################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BamHI BbeI BcgI BglI BlnI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT14I FauI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KspI MluI MroI NaeI NarI NcoI NgoMIV NotI NruI PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 925 GGTACC G^GTACC AccI 1316 GTCTAC GT^[AC][GT]AC AciI 2853 GCGG CCGC(-3/-1) AflII 1724 CTTAAG C^TTAAG Asp718I 925 GGTACC G^GTACC AvaI 2094 CTCGAG C^[CT]CG[GA]G BanI 925 GGTACC G^G[CT][GA]CC BbrPI 1823 CACGTG CAC^GTG BciVI 1683 GTATCC GTATCC(6/5) BcnI 1902 CCCGG CC^[GC]GG BfrI 1724 CTTAAG C^TTAAG BlpI 553 GCTCAGC GC^T[ACGT]AGC Bpu1102I 553 GCTCAGC GC^T[ACGT]AGC BsaAI 1823 CACGTG [CT]AC^GT[GA] BsaBI 998 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3326 GAGACC GGTCTC(1/5) BsgI 2523 GTGCAG GTGCAG(16/14) BsoBI 2094 CTCGAG C^[CT]CG[GA]G BspHI 2253 TCATGA T^CATGA BstBI 2295 TTCGAA TT^CGAA CelII 553 GCTCAGC GC^T[ACGT]AGC DsaI 921 CCACGG C^C[GA][CT]GG EcoNI 1650 CCTGTTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HindIII 412 AAGCTT A^AGCTT KpnI 925 GGTACC GGTAC^C MamI 998 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NciI 1902 CCCGG CC^[GC]GG NdeI 2375 CATATG CA^TATG NspV 2295 TTCGAA TT^CGAA PaeR7I 2094 CTCGAG C^TCGAG PmaCI 1823 CACGTG CAC^GTG PmlI 1823 CACGTG CAC^GTG PstI 1531 CTGCAG CTGCA^G RcaI 2253 TCATGA T^CATGA SfuI 2295 TTCGAA TT^CGAA XhoI 2094 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AspI 932 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2104 GACTATGTC BbsI 3421 GAAGAC GAAGAC(2/6) 2004 GTCTTC BbvCI 830 CCTCAGC CCTCAGC(-5/-2) 2786 CCTCAGC BclI 802 TGATCA T^GATCA 3058 TGATCA BpmI 121 CTCCAG CTGGAG(16/14) 309 CTCCAG BpuAI 3421 GAAGAC GAAGAC(2/6) 2004 GTCTTC BsmI 704 GAATGC GAATGC(1/-1) 2943 GCATTC BspLU11I 252 ACATGT A^CATGT 298 ACATGT BssHII 358 GCGCGC G^CGCGC 360 GCGCGC BssSI 2360 CTCGTG CACGAG(-5/-1) 3002 CTCGTG DraI 66 TTTAAA TTT^AAA 151 TTTAAA EcoRI 2218 GAATTC G^AATTC 2533 GAATTC EcoT22I 211 ATGCAT ATGCA^T 2378 ATGCAT FbaI 802 TGATCA T^GATCA 3058 TGATCA HphI 2824 TCACC GGTGA(8/7) 2947 TCACC MfeI 813 CAATTG C^AATTG 2903 CAATTG MunI 813 CAATTG C^AATTG 2903 CAATTG NsiI 211 ATGCAT ATGCA^T 2378 ATGCAT PflFI 932 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2104 GACTATGTC PflMI 771 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2543 CCAGACTCTGG PleI 2546 GACTC GAGTC(4/5) 2250 GAGTC PvuII 2641 CAGCTG CAG^CTG 2789 CAGCTG SacI 2097 GAGCTC GAGCT^C 2909 GAGCTC ScaI 2281 AGTACT AGT^ACT 2394 AGTACT SfaNI 2722 GATGC GCATC(5/9) 788 GCATC Tsp45I 1743 GTGAC ^GT[GC]AC 2968 GTCAC Tth111I 932 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2104 GACTATGTC Van91I 771 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2543 CCAGACTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BalI BanII BbvI BfaI BglII BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NheI NlaIII NlaIV NspI PpuMI RsaI Sau3AI Sau96I ScrFI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII