##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AviII AvrII BamHI BcgI
BciVI BclI BfrI BlnI BsaBI
BseAI BsiEI BsiWI BspEI BspHI
BsrBI BssHII Bst1107I BstBI BstZ17I
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KspI MamI MluI MroI NdeI
NotI NsiI NspV PacI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 285 GGTACC G^GTACC
AccII 2183 CGCG CG^CG
AcyI 1292 GGCGCC G[GA]^CG[CT]C
AflIII 1052 ACATGT A^C[GA][CT]GT
AgeI 1338 ACCGGT A^CCGGT
Aor51HI 2352 AGCGCT AGC^GCT
Asp700I 1368 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 285 GGTACC G^GTACC
AspHI 1863 GAGCTC G[AT]GC[AT]^C
AspI 241 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1292 GGCGCC GGCGC^C
BbrPI 1120 CACGTG CAC^GTG
BbvCI 478 GCTGAGG CCTCAGC(-5/-2)
BsaAI 1120 CACGTG [CT]AC^GT[GA]
BsaHI 1292 GGCGCC G[GA]^CG[CT]C
BseRI 481 GAGGAG GAGGAG(10/8)
BsiHKAI 1863 GAGCTC G[AT]GC[AT]^C
BsmBI 496 GAGACG CGTCTC(1/5)
Bsp1407I 983 TGTACA T^GTACA
BspDI 2560 ATCGAT AT^CGAT
BspLU11I 1052 ACATGT A^CATGT
BsrGI 983 TGTACA T^GTACA
BssSI 181 CACGAG CACGAG(-5/-1)
BstEII 114 GGTGACC G^GT[ACGT]ACC
BstPI 114 GGTGACC G^GT[ACGT]ACC
BstUI 2183 CGCG CG^CG
ClaI 2560 ATCGAT AT^CGAT
DraIII 1699 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2352 AGCGCT AGC^GCT
EcoO65I 114 GGTGACC G^GT[ACGT]ACC
Hin1I 1292 GGCGCC G[GA]^CG[CT]C
HincII 2677 GTCAAC GT[CT]^[GA]AC
HindII 2677 GTCAAC GT[CT]^[GA]AC
KasI 1292 GGCGCC G^GCGCC
KpnI 285 GGTACC GGTAC^C
MfeI 2233 CAATTG C^AATTG
MunI 2233 CAATTG C^AATTG
MvnI 2183 CGCG CG^CG
NaeI 89 GCCGGC GCC^GGC
NarI 1292 GGCGCC GG^CGCC
NcoI 8 CCATGG C^CATGG
NgoMIV 89 GCCGGC G^CCGGC
NheI 2355 GCTAGC G^CTAGC
NruI 2182 TCGCGA TCG^CGA
NspI 1052 ACATGT [GA]CATG^[CT]
PaeR7I 2220 CTCGAG C^TCGAG
PflFI 241 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1005 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1338 ACCGGT A^CCGGT
PmaCI 1120 CACGTG CAC^GTG
PmlI 1120 CACGTG CAC^GTG
PshAI 1125 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1863 GAGCTC GAGCT^C
SapI 729 GCTCTTC GCTCTTC(1/4)
SfoI 1292 GGCGCC GGC^GCC
SmaI 917 CCCGGG CCC^GGG
SspBI 983 TGTACA T^GTACA
StuI 833 AGGCCT AGG^CCT
Tth111I 241 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1005 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2220 CTCGAG C^TCGAG
XmaCI 917 CCCGGG C^CCGGG
XmaI 917 CCCGGG C^CCGGG
XmnI 1368 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 373 GTAGAC GT^[AC][GT]AC
2714 GTCTAC
AcsI 1018 GAATTC [GA]^AATT[CT]
2501 GAATTC
AlwI 690 GATCC GGATC(4/5)
2563 GATCC
AocI 1992 CCTCAGG CC^T[ACGT]AGG
2203 CCTCAGG
ApoI 1018 GAATTC [GA]^AATT[CT]
2501 GAATTC
BalI 230 TGGCCA TGG^CCA
2437 TGGCCA
BbsI 136 GAAGAC GAAGAC(2/6)
2695 GTCTTC
BglI 631 GCCGTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2057 GCCTGGAAGGC
BglII 308 AGATCT A^GATCT
602 AGATCT
BlpI 53 GCTCAGC GC^T[ACGT]AGC
1173 GCTCAGC
Bpu1102I 53 GCTCAGC GC^T[ACGT]AGC
1173 GCTCAGC
BpuAI 136 GAAGAC GAAGAC(2/6)
2695 GTCTTC
BsaI 1447 GAGACC GGTCTC(1/5)
2394 GGTCTC
BsaWI 692 TCCGGT [AT]^CCGG[AT]
1338 ACCGGT
BsgI 925 CTGCAC GTGCAG(16/14)
1732 CTGCAC
BsmI 431 GCATTC GAATGC(1/-1)
932 GCATTC
BspMI 197 ACCTGC ACCTGC(4/8)
799 ACCTGC
BstYI 308 AGATCT [GA]^GATC[CT]
602 AGATCT
Bsu36I 1992 CCTCAGG CC^T[ACGT]AGG
2203 CCTCAGG
CelII 53 GCTCAGC GC^T[ACGT]AGC
1173 GCTCAGC
EarI 730 CTCTTC CTCTTC(1/4)
2541 GAAGAG
Eco81I 1992 CCTCAGG CC^T[ACGT]AGG
2203 CCTCAGG
EcoRI 1018 GAATTC G^AATTC
2501 GAATTC
Ksp632I 730 CTCTTC CTCTTC(1/4)
2541 GAAGAG
MflI 308 AGATCT [GA]^GATC[CT]
602 AGATCT
MluNI 230 TGGCCA TGG^CCA
2437 TGGCCA
MscI 230 TGGCCA TGG^CCA
2437 TGGCCA
SmlI 322 CTCAAG C^T[CT][GA]AG
2220 CTCGAG
XhoII 308 AGATCT [GA]^GATC[CT]
602 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI