################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## Acc65I AclI AgeI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AviII AvrII BalI BbrPI BbvCI BclI BglI BlnI BsaAI BsaBI BsiWI BsmI BspDI BspLU11I BsrDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI Eco47III EcoRV EcoT22I FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MluNI MscI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 1814 GACGTC GACGT^C AccI 1509 GTAGAC GT^[AC][GT]AC AcsI 440 GAATTC [GA]^AATT[CT] AflII 2496 CTTAAG C^TTAAG AhdI 1697 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2625 GTGCAC G^TGCAC ApaLI 2625 GTGCAC G^TGCAC ApoI 440 GAATTC [GA]^AATT[CT] AspEI 1697 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1013 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2689 GGATCC G^GATCC BciVI 1914 GGATAC GTATCC(6/5) BfrI 2496 CTTAAG C^TTAAG BlpI 2166 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2166 GCTGAGC GC^T[ACGT]AGC Bsp1407I 327 TGTACA T^GTACA BsrGI 327 TGTACA T^GTACA BstAPI 917 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1051 GGTAACC G^GT[ACGT]ACC BstPI 1051 GGTAACC G^GT[ACGT]ACC BstXI 2127 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2166 GCTGAGC GC^T[ACGT]AGC DraII 49 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 2368 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 975 CCATGG C^C[GA][CT]GG EagI 832 CGGCCG C^GGCCG Eam1105I 1697 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2210 CTCTTC CTCTTC(1/4) EclXI 832 CGGCCG C^GGCCG Eco52I 832 CGGCCG C^GGCCG EcoNI 617 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 49 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 1051 GGTAACC G^GT[ACGT]ACC EcoRI 440 GAATTC G^AATTC Ksp632I 2210 CTCTTC CTCTTC(1/4) NcoI 975 CCATGG C^CATGG NdeI 214 CATATG CA^TATG PflFI 1013 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 49 GGGACCC [GA]G^G[AT]CC[CT] ScaI 1095 AGTACT AGT^ACT SspBI 327 TGTACA T^GTACA SspI 2734 AATATT AAT^ATT Tth111I 1013 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### AccII 97 CGCG CG^CG 494 CGCG AccIII 141 TCCGGA T^CCGGA 1821 TCCGGA AflIII 153 ACGTGT A^C[GA][CT]GT 1918 ACACGT AlwNI 1423 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 2161 CAGCAGCTG AocI 1402 CCTCAGG CC^T[ACGT]AGG 1580 CCTGAGG BbeI 29 GGCGCC GGCGC^C 252 GGCGCC BcgI 482 CGATTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1466 GCAAGCCACTCG BfaI 720 CTAG C^TAG 2653 CTAG BglII 520 AGATCT A^GATCT 663 AGATCT BseAI 141 TCCGGA T^CCGGA 1821 TCCGGA BsiEI 832 CGGCCG CG[GA][CT]^CG 1062 CGACCG BsmBI 1058 GAGACG CGTCTC(1/5) 1340 GAGACG BspEI 141 TCCGGA T^CCGGA 1821 TCCGGA BspHI 384 TCATGA T^CATGA 2383 TCATGA BspMI 110 GCAGGT ACCTGC(4/8) 2089 GCAGGT BsrBI 60 GAGCGG CCGCTC(-3/-3) 2685 GAGCGG BsrFI 1362 ACCGGC [GA]^CCGG[CT] 2013 ACCGGC BssSI 1148 CACGAG CACGAG(-5/-1) 2628 CACGAG BstUI 97 CGCG CG^CG 494 CGCG Bsu36I 1402 CCTCAGG CC^T[ACGT]AGG 1580 CCTGAGG Cfr10I 1362 ACCGGC [GA]^CCGG[CT] 2013 ACCGGC DraI 2253 TTTAAA TTT^AAA 2518 TTTAAA Eco81I 1402 CCTCAGG CC^T[ACGT]AGG 1580 CCTGAGG HgaI 1013 GACGC GACGC(5/10) 191 GCGTC HindIII 1370 AAGCTT A^AGCTT 2592 AAGCTT KasI 29 GGCGCC G^GCGCC 252 GGCGCC MaeI 720 CTAG C^TAG 2653 CTAG MroI 141 TCCGGA T^CCGGA 1821 TCCGGA MvnI 97 CGCG CG^CG 494 CGCG NarI 29 GGCGCC GG^CGCC 252 GGCGCC PflMI 763 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2603 CCATCCTGTGG PleI 730 GACTC GAGTC(4/5) 2051 GACTC RcaI 384 TCATGA T^CATGA 2383 TCATGA SfoI 29 GGCGCC GGC^GCC 252 GGCGCC SmaI 45 CCCGGG CCC^GGG 1500 CCCGGG SphI 1618 GCATGC GCATG^C 1731 GCATGC TaqI 131 TCGA T^CGA 1170 TCGA TfiI 559 GAATC G^A[AT]TC 716 GATTC TthHB8I 131 TCGA T^CGA 1170 TCGA Van91I 763 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2603 CCATCCTGTGG XmaCI 45 CCCGGG C^CCGGG 1500 CCCGGG XmaI 45 CCCGGG C^CCGGG 1500 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PshAI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SmlI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII