################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BglI BlnI BsaBI BsiEI BsiWI BsmI BsoBI BspDI BspHI BspLU11I BsrFI BssHII Bst1107I BstXI BstZ17I Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FauI FseI FspI HaeII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 235 GTCTAC GT^[AC][GT]AC AccII 1440 CGCG CG^CG AccIII 144 TCCGGA T^CCGGA AcyI 989 GACGCC G[GA]^CG[CT]C AflII 1565 CTTAAG C^TTAAG AflIII 239 ACGTGT A^C[GA][CT]GT AlwI 467 GATCC GGATC(4/5) AocI 620 CCTAAGG CC^T[ACGT]AGG Asp700I 322 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 1965 GGGCTC G[GA]GC[CT]^C BbsI 1974 GTCTTC GAAGAC(2/6) BcgI 1207 CGATAGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 739 TGATCA T^GATCA BcnI 1308 CCCGG CC^[GC]GG BfrI 1565 CTTAAG C^TTAAG BglII 1693 AGATCT A^GATCT BpuAI 1974 GTCTTC GAAGAC(2/6) BsaAI 238 TACGTG [CT]AC^GT[GA] BsaHI 989 GACGCC G[GA]^CG[CT]C BsaI 2015 GGTCTC GGTCTC(1/5) BsaWI 144 TCCGGA [AT]^CCGG[AT] BseAI 144 TCCGGA T^CCGGA BsmBI 1181 GAGACG CGTCTC(1/5) Bsp1407I 2081 TGTACA T^GTACA BspEI 144 TCCGGA T^CCGGA BsrBI 1368 GAGCGG CCGCTC(-3/-3) BsrGI 2081 TGTACA T^GTACA BstBI 1571 TTCGAA TT^CGAA BstUI 1440 CGCG CG^CG BstYI 1693 AGATCT [GA]^GATC[CT] Bsu36I 620 CCTAAGG CC^T[ACGT]AGG CfoI 1441 GCGC GCG^C DraIII 1645 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2011 CCGTGG C^C[GA][CT]GG Eco81I 620 CCTAAGG CC^T[ACGT]AGG EcoNI 1523 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 432 ATGCAT ATGCA^T FbaI 739 TGATCA T^GATCA HhaI 1441 GCGC GCG^C Hin1I 989 GACGCC G[GA]^CG[CT]C HinP1I 1441 GCGC G^CGC MflI 1693 AGATCT [GA]^GATC[CT] MroI 144 TCCGGA T^CCGGA MslI 1502 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 800 CAGCTG C[AC]G^C[GT]G MvnI 1440 CGCG CG^CG NciI 1308 CCCGG CC^[GC]GG NsiI 432 ATGCAT ATGCA^T NspI 430 GCATGC [GA]CATG^[CT] NspV 1571 TTCGAA TT^CGAA PshAI 989 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1004 CTGCAG CTGCA^G PvuII 800 CAGCTG CAG^CTG SfuI 1571 TTCGAA TT^CGAA SphI 430 GCATGC GCATG^C SspBI 2081 TGTACA T^GTACA XhoII 1693 AGATCT [GA]^GATC[CT] XmnI 322 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Alw44I 166 GTGCAC G^TGCAC 754 GTGCAC AlwNI 764 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 800 CAGCTGCTG ApaLI 166 GTGCAC G^TGCAC 754 GTGCAC AvaII 1144 GGTCC G^G[AT]CC 1401 GGTCC BanI 282 GGTGCC G^G[CT][GA]CC 492 GGTGCC BlpI 631 GCTGAGC GC^T[ACGT]AGC 1580 GCTCAGC BpmI 218 CTGGAG CTGGAG(16/14) 1365 CTGGAG Bpu1102I 631 GCTGAGC GC^T[ACGT]AGC 1580 GCTCAGC BseRI 1148 CTCCTC GAGGAG(10/8) 1472 GAGGAG BsrDI 1282 CATTGC GCAATG(2/0) 1944 CATTGC BsrI 162 ACTGG ACTGG(1/-1) 1610 CCAGT BssSI 745 CACGAG CACGAG(-5/-1) 500 CTCGTG BstAPI 1034 GCATGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1298 GCAGACTATGC BstF5I 154 GGATG GGATG(2/0) 253 GGATG CelII 631 GCTGAGC GC^T[ACGT]AGC 1580 GCTCAGC DraII 886 AGGCCCT [GA]G^G[ACGT]CC[CT] 1134 AGGCCCT EcoO109I 886 AGGCCCT [GA]G^G[ACGT]CC[CT] 1134 AGGCCCT FokI 154 GGATG GGATG(9/13) 253 GGATG HapII 145 CCGG C^CGG 1309 CCGG HindIII 855 AAGCTT A^AGCTT 1993 AAGCTT HpaII 145 CCGG C^CGG 1309 CCGG MspI 145 CCGG C^CGG 1309 CCGG SapI 1318 GAAGAGC GCTCTTC(1/4) 1412 GAAGAGC SfcI 713 CTACAG C^T[GA][CT]AG 1004 CTGCAG TaqI 1206 TCGA T^CGA 1572 TCGA TthHB8I 1206 TCGA T^CGA 1572 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI BbvI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BstEII BstNI BstPI Cac8I Cfr13I DdeI DpnII EarI EcoO65I EcoRII EcoT14I Fnu4HI HaeIII HgaI HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI