##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AclI AcyI AflII AflIII
AhdI Aor51HI ApaI AscI AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsiWI BsmI BspDI BspLU11I
BssHII BssSI Bst1107I BstZ17I CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI Hin1I HpaI KasI
KspI MaeII MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI Psp1406I RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 1735 GGTACC G^GTACC
AccI 284 GTAGAC GT^[AC][GT]AC
AccII 54 CGCG CG^CG
AgeI 1921 ACCGGT A^CCGGT
Alw44I 41 GTGCAC G^TGCAC
AlwNI 2097 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 41 GTGCAC G^TGCAC
AseI 3322 ATTAAT AT^TAAT
AsnI 3322 ATTAAT AT^TAAT
Asp700I 676 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1735 GGTACC G^GTACC
AvaI 1459 CCCGGG C^[CT]CG[GA]G
BalI 2093 TGGCCA TGG^CCA
BanII 1943 GGGCTC G[GA]GC[CT]^C
BcgI 1765 GCAGCTGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 954 TGATCA T^GATCA
BfaI 2357 CTAG C^TAG
BsaBI 1499 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1998 GTGCAG GTGCAG(16/14)
BsiEI 1087 CGATCG CG[GA][CT]^CG
BsmBI 63 GAGACG CGTCTC(1/5)
BsoBI 1459 CCCGGG C^[CT]CG[GA]G
BsrBI 11 CCGCTC CCGCTC(-3/-3)
BstEII 2347 GGTCACC G^GT[ACGT]ACC
BstPI 2347 GGTCACC G^GT[ACGT]ACC
BstUI 54 CGCG CG^CG
BstXI 1104 CCATTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 2093 TGGCCA [CT]^GGCC[GA]
EcoO65I 2347 GGTCACC G^GT[ACGT]ACC
EcoT22I 2383 ATGCAT ATGCA^T
FauI 3245 CCCGC CCCGC(4/6)
FbaI 954 TGATCA T^GATCA
HaeII 1669 AGCGCC [GA]GCGC^[CT]
HgaI 1064 GACGC GACGC(5/10)
HincII 3223 GTTGAC GT[CT]^[GA]AC
HindII 3223 GTTGAC GT[CT]^[GA]AC
HindIII 1703 AAGCTT A^AGCTT
KpnI 1735 GGTACC GGTAC^C
MaeI 2357 CTAG C^TAG
MamI 1499 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2093 TGGCCA TGG^CCA
MscI 2093 TGGCCA TGG^CCA
MvnI 54 CGCG CG^CG
NdeI 860 CATATG CA^TATG
NsiI 2383 ATGCAT ATGCA^T
NspI 237 GCATGC [GA]CATG^[CT]
PinAI 1921 ACCGGT A^CCGGT
PshBI 3322 ATTAAT AT^TAAT
PstI 20 CTGCAG CTGCA^G
PvuI 1087 CGATCG CGAT^CG
SapI 527 GAAGAGC GCTCTTC(1/4)
SmaI 1459 CCCGGG CCC^GGG
SmlI 2082 CTCAAG C^T[CT][GA]AG
SphI 237 GCATGC GCATG^C
StuI 459 AGGCCT AGG^CCT
XmaCI 1459 CCCGGG C^CCGGG
XmaI 1459 CCCGGG C^CCGGG
XmnI 676 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 59 TCCGGA T^CCGGA
1120 TCCGGA
BanI 1015 GGCACC G^G[CT][GA]CC
1735 GGTACC
BbsI 230 GAAGAC GAAGAC(2/6)
147 GTCTTC
BpuAI 230 GAAGAC GAAGAC(2/6)
147 GTCTTC
BseAI 59 TCCGGA T^CCGGA
1120 TCCGGA
BseRI 2659 GAGGAG GAGGAG(10/8)
2771 GAGGAG
BspEI 59 TCCGGA T^CCGGA
1120 TCCGGA
BspMI 919 ACCTGC ACCTGC(4/8)
351 GCAGGT
BsrFI 1921 ACCGGT [GA]^CCGG[CT]
2684 GCCGGT
BstAPI 43 GCACTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2072 GCATATCCTGC
BstBI 1324 TTCGAA TT^CGAA
2463 TTCGAA
BstYI 492 GGATCT [GA]^GATC[CT]
2300 GGATCT
CfoI 55 GCGC GCG^C
1670 GCGC
Cfr10I 1921 ACCGGT [GA]^CCGG[CT]
2684 GCCGGT
DraI 828 TTTAAA TTT^AAA
2941 TTTAAA
DraII 1989 AGGACCT [GA]G^G[ACGT]CC[CT]
2910 AGGTCCC
EcoNI 2078 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2950 CCTAAGAAAGG
EcoO109I 1989 AGGACCT [GA]G^G[ACGT]CC[CT]
2910 AGGTCCC
HhaI 55 GCGC GCG^C
1670 GCGC
HinP1I 55 GCGC G^CGC
1670 GCGC
MflI 492 GGATCT [GA]^GATC[CT]
2300 GGATCT
MroI 59 TCCGGA T^CCGGA
1120 TCCGGA
NcoI 1591 CCATGG C^CATGG
2394 CCATGG
NspV 1324 TTCGAA TT^CGAA
2463 TTCGAA
PflMI 1591 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2291 CCAGCCTTTGG
PpuMI 1989 AGGACCT [GA]G^G[AT]CC[CT]
2910 AGGTCCC
SfuI 1324 TTCGAA TT^CGAA
2463 TTCGAA
Van91I 1591 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2291 CCAGCCTTTGG
XhoII 492 GGATCT [GA]^GATC[CT]
2300 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaII BbvI
BcnI BmyI BpmI BsaI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspHI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
Bsu36I Cac8I Cfr13I DdeI DpnII
DsaI EarI Eco81I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I