##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI ApaI AscI AseI
AsnI Asp700I AspEI AvaI AvrII
BamHI BbeI BbrPI BcgI BciVI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KasI KspI
MamI MluI MroI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
SspBI SspI SwaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1111 GGTACC G^GTACC
AccI 1728 GTAGAC GT^[AC][GT]AC
AcsI 1615 AAATTT [GA]^AATT[CT]
AcyI 1320 GACGCC G[GA]^CG[CT]C
Alw44I 1987 GTGCAC G^TGCAC
Aor51HI 110 AGCGCT AGC^GCT
ApaLI 1987 GTGCAC G^TGCAC
ApoI 1615 AAATTT [GA]^AATT[CT]
Asp718I 1111 GGTACC G^GTACC
AviII 1927 TGCGCA TGC^GCA
BalI 526 TGGCCA TGG^CCA
BclI 411 TGATCA T^GATCA
BsaAI 1381 TACGTA [CT]AC^GT[GA]
BsaHI 1320 GACGCC G[GA]^CG[CT]C
BsaWI 327 ACCGGA [AT]^CCGG[AT]
BsmBI 364 GAGACG CGTCTC(1/5)
BspHI 437 TCATGA T^CATGA
BsrDI 2402 CATTGC GCAATG(2/0)
DraII 1358 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 1360 GACCCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 526 TGGCCA [CT]^GGCC[GA]
Eco47III 110 AGCGCT AGC^GCT
EcoO109I 1358 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 411 TGATCA T^GATCA
FspI 1927 TGCGCA TGC^GCA
HaeII 110 AGCGCT [GA]GCGC^[CT]
HapII 328 CCGG C^CGG
Hin1I 1320 GACGCC G[GA]^CG[CT]C
HpaII 328 CCGG C^CGG
KpnI 1111 GGTACC GGTAC^C
MluNI 526 TGGCCA TGG^CCA
MscI 526 TGGCCA TGG^CCA
MspI 328 CCGG C^CGG
PpuMI 1358 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1800 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 437 TCATGA T^CATGA
SacI 1300 GAGCTC GAGCT^C
ScaI 47 AGTACT AGT^ACT
SnaBI 1381 TACGTA TAC^GTA
StuI 449 AGGCCT AGG^CCT
XbaI 484 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AspHI 1300 GAGCTC G[AT]GC[AT]^C
1987 GTGCAC
AspI 366 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2485 GACTCTGTC
BbvCI 19 GCTGAGG CCTCAGC(-5/-2)
2461 GCTGAGG
BglI 764 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1309 GCCTGTGTGGC
BpmI 1776 CTCCAG CTGGAG(16/14)
67 CTGGAG
BsaI 2505 GAGACC GGTCTC(1/5)
2443 GGTCTC
BsgI 1563 CTGCAC GTGCAG(16/14)
313 GTGCAG
BsiHKAI 1300 GAGCTC G[AT]GC[AT]^C
1987 GTGCAC
BsmI 537 GAATGC GAATGC(1/-1)
1098 GCATTC
BssSI 205 CACGAG CACGAG(-5/-1)
1975 CTCGTG
BstEII 1491 GGTAACC G^GT[ACGT]ACC
1837 GGTCACC
BstPI 1491 GGTAACC G^GT[ACGT]ACC
1837 GGTCACC
BstYI 1155 AGATCC [GA]^GATC[CT]
1873 AGATCC
CfoI 111 GCGC GCG^C
1928 GCGC
DraIII 1569 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2435 CACAGTGTG
EcoO65I 1491 GGTAACC G^GT[ACGT]ACC
1837 GGTCACC
FauI 2383 CCCGC CCCGC(4/6)
768 GCGGG
HgaI 667 GACGC GACGC(5/10)
1320 GACGC
HhaI 111 GCGC GCG^C
1928 GCGC
HinP1I 111 GCGC G^CGC
1928 GCGC
HincII 1163 GTCAAC GT[CT]^[GA]AC
2100 GTTGAC
HindII 1163 GTCAAC GT[CT]^[GA]AC
2100 GTTGAC
MfeI 1244 CAATTG C^AATTG
1632 CAATTG
MflI 1155 AGATCC [GA]^GATC[CT]
1873 AGATCC
MunI 1244 CAATTG C^AATTG
1632 CAATTG
NcoI 116 CCATGG C^CATGG
1943 CCATGG
PflFI 366 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2485 GACTCTGTC
PflMI 2434 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2510 CCAAAGAGTGG
SapI 76 GAAGAGC GCTCTTC(1/4)
1608 GAAGAGC
SmlI 1072 CTCAAG C^T[CT][GA]AG
1830 CTCAAG
SphI 388 GCATGC GCATG^C
1198 GCATGC
TaqI 92 TCGA T^CGA
278 TCGA
Tth111I 366 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2485 GACTCTGTC
TthHB8I 92 TCGA T^CGA
278 TCGA
Van91I 2434 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2510 CCAAAGAGTGG
XhoII 1155 AGATCC [GA]^GATC[CT]
1873 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI AvaII BanI BanII
BbsI BbvI BfaI BmyI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstXI Bsu36I
Cac8I Cfr13I DdeI DpnII DsaI
EarI Eco81I EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI