##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AclI AflII Alw44I
ApaLI AscI AseI AsnI AspI
AviII AvrII BcgI BciVI BclI
BfrI BglII BlnI BsaBI BseAI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CpoI DraI DraIII EagI
EclXI Eco52I EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tru9I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 333 GGTACC G^GTACC
AccI 513 GTCTAC GT^[AC][GT]AC
AccII 899 CGCG CG^CG
AcsI 233 GAATTC [GA]^AATT[CT]
AgeI 362 ACCGGT A^CCGGT
AhdI 1523 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 936 AGCGCT AGC^GCT
ApoI 233 GAATTC [GA]^AATT[CT]
Asp718I 333 GGTACC G^GTACC
AspEI 1523 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1169 CACGTG CAC^GTG
BbvCI 85 CCTCAGC CCTCAGC(-5/-2)
BsaAI 1169 CACGTG [CT]AC^GT[GA]
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BsiEI 424 CGGTCG CG[GA][CT]^CG
BspDI 186 ATCGAT AT^CGAT
BstUI 899 CGCG CG^CG
BstXI 1783 CCAGGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 186 ATCGAT AT^CGAT
Eam1105I 1523 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 936 AGCGCT AGC^GCT
EcoRI 233 GAATTC G^AATTC
FseI 255 GGCCGGCC GGCCGG^CC
HgaI 897 GACGC GACGC(5/10)
KpnI 333 GGTACC GGTAC^C
MvnI 899 CGCG CG^CG
NaeI 256 GCCGGC GCC^GGC
NcoI 796 CCATGG C^CATGG
NgoMIV 256 GCCGGC G^CCGGC
PflMI 539 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 362 ACCGGT A^CCGGT
PmaCI 1169 CACGTG CAC^GTG
PmlI 1169 CACGTG CAC^GTG
PshAI 1781 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 857 CCTGCAGG CCTGCA^GG
SgrAI 361 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 857 CCTGCAGG CCTGCA^GG
Van91I 539 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1128 GGCGCC G[GA]^CG[CT]C
1472 GGCGCC
AlwNI 30 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
2020 CAGGAGCTG
AocI 498 CCTCAGG CC^T[ACGT]AGG
2211 CCTGAGG
Asp700I 600 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1603 GAAACTCTTC
BalI 1465 TGGCCA TGG^CCA
1653 TGGCCA
BbeI 1128 GGCGCC GGCGC^C
1472 GGCGCC
BlpI 315 GCTCAGC GC^T[ACGT]AGC
942 GCTGAGC
Bpu1102I 315 GCTCAGC GC^T[ACGT]AGC
942 GCTGAGC
BsaHI 1128 GGCGCC G[GA]^CG[CT]C
1472 GGCGCC
BsaI 450 GAGACC GGTCTC(1/5)
1457 GGTCTC
BsrFI 256 GCCGGC [GA]^CCGG[CT]
362 ACCGGT
Bsu36I 498 CCTCAGG CC^T[ACGT]AGG
2211 CCTGAGG
CelII 315 GCTCAGC GC^T[ACGT]AGC
942 GCTGAGC
Cfr10I 256 GCCGGC [GA]^CCGG[CT]
362 ACCGGT
DrdI 695 GACCACGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1315 GACCCCTCAGTC
EarI 1607 CTCTTC CTCTTC(1/4)
1762 CTCTTC
Eco81I 498 CCTCAGG CC^T[ACGT]AGG
2211 CCTGAGG
Hin1I 1128 GGCGCC G[GA]^CG[CT]C
1472 GGCGCC
KasI 1128 GGCGCC G^GCGCC
1472 GGCGCC
Ksp632I 1607 CTCTTC CTCTTC(1/4)
1762 CTCTTC
MluNI 1465 TGGCCA TGG^CCA
1653 TGGCCA
MscI 1465 TGGCCA TGG^CCA
1653 TGGCCA
NarI 1128 GGCGCC GG^CGCC
1472 GGCGCC
PaeR7I 174 CTCGAG C^TCGAG
1191 CTCGAG
PstI 27 CTGCAG CTGCA^G
858 CTGCAG
SacI 945 GAGCTC GAGCT^C
1588 GAGCTC
SfoI 1128 GGCGCC GGC^GCC
1472 GGCGCC
SmaI 145 CCCGGG CCC^GGG
484 CCCGGG
TfiI 159 GAATC G^A[AT]TC
347 GAATC
XhoI 174 CTCGAG C^TCGAG
1191 CTCGAG
XmaCI 145 CCCGGG C^CCGGG
484 CCCGGG
XmaI 145 CCCGGG C^CCGGG
484 CCCGGG
XmnI 600 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1603 GAAACTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
ApaI AspHI AvaI AvaII BamHI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstEII BstF5I
BstNI BstPI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII