##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
Aor51HI ApaI AscI Asp700I Asp718I
AspI AviII AvrII BbeI BbrPI
BbvCI BclI BcnI BlnI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 1548 CGCG CG^CG
AcsI 1891 GAATTC [GA]^AATT[CT]
AflII 842 CTTAAG C^TTAAG
AflIII 1619 ACACGT A^C[GA][CT]GT
Alw44I 1551 GTGCAC G^TGCAC
ApaLI 1551 GTGCAC G^TGCAC
ApoI 1891 GAATTC [GA]^AATT[CT]
AseI 1451 ATTAAT AT^TAAT
AsnI 1451 ATTAAT AT^TAAT
BamHI 1825 GGATCC G^GATCC
BanI 1006 GGCACC G^G[CT][GA]CC
BcgI 1811 CGAGAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 842 CTTAAG C^TTAAG
BglI 938 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1186 AGATCT A^GATCT
BlpI 2631 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2631 GCTGAGC GC^T[ACGT]AGC
BsmI 2587 GCATTC GAATGC(1/-1)
BsrBI 1273 GAGCGG CCGCTC(-3/-3)
BsrFI 915 GCCGGT [GA]^CCGG[CT]
BssSI 1870 CTCGTG CACGAG(-5/-1)
BstUI 1548 CGCG CG^CG
CelII 2631 GCTGAGC GC^T[ACGT]AGC
Cfr10I 915 GCCGGT [GA]^CCGG[CT]
DrdI 2506 GACTCCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 135 CCGTGG C^C[GA][CT]GG
EcoNI 95 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1891 GAATTC G^AATTC
HincII 319 GTTGAC GT[CT]^[GA]AC
HindII 319 GTTGAC GT[CT]^[GA]AC
MvnI 1548 CGCG CG^CG
PaeR7I 1130 CTCGAG C^TCGAG
PshBI 1451 ATTAAT AT^TAAT
PstI 984 CTGCAG CTGCA^G
SacI 1984 GAGCTC GAGCT^C
StuI 1709 AGGCCT AGG^CCT
XhoI 1130 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 878 GACGTC GACGT^C
2147 GACGTC
AcyI 878 GACGTC G[GA]^CG[CT]C
2147 GACGTC
AhdI 1685 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2239 GACCAGGTGTC
AspEI 1685 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2239 GACCAGGTGTC
BsaAI 284 CACGTA [CT]AC^GT[GA]
2002 TACGTG
BsaHI 878 GACGTC G[GA]^CG[CT]C
2147 GACGTC
BseRI 2447 CTCCTC GAGGAG(10/8)
1303 GAGGAG
BsmBI 1338 CGTCTC CGTCTC(1/5)
1732 CGTCTC
BspMI 749 ACCTGC ACCTGC(4/8)
2546 ACCTGC
DraIII 284 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
1917 CACAGTGTG
Eam1105I 1685 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2239 GACCAGGTGTC
HapII 916 CCGG C^CGG
2249 CCGG
Hin1I 878 GACGTC G[GA]^CG[CT]C
2147 GACGTC
HpaII 916 CCGG C^CGG
2249 CCGG
MspI 916 CCGG C^CGG
2249 CCGG
PflMI 283 CCACGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
485 CCAAATGATGG
PvuII 977 CAGCTG CAG^CTG
987 CAGCTG
SexAI 1239 ACCAGGT A^CC[AT]GGT
2240 ACCAGGT
SfcI 957 CTGTAG C^T[GA][CT]AG
984 CTGCAG
SphI 753 GCATGC GCATG^C
837 GCATGC
TfiI 414 GAATC G^A[AT]TC
2622 GATTC
Van91I 283 CCACGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
485 CCAAATGATGG
XcmI 375 CCAGAGATGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1932 CCACAGTGCGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BalI
BanII BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII