##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspI AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BssHII BssSI Bst1107I
BstBI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MaeII
MamI MfeI MflI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 608 GTAGAC GT^[AC][GT]AC
AccII 755 CGCG CG^CG
AcsI 2323 GAATTT [GA]^AATT[CT]
AgeI 51 ACCGGT A^CCGGT
AlwI 1824 GATCC GGATC(4/5)
ApoI 2323 GAATTT [GA]^AATT[CT]
Asp700I 1091 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1787 GAGCAC G[AT]GC[AT]^C
AvaI 590 CCCGAG C^[CT]CG[GA]G
BalI 2345 TGGCCA TGG^CCA
BsiHKAI 1787 GAGCAC G[AT]GC[AT]^C
BsoBI 590 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1215 TGTACA T^GTACA
BsrBI 2032 GAGCGG CCGCTC(-3/-3)
BsrDI 296 CATTGC GCAATG(2/0)
BsrGI 1215 TGTACA T^GTACA
BstEII 1644 GGTAACC G^GT[ACGT]ACC
BstPI 1644 GGTAACC G^GT[ACGT]ACC
BstUI 755 CGCG CG^CG
BstXI 2416 CCACCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 1824 GATC ^GATC
DraIII 2201 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2345 TGGCCA [CT]^GGCC[GA]
EcoO65I 1644 GGTAACC G^GT[ACGT]ACC
MboI 1824 GATC ^GATC
MluNI 2345 TGGCCA TGG^CCA
MscI 2345 TGGCCA TGG^CCA
MseI 2100 TTAA T^TAA
MvnI 755 CGCG CG^CG
NaeI 1308 GCCGGC GCC^GGC
NcoI 238 CCATGG C^CATGG
NdeII 1824 GATC ^GATC
NgoMIV 1308 GCCGGC G^CCGGC
PinAI 51 ACCGGT A^CCGGT
Sau3AI 1824 GATC ^GATC
SbfI 1666 CCTGCAGG CCTGCA^GG
SmlI 122 CTTGAG C^T[CT][GA]AG
Sse8387I 1666 CCTGCAGG CCTGCA^GG
SspBI 1215 TGTACA T^GTACA
TaqI 1261 TCGA T^CGA
Tru9I 2100 TTAA T^TAA
TthHB8I 1261 TCGA T^CGA
XcmI 2283 CCATGCCAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1091 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 48 GGTACC G^GTACC
149 GGTACC
Asp718I 48 GGTACC G^GTACC
149 GGTACC
AviII 288 TGCGCA TGC^GCA
1053 TGCGCA
BanII 584 GAGCCC G[GA]GC[CT]^C
1145 GAGCCC
BbvCI 11 CCTCAGC CCTCAGC(-5/-2)
1766 CCTCAGC
BsaI 1263 GAGACC GGTCTC(1/5)
1612 GGTCTC
BsaWI 51 ACCGGT [AT]^CCGG[AT]
1072 ACCGGA
BsrFI 51 ACCGGT [GA]^CCGG[CT]
1308 GCCGGC
Cfr10I 51 ACCGGT [GA]^CCGG[CT]
1308 GCCGGC
DsaI 238 CCATGG C^C[GA][CT]GG
2405 CCGTGG
EcoNI 92 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
905 CCTCTAGGAGG
EcoT14I 238 CCATGG C^C[AT][AT]GG
1180 CCTTGG
FspI 288 TGCGCA TGC^GCA
1053 TGCGCA
KpnI 48 GGTACC GGTAC^C
149 GGTACC
PvuII 1506 CAGCTG CAG^CTG
1710 CAGCTG
SexAI 2202 ACCTGGT A^CC[AT]GGT
2221 ACCAGGT
SphI 2164 GCATGC GCATG^C
2378 GCATGC
StyI 238 CCATGG C^C[AT][AT]GG
1180 CCTTGG
TfiI 572 GAATC G^A[AT]TC
2470 GATTC
Tsp509I 376 AATT ^AATT
2324 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV NspI
PleI PstI RsaI Sau96I ScrFI
SfaNI SfcI StuI TseI Tsp45I
TspRI