##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AccIII AclI AgeI Alw44I AocI
ApaLI AscI Asp700I AviII AvrII
BalI BamHI BbrPI BciVI BclI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
BspEI BspHI BspLU11I BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII CpoI DraI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MamI MfeI MluNI
MroI MscI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I RcaI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1665 GACGTC GACGT^C
Acc65I 2046 GGTACC G^GTACC
AccI 702 GTCTAC GT^[AC][GT]AC
AflII 2069 CTTAAG C^TTAAG
AflIII 1489 ACGCGT A^C[GA][CT]GT
AhdI 1068 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 16 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 2083 ATTAAT AT^TAAT
AsnI 2083 ATTAAT AT^TAAT
Asp718I 2046 GGTACC G^GTACC
AspEI 1068 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 862 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 221 GGCGCC GGCGC^C
BbvCI 300 GCTGAGG CCTCAGC(-5/-2)
BcgI 239 CGAGCCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2069 CTTAAG C^TTAAG
BsaI 1627 GAGACC GGTCTC(1/5)
BsmFI 1540 GGGAC GGGAC(10/14)
Bsp1407I 556 TGTACA T^GTACA
BspDI 1287 ATCGAT AT^CGAT
BspMI 2043 GCAGGT ACCTGC(4/8)
BsrBI 733 CCGCTC CCGCTC(-3/-3)
BsrDI 1523 CATTGC GCAATG(2/0)
BsrGI 556 TGTACA T^GTACA
BstXI 1283 CCAGATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1287 ATCGAT AT^CGAT
DraIII 1254 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1509 GACTTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 45 CGGCCG C^GGCCG
Eam1105I 1068 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 45 CGGCCG C^GGCCG
Eco52I 45 CGGCCG C^GGCCG
EcoNI 1999 CCTTCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 221 GGCGCC G^GCGCC
KpnI 2046 GGTACC GGTAC^C
MluI 1489 ACGCGT A^CGCGT
NarI 221 GGCGCC GG^CGCC
NcoI 790 CCATGG C^CATGG
PflFI 862 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2083 ATTAAT AT^TAAT
PvuI 2145 CGATCG CGAT^CG
PvuII 874 CAGCTG CAG^CTG
SexAI 2259 ACCTGGT A^CC[AT]GGT
SfoI 221 GGCGCC GGC^GCC
SmaI 292 CCCGGG CCC^GGG
SspBI 556 TGTACA T^GTACA
TfiI 1469 GATTC G^A[AT]TC
Tth111I 862 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1171 CCATCTCTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 292 CCCGGG C^CCGGG
XmaI 292 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Aor51HI 110 AGCGCT AGC^GCT
1332 AGCGCT
ApaI 215 GGGCCC GGGCC^C
305 GGGCCC
BbsI 921 GAAGAC GAAGAC(2/6)
1662 GAAGAC
BglI 60 GCCGCGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1899 GCCAAGATGGC
BpuAI 921 GAAGAC GAAGAC(2/6)
1662 GAAGAC
BsiEI 45 CGGCCG CG[GA][CT]^CG
2145 CGATCG
BsrFI 242 GCCGGC [GA]^CCGG[CT]
390 GCCGGC
BstYI 88 AGATCC [GA]^GATC[CT]
1733 AGATCC
Cfr10I 242 GCCGGC [GA]^CCGG[CT]
390 GCCGGC
DsaI 30 CCACGG C^C[GA][CT]GG
790 CCATGG
EaeI 45 CGGCCG [CT]^GGCC[GA]
1678 CGGCCA
Eco47III 110 AGCGCT AGC^GCT
1332 AGCGCT
HgaI 1167 GACGC GACGC(5/10)
1123 GCGTC
MflI 88 AGATCC [GA]^GATC[CT]
1733 AGATCC
MslI 351 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
713 CATGGAAATG
MspA1I 318 CAGCGG C[AC]G^C[GT]G
874 CAGCTG
NaeI 242 GCCGGC GCC^GGC
390 GCCGGC
NgoMIV 242 GCCGGC G^CCGGC
390 GCCGGC
PpuMI 1540 GGGACCC [GA]G^G[AT]CC[CT]
2064 AGGACCT
PstI 1177 CTGCAG CTGCA^G
1729 CTGCAG
SacI 1080 GAGCTC GAGCT^C
1087 GAGCTC
SapI 2267 GAAGAGC GCTCTTC(1/4)
1607 GCTCTTC
StuI 9 AGGCCT AGG^CCT
271 AGGCCT
XhoII 88 AGATCC [GA]^GATC[CT]
1733 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsoBI Bsp1286I BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I