GA]^AATT[CT]
AcyI 245 GGCGCC G[GA]^CG[CT]C
AflIII 1194 ACGCGT A^C[GA][CT]GT
ApaI 501 GGGCCC GGGCC^C
ApoI 317 GAATTC [GA]^AATT[CT]
AvrII 512 CCTAGG C^CTAGG
BalI 200 TGGCCA TGG^CCA
BbeI 245 GGCGCC GGCGC^C
BbsI 115 GTCTTC GAAGAC(2/6)
BlnI 512 CCTAGG C^CTAGG
BpuAI 115 GTCTTC GAAGAC(2/6)
BsaHI 245 GGCGCC G[GA]^CG[CT]C
BsaWI 1175 TCCGGT [AT]^CCGG[AT]
BsiWI 1197 CGTACG C^GTACG
BsmBI 1284 CGTCTC CGTCTC(1/5)
BssSI 1147 CTCGTG CACGAG(-5/-1)
Bst1107I 872 GTATAC GTA^TAC
BstEII 1811 GGTTACC G^GT[ACGT]ACC
BstPI 1811 GGTTACC G^GT[ACGT]ACC
BstYI 1142 AGATCC [GA]^GATC[CT]
BstZ17I 872 GTATAC GTA^TAC
CfoI 246 GCGC GCG^C
DraI 2651 TTTAAA TTT^AAA
DrdI 1313 GACTTCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 267 CCGTGG C^C[GA][CT]GG
EaeI 200 TGGCCA [CT]^GGCC[GA]
EcoO65I 1811 GGTTACC G^GT[ACGT]ACC
EcoRI 317 GAATTC G^AATTC
HaeII 245 GGCGCC [GA]GCGC^[CT]
HhaI 246 GCGC GCG^C
Hin1I 245 GGCGCC G[GA]^CG[CT]C
HinP1I 246 GCGC G^CGC
KasI 245 GGCGCC G^GCGCC
MaeII 1059 ACGT A^CGT
MflI 1142 AGATCC [GA]^GATC[CT]
MluI 1194 ACGCGT A^CGCGT
MluNI 200 TGGCCA TGG^CCA
MscI 200 TGGCCA TGG^CCA
MslI 1096 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 245 GGCGCC GG^CGCC
NspI 2561 GCATGT [GA]CATG^[CT]
PstI 1700 CTGCAG CTGCA^G
SapI 1848 GCTCTTC GCTCTTC(1/4)
SexAI 1796 ACCTGGT A^CC[AT]GGT
SfoI 245 GGCGCC GGC^GCC
StuI 849 AGGCCT AGG^CCT
Tsp509I 318 AATT ^AATT
XbaI 1839 TCTAGA T^CTAGA
XhoII 1142 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 281 GGTACC G^GTACC
521 GGTACC
AccII 289 CGCG CG^CG
1195 CGCG
AocI 1266 CCTCAGG CC^T[ACGT]AGG
1343 CCTCAGG
Asp718I 281 GGTACC G^GTACC
521 GGTACC
BglI 354 GCCGCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
738 GCCGTACAGGC
BsaI 1625 GGTCTC GGTCTC(1/5)
2617 GGTCTC
BsgI 221 CTGCAC GTGCAG(16/14)
1817 CTGCAC
BspMI 576 ACCTGC ACCTGC(4/8)
1815 ACCTGC
BstUI 289 CGCG CG^CG
1195 CGCG
Bsu36I 1266 CCTCAGG CC^T[ACGT]AGG
1343 CCTCAGG
Eco81I 1266 CCTCAGG CC^T[ACGT]AGG
1343 CCTCAGG
HgaI 704 GACGC GACGC(5/10)
1283 GCGTC
KpnI 281 GGTACC GGTAC^C
521 GGTACC
MseI 666 TTAA T^TAA
2652 TTAA
MvnI 289 CGCG CG^CG
1195 CGCG
PvuII 806 CAGCTG CAG^CTG
1185 CAGCTG
SfcI 1700 CTGCAG C^T[GA][CT]AG
1973 CTGTAG
SmaI 504 CCCGGG CCC^GGG
893 CCCGGG
SmlI 683 CTCAAG C^T[CT][GA]AG
1437 CTCAAG
Tru9I 666 TTAA T^TAA
2652 TTAA
XmaCI 504 CCCGGG C^CCGGG
893 CCCGGG
XmaI 504 CCCGGG C^CCGGG
893 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SspBI StyI TfiI
TseI Tsp45I TspRI XcmI