##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BalI BbrPI BbvCI
BclI BglI BlnI BsaAI BsaBI
BsiWI BsmI BspDI BspLU11I BsrDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI Eco47III EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1814 GACGTC GACGT^C
AccI 1509 GTAGAC GT^[AC][GT]AC
AcsI 440 GAATTC [GA]^AATT[CT]
AflII 2496 CTTAAG C^TTAAG
AhdI 1697 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2625 GTGCAC G^TGCAC
ApaLI 2625 GTGCAC G^TGCAC
ApoI 440 GAATTC [GA]^AATT[CT]
AspEI 1697 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1013 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2689 GGATCC G^GATCC
BciVI 1914 GGATAC GTATCC(6/5)
BfrI 2496 CTTAAG C^TTAAG
BlpI 2166 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2166 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 327 TGTACA T^GTACA
BsrGI 327 TGTACA T^GTACA
BstAPI 917 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1051 GGTAACC G^GT[ACGT]ACC
BstPI 1051 GGTAACC G^GT[ACGT]ACC
BstXI 2127 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2166 GCTGAGC GC^T[ACGT]AGC
DraII 49 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 2368 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 975 CCATGG C^C[GA][CT]GG
EagI 832 CGGCCG C^GGCCG
Eam1105I 1697 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2210 CTCTTC CTCTTC(1/4)
EclXI 832 CGGCCG C^GGCCG
Eco52I 832 CGGCCG C^GGCCG
EcoNI 617 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 49 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1051 GGTAACC G^GT[ACGT]ACC
EcoRI 440 GAATTC G^AATTC
Ksp632I 2210 CTCTTC CTCTTC(1/4)
NcoI 975 CCATGG C^CATGG
NdeI 214 CATATG CA^TATG
PflFI 1013 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 49 GGGACCC [GA]G^G[AT]CC[CT]
ScaI 1095 AGTACT AGT^ACT
SspBI 327 TGTACA T^GTACA
SspI 2734 AATATT AAT^ATT
Tth111I 1013 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccII 97 CGCG CG^CG
494 CGCG
AccIII 141 TCCGGA T^CCGGA
1821 TCCGGA
AflIII 153 ACGTGT A^C[GA][CT]GT
1918 ACACGT
AlwNI 1423 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2161 CAGCAGCTG
AocI 1402 CCTCAGG CC^T[ACGT]AGG
1580 CCTGAGG
BbeI 29 GGCGCC GGCGC^C
252 GGCGCC
BcgI 482 CGATTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1466 GCAAGCCACTCG
BfaI 720 CTAG C^TAG
2653 CTAG
BglII 520 AGATCT A^GATCT
663 AGATCT
BseAI 141 TCCGGA T^CCGGA
1821 TCCGGA
BsiEI 832 CGGCCG CG[GA][CT]^CG
1062 CGACCG
BsmBI 1058 GAGACG CGTCTC(1/5)
1340 GAGACG
BspEI 141 TCCGGA T^CCGGA
1821 TCCGGA
BspHI 384 TCATGA T^CATGA
2383 TCATGA
BspMI 110 GCAGGT ACCTGC(4/8)
2089 GCAGGT
BsrBI 60 GAGCGG CCGCTC(-3/-3)
2685 GAGCGG
BsrFI 1362 ACCGGC [GA]^CCGG[CT]
2013 ACCGGC
BssSI 1148 CACGAG CACGAG(-5/-1)
2628 CACGAG
BstUI 97 CGCG CG^CG
494 CGCG
Bsu36I 1402 CCTCAGG CC^T[ACGT]AGG
1580 CCTGAGG
Cfr10I 1362 ACCGGC [GA]^CCGG[CT]
2013 ACCGGC
DraI 2253 TTTAAA TTT^AAA
2518 TTTAAA
Eco81I 1402 CCTCAGG CC^T[ACGT]AGG
1580 CCTGAGG
HgaI 1013 GACGC GACGC(5/10)
191 GCGTC
HindIII 1370 AAGCTT A^AGCTT
2592 AAGCTT
KasI 29 GGCGCC G^GCGCC
252 GGCGCC
MaeI 720 CTAG C^TAG
2653 CTAG
MroI 141 TCCGGA T^CCGGA
1821 TCCGGA
MvnI 97 CGCG CG^CG
494 CGCG
NarI 29 GGCGCC GG^CGCC
252 GGCGCC
PflMI 763 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2603 CCATCCTGTGG
PleI 730 GACTC GAGTC(4/5)
2051 GACTC
RcaI 384 TCATGA T^CATGA
2383 TCATGA
SfoI 29 GGCGCC GGC^GCC
252 GGCGCC
SmaI 45 CCCGGG CCC^GGG
1500 CCCGGG
SphI 1618 GCATGC GCATG^C
1731 GCATGC
TaqI 131 TCGA T^CGA
1170 TCGA
TfiI 559 GAATC G^A[AT]TC
716 GATTC
TthHB8I 131 TCGA T^CGA
1170 TCGA
Van91I 763 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2603 CCATCCTGTGG
XmaCI 45 CCCGGG C^CCGGG
1500 CCCGGG
XmaI 45 CCCGGG C^CCGGG
1500 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PshAI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII