################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## Acc65I AccI AccIII AclI AgeI Aor51HI ApaI AscI Asp700I Asp718I AspI AviII AvrII BbeI BbrPI BbvCI BclI BcnI BlnI BsaBI BsaI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 1548 CGCG CG^CG AcsI 1891 GAATTC [GA]^AATT[CT] AflII 842 CTTAAG C^TTAAG AflIII 1619 ACACGT A^C[GA][CT]GT Alw44I 1551 GTGCAC G^TGCAC ApaLI 1551 GTGCAC G^TGCAC ApoI 1891 GAATTC [GA]^AATT[CT] AseI 1451 ATTAAT AT^TAAT AsnI 1451 ATTAAT AT^TAAT BamHI 1825 GGATCC G^GATCC BanI 1006 GGCACC G^G[CT][GA]CC BcgI 1811 CGAGAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 842 CTTAAG C^TTAAG BglI 938 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1186 AGATCT A^GATCT BlpI 2631 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2631 GCTGAGC GC^T[ACGT]AGC BsmI 2587 GCATTC GAATGC(1/-1) BsrBI 1273 GAGCGG CCGCTC(-3/-3) BsrFI 915 GCCGGT [GA]^CCGG[CT] BssSI 1870 CTCGTG CACGAG(-5/-1) BstUI 1548 CGCG CG^CG CelII 2631 GCTGAGC GC^T[ACGT]AGC Cfr10I 915 GCCGGT [GA]^CCGG[CT] DrdI 2506 GACTCCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 135 CCGTGG C^C[GA][CT]GG EcoNI 95 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1891 GAATTC G^AATTC HincII 319 GTTGAC GT[CT]^[GA]AC HindII 319 GTTGAC GT[CT]^[GA]AC MvnI 1548 CGCG CG^CG PaeR7I 1130 CTCGAG C^TCGAG PshBI 1451 ATTAAT AT^TAAT PstI 984 CTGCAG CTGCA^G SacI 1984 GAGCTC GAGCT^C StuI 1709 AGGCCT AGG^CCT XhoI 1130 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 878 GACGTC GACGT^C 2147 GACGTC AcyI 878 GACGTC G[GA]^CG[CT]C 2147 GACGTC AhdI 1685 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2239 GACCAGGTGTC AspEI 1685 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2239 GACCAGGTGTC BsaAI 284 CACGTA [CT]AC^GT[GA] 2002 TACGTG BsaHI 878 GACGTC G[GA]^CG[CT]C 2147 GACGTC BseRI 2447 CTCCTC GAGGAG(10/8) 1303 GAGGAG BsmBI 1338 CGTCTC CGTCTC(1/5) 1732 CGTCTC BspMI 749 ACCTGC ACCTGC(4/8) 2546 ACCTGC DraIII 284 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG 1917 CACAGTGTG Eam1105I 1685 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2239 GACCAGGTGTC HapII 916 CCGG C^CGG 2249 CCGG Hin1I 878 GACGTC G[GA]^CG[CT]C 2147 GACGTC HpaII 916 CCGG C^CGG 2249 CCGG MspI 916 CCGG C^CGG 2249 CCGG PflMI 283 CCACGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 485 CCAAATGATGG PvuII 977 CAGCTG CAG^CTG 987 CAGCTG SexAI 1239 ACCAGGT A^CC[AT]GGT 2240 ACCAGGT SfcI 957 CTGTAG C^T[GA][CT]AG 984 CTGCAG SphI 753 GCATGC GCATG^C 837 GCATGC TfiI 414 GAATC G^A[AT]TC 2622 GATTC Van91I 283 CCACGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 485 CCAAATGATGG XcmI 375 CCAGAGATGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1932 CCACAGTGCGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanII BbsI BbvI BciVI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII