################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI AhdI ApaI AscI AseI AsnI Asp700I AspEI AvaI AvrII BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KspI MamI MluI MroI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI Sse8387I SspBI SspI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1111 GGTACC G^GTACC AccI 1728 GTAGAC GT^[AC][GT]AC AcsI 1615 AAATTT [GA]^AATT[CT] AcyI 1320 GACGCC G[GA]^CG[CT]C Alw44I 1987 GTGCAC G^TGCAC Aor51HI 110 AGCGCT AGC^GCT ApaLI 1987 GTGCAC G^TGCAC ApoI 1615 AAATTT [GA]^AATT[CT] Asp718I 1111 GGTACC G^GTACC AviII 1927 TGCGCA TGC^GCA BalI 526 TGGCCA TGG^CCA BclI 411 TGATCA T^GATCA BsaAI 1381 TACGTA [CT]AC^GT[GA] BsaHI 1320 GACGCC G[GA]^CG[CT]C BsaWI 327 ACCGGA [AT]^CCGG[AT] BsmBI 364 GAGACG CGTCTC(1/5) BspHI 437 TCATGA T^CATGA BsrDI 2402 CATTGC GCAATG(2/0) DraII 1358 AGGACCC [GA]G^G[ACGT]CC[CT] DrdI 1360 GACCCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 526 TGGCCA [CT]^GGCC[GA] Eco47III 110 AGCGCT AGC^GCT EcoO109I 1358 AGGACCC [GA]G^G[ACGT]CC[CT] FbaI 411 TGATCA T^GATCA FspI 1927 TGCGCA TGC^GCA HaeII 110 AGCGCT [GA]GCGC^[CT] HapII 328 CCGG C^CGG Hin1I 1320 GACGCC G[GA]^CG[CT]C HpaII 328 CCGG C^CGG KpnI 1111 GGTACC GGTAC^C MluNI 526 TGGCCA TGG^CCA MscI 526 TGGCCA TGG^CCA MspI 328 CCGG C^CGG PpuMI 1358 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1800 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 437 TCATGA T^CATGA SacI 1300 GAGCTC GAGCT^C ScaI 47 AGTACT AGT^ACT SnaBI 1381 TACGTA TAC^GTA StuI 449 AGGCCT AGG^CCT XbaI 484 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AspHI 1300 GAGCTC G[AT]GC[AT]^C 1987 GTGCAC AspI 366 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2485 GACTCTGTC BbvCI 19 GCTGAGG CCTCAGC(-5/-2) 2461 GCTGAGG BglI 764 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1309 GCCTGTGTGGC BpmI 1776 CTCCAG CTGGAG(16/14) 67 CTGGAG BsaI 2505 GAGACC GGTCTC(1/5) 2443 GGTCTC BsgI 1563 CTGCAC GTGCAG(16/14) 313 GTGCAG BsiHKAI 1300 GAGCTC G[AT]GC[AT]^C 1987 GTGCAC BsmI 537 GAATGC GAATGC(1/-1) 1098 GCATTC BssSI 205 CACGAG CACGAG(-5/-1) 1975 CTCGTG BstEII 1491 GGTAACC G^GT[ACGT]ACC 1837 GGTCACC BstPI 1491 GGTAACC G^GT[ACGT]ACC 1837 GGTCACC BstYI 1155 AGATCC [GA]^GATC[CT] 1873 AGATCC CfoI 111 GCGC GCG^C 1928 GCGC DraIII 1569 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG 2435 CACAGTGTG EcoO65I 1491 GGTAACC G^GT[ACGT]ACC 1837 GGTCACC FauI 2383 CCCGC CCCGC(4/6) 768 GCGGG HgaI 667 GACGC GACGC(5/10) 1320 GACGC HhaI 111 GCGC GCG^C 1928 GCGC HinP1I 111 GCGC G^CGC 1928 GCGC HincII 1163 GTCAAC GT[CT]^[GA]AC 2100 GTTGAC HindII 1163 GTCAAC GT[CT]^[GA]AC 2100 GTTGAC MfeI 1244 CAATTG C^AATTG 1632 CAATTG MflI 1155 AGATCC [GA]^GATC[CT] 1873 AGATCC MunI 1244 CAATTG C^AATTG 1632 CAATTG NcoI 116 CCATGG C^CATGG 1943 CCATGG PflFI 366 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2485 GACTCTGTC PflMI 2434 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2510 CCAAAGAGTGG SapI 76 GAAGAGC GCTCTTC(1/4) 1608 GAAGAGC SmlI 1072 CTCAAG C^T[CT][GA]AG 1830 CTCAAG SphI 388 GCATGC GCATG^C 1198 GCATGC TaqI 92 TCGA T^CGA 278 TCGA Tth111I 366 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2485 GACTCTGTC TthHB8I 92 TCGA T^CGA 278 TCGA Van91I 2434 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2510 CCAAAGAGTGG XhoII 1155 AGATCC [GA]^GATC[CT] 1873 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI AocI AvaII BanI BanII BbsI BbvI BfaI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I Cfr13I DdeI DpnII DsaI EarI Eco81I EcoNI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI