##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AviII AvrII BamHI BbeI
BcgI BglI BlnI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT14I FauI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KspI MluI MroI
NaeI NarI NcoI NgoMIV NotI
NruI PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI XbaI XcmI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 925 GGTACC G^GTACC
AccI 1316 GTCTAC GT^[AC][GT]AC
AciI 2853 GCGG CCGC(-3/-1)
AflII 1724 CTTAAG C^TTAAG
Asp718I 925 GGTACC G^GTACC
AvaI 2094 CTCGAG C^[CT]CG[GA]G
BanI 925 GGTACC G^G[CT][GA]CC
BbrPI 1823 CACGTG CAC^GTG
BciVI 1683 GTATCC GTATCC(6/5)
BcnI 1902 CCCGG CC^[GC]GG
BfrI 1724 CTTAAG C^TTAAG
BlpI 553 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 553 GCTCAGC GC^T[ACGT]AGC
BsaAI 1823 CACGTG [CT]AC^GT[GA]
BsaBI 998 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3326 GAGACC GGTCTC(1/5)
BsgI 2523 GTGCAG GTGCAG(16/14)
BsoBI 2094 CTCGAG C^[CT]CG[GA]G
BspHI 2253 TCATGA T^CATGA
BstBI 2295 TTCGAA TT^CGAA
CelII 553 GCTCAGC GC^T[ACGT]AGC
DsaI 921 CCACGG C^C[GA][CT]GG
EcoNI 1650 CCTGTTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 412 AAGCTT A^AGCTT
KpnI 925 GGTACC GGTAC^C
MamI 998 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 1902 CCCGG CC^[GC]GG
NdeI 2375 CATATG CA^TATG
NspV 2295 TTCGAA TT^CGAA
PaeR7I 2094 CTCGAG C^TCGAG
PmaCI 1823 CACGTG CAC^GTG
PmlI 1823 CACGTG CAC^GTG
PstI 1531 CTGCAG CTGCA^G
RcaI 2253 TCATGA T^CATGA
SfuI 2295 TTCGAA TT^CGAA
XhoI 2094 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspI 932 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2104 GACTATGTC
BbsI 3421 GAAGAC GAAGAC(2/6)
2004 GTCTTC
BbvCI 830 CCTCAGC CCTCAGC(-5/-2)
2786 CCTCAGC
BclI 802 TGATCA T^GATCA
3058 TGATCA
BpmI 121 CTCCAG CTGGAG(16/14)
309 CTCCAG
BpuAI 3421 GAAGAC GAAGAC(2/6)
2004 GTCTTC
BsmI 704 GAATGC GAATGC(1/-1)
2943 GCATTC
BspLU11I 252 ACATGT A^CATGT
298 ACATGT
BssHII 358 GCGCGC G^CGCGC
360 GCGCGC
BssSI 2360 CTCGTG CACGAG(-5/-1)
3002 CTCGTG
DraI 66 TTTAAA TTT^AAA
151 TTTAAA
EcoRI 2218 GAATTC G^AATTC
2533 GAATTC
EcoT22I 211 ATGCAT ATGCA^T
2378 ATGCAT
FbaI 802 TGATCA T^GATCA
3058 TGATCA
HphI 2824 TCACC GGTGA(8/7)
2947 TCACC
MfeI 813 CAATTG C^AATTG
2903 CAATTG
MunI 813 CAATTG C^AATTG
2903 CAATTG
NsiI 211 ATGCAT ATGCA^T
2378 ATGCAT
PflFI 932 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2104 GACTATGTC
PflMI 771 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2543 CCAGACTCTGG
PleI 2546 GACTC GAGTC(4/5)
2250 GAGTC
PvuII 2641 CAGCTG CAG^CTG
2789 CAGCTG
SacI 2097 GAGCTC GAGCT^C
2909 GAGCTC
ScaI 2281 AGTACT AGT^ACT
2394 AGTACT
SfaNI 2722 GATGC GCATC(5/9)
788 GCATC
Tsp45I 1743 GTGAC ^GT[GC]AC
2968 GTCAC
Tth111I 932 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2104 GACTATGTC
Van91I 771 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2543 CCAGACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanII BbvI BfaI BglII
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NheI NlaIII
NlaIV NspI PpuMI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII