################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccIII AclI AflII AhdI Alw44I ApaLI AscI AseI AsnI AspEI AviII AvrII BamHI BcgI BciVI BclI BfrI BlnI BsaBI BseAI BsiEI BsiWI BspEI BspHI BsrBI BssHII Bst1107I BstBI BstZ17I CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI HgaI HindIII HpaI KspI MamI MluI MroI NdeI NotI NsiI NspV PacI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### Acc65I 285 GGTACC G^GTACC AccII 2183 CGCG CG^CG AcyI 1292 GGCGCC G[GA]^CG[CT]C AflIII 1052 ACATGT A^C[GA][CT]GT AgeI 1338 ACCGGT A^CCGGT Aor51HI 2352 AGCGCT AGC^GCT Asp700I 1368 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 285 GGTACC G^GTACC AspHI 1863 GAGCTC G[AT]GC[AT]^C AspI 241 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1292 GGCGCC GGCGC^C BbrPI 1120 CACGTG CAC^GTG BbvCI 478 GCTGAGG CCTCAGC(-5/-2) BsaAI 1120 CACGTG [CT]AC^GT[GA] BsaHI 1292 GGCGCC G[GA]^CG[CT]C BseRI 481 GAGGAG GAGGAG(10/8) BsiHKAI 1863 GAGCTC G[AT]GC[AT]^C BsmBI 496 GAGACG CGTCTC(1/5) Bsp1407I 983 TGTACA T^GTACA BspDI 2560 ATCGAT AT^CGAT BspLU11I 1052 ACATGT A^CATGT BsrGI 983 TGTACA T^GTACA BssSI 181 CACGAG CACGAG(-5/-1) BstEII 114 GGTGACC G^GT[ACGT]ACC BstPI 114 GGTGACC G^GT[ACGT]ACC BstUI 2183 CGCG CG^CG ClaI 2560 ATCGAT AT^CGAT DraIII 1699 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2352 AGCGCT AGC^GCT EcoO65I 114 GGTGACC G^GT[ACGT]ACC Hin1I 1292 GGCGCC G[GA]^CG[CT]C HincII 2677 GTCAAC GT[CT]^[GA]AC HindII 2677 GTCAAC GT[CT]^[GA]AC KasI 1292 GGCGCC G^GCGCC KpnI 285 GGTACC GGTAC^C MfeI 2233 CAATTG C^AATTG MunI 2233 CAATTG C^AATTG MvnI 2183 CGCG CG^CG NaeI 89 GCCGGC GCC^GGC NarI 1292 GGCGCC GG^CGCC NcoI 8 CCATGG C^CATGG NgoMIV 89 GCCGGC G^CCGGC NheI 2355 GCTAGC G^CTAGC NruI 2182 TCGCGA TCG^CGA NspI 1052 ACATGT [GA]CATG^[CT] PaeR7I 2220 CTCGAG C^TCGAG PflFI 241 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1005 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1338 ACCGGT A^CCGGT PmaCI 1120 CACGTG CAC^GTG PmlI 1120 CACGTG CAC^GTG PshAI 1125 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1863 GAGCTC GAGCT^C SapI 729 GCTCTTC GCTCTTC(1/4) SfoI 1292 GGCGCC GGC^GCC SmaI 917 CCCGGG CCC^GGG SspBI 983 TGTACA T^GTACA StuI 833 AGGCCT AGG^CCT Tth111I 241 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1005 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2220 CTCGAG C^TCGAG XmaCI 917 CCCGGG C^CCGGG XmaI 917 CCCGGG C^CCGGG XmnI 1368 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 373 GTAGAC GT^[AC][GT]AC 2714 GTCTAC AcsI 1018 GAATTC [GA]^AATT[CT] 2501 GAATTC AlwI 690 GATCC GGATC(4/5) 2563 GATCC AocI 1992 CCTCAGG CC^T[ACGT]AGG 2203 CCTCAGG ApoI 1018 GAATTC [GA]^AATT[CT] 2501 GAATTC BalI 230 TGGCCA TGG^CCA 2437 TGGCCA BbsI 136 GAAGAC GAAGAC(2/6) 2695 GTCTTC BglI 631 GCCGTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2057 GCCTGGAAGGC BglII 308 AGATCT A^GATCT 602 AGATCT BlpI 53 GCTCAGC GC^T[ACGT]AGC 1173 GCTCAGC Bpu1102I 53 GCTCAGC GC^T[ACGT]AGC 1173 GCTCAGC BpuAI 136 GAAGAC GAAGAC(2/6) 2695 GTCTTC BsaI 1447 GAGACC GGTCTC(1/5) 2394 GGTCTC BsaWI 692 TCCGGT [AT]^CCGG[AT] 1338 ACCGGT BsgI 925 CTGCAC GTGCAG(16/14) 1732 CTGCAC BsmI 431 GCATTC GAATGC(1/-1) 932 GCATTC BspMI 197 ACCTGC ACCTGC(4/8) 799 ACCTGC BstYI 308 AGATCT [GA]^GATC[CT] 602 AGATCT Bsu36I 1992 CCTCAGG CC^T[ACGT]AGG 2203 CCTCAGG CelII 53 GCTCAGC GC^T[ACGT]AGC 1173 GCTCAGC EarI 730 CTCTTC CTCTTC(1/4) 2541 GAAGAG Eco81I 1992 CCTCAGG CC^T[ACGT]AGG 2203 CCTCAGG EcoRI 1018 GAATTC G^AATTC 2501 GAATTC Ksp632I 730 CTCTTC CTCTTC(1/4) 2541 GAAGAG MflI 308 AGATCT [GA]^GATC[CT] 602 AGATCT MluNI 230 TGGCCA TGG^CCA 2437 TGGCCA MscI 230 TGGCCA TGG^CCA 2437 TGGCCA SmlI 322 CTCAAG C^T[CT][GA]AG 2220 CTCGAG XhoII 308 AGATCT [GA]^GATC[CT] 602 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI