##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BciVI BglI BlnI BsaBI
BsiEI BsiWI BsmI BsoBI BspDI
BspHI BspLU11I BsrFI BssHII Bst1107I
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FauI FseI FspI HaeII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 235 GTCTAC GT^[AC][GT]AC
AccII 1440 CGCG CG^CG
AccIII 144 TCCGGA T^CCGGA
AcyI 989 GACGCC G[GA]^CG[CT]C
AflII 1565 CTTAAG C^TTAAG
AflIII 239 ACGTGT A^C[GA][CT]GT
AlwI 467 GATCC GGATC(4/5)
AocI 620 CCTAAGG CC^T[ACGT]AGG
Asp700I 322 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 1965 GGGCTC G[GA]GC[CT]^C
BbsI 1974 GTCTTC GAAGAC(2/6)
BcgI 1207 CGATAGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 739 TGATCA T^GATCA
BcnI 1308 CCCGG CC^[GC]GG
BfrI 1565 CTTAAG C^TTAAG
BglII 1693 AGATCT A^GATCT
BpuAI 1974 GTCTTC GAAGAC(2/6)
BsaAI 238 TACGTG [CT]AC^GT[GA]
BsaHI 989 GACGCC G[GA]^CG[CT]C
BsaI 2015 GGTCTC GGTCTC(1/5)
BsaWI 144 TCCGGA [AT]^CCGG[AT]
BseAI 144 TCCGGA T^CCGGA
BsmBI 1181 GAGACG CGTCTC(1/5)
Bsp1407I 2081 TGTACA T^GTACA
BspEI 144 TCCGGA T^CCGGA
BsrBI 1368 GAGCGG CCGCTC(-3/-3)
BsrGI 2081 TGTACA T^GTACA
BstBI 1571 TTCGAA TT^CGAA
BstUI 1440 CGCG CG^CG
BstYI 1693 AGATCT [GA]^GATC[CT]
Bsu36I 620 CCTAAGG CC^T[ACGT]AGG
CfoI 1441 GCGC GCG^C
DraIII 1645 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2011 CCGTGG C^C[GA][CT]GG
Eco81I 620 CCTAAGG CC^T[ACGT]AGG
EcoNI 1523 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 432 ATGCAT ATGCA^T
FbaI 739 TGATCA T^GATCA
HhaI 1441 GCGC GCG^C
Hin1I 989 GACGCC G[GA]^CG[CT]C
HinP1I 1441 GCGC G^CGC
MflI 1693 AGATCT [GA]^GATC[CT]
MroI 144 TCCGGA T^CCGGA
MslI 1502 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 800 CAGCTG C[AC]G^C[GT]G
MvnI 1440 CGCG CG^CG
NciI 1308 CCCGG CC^[GC]GG
NsiI 432 ATGCAT ATGCA^T
NspI 430 GCATGC [GA]CATG^[CT]
NspV 1571 TTCGAA TT^CGAA
PshAI 989 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1004 CTGCAG CTGCA^G
PvuII 800 CAGCTG CAG^CTG
SfuI 1571 TTCGAA TT^CGAA
SphI 430 GCATGC GCATG^C
SspBI 2081 TGTACA T^GTACA
XhoII 1693 AGATCT [GA]^GATC[CT]
XmnI 322 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 166 GTGCAC G^TGCAC
754 GTGCAC
AlwNI 764 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
800 CAGCTGCTG
ApaLI 166 GTGCAC G^TGCAC
754 GTGCAC
AvaII 1144 GGTCC G^G[AT]CC
1401 GGTCC
BanI 282 GGTGCC G^G[CT][GA]CC
492 GGTGCC
BlpI 631 GCTGAGC GC^T[ACGT]AGC
1580 GCTCAGC
BpmI 218 CTGGAG CTGGAG(16/14)
1365 CTGGAG
Bpu1102I 631 GCTGAGC GC^T[ACGT]AGC
1580 GCTCAGC
BseRI 1148 CTCCTC GAGGAG(10/8)
1472 GAGGAG
BsrDI 1282 CATTGC GCAATG(2/0)
1944 CATTGC
BsrI 162 ACTGG ACTGG(1/-1)
1610 CCAGT
BssSI 745 CACGAG CACGAG(-5/-1)
500 CTCGTG
BstAPI 1034 GCATGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1298 GCAGACTATGC
BstF5I 154 GGATG GGATG(2/0)
253 GGATG
CelII 631 GCTGAGC GC^T[ACGT]AGC
1580 GCTCAGC
DraII 886 AGGCCCT [GA]G^G[ACGT]CC[CT]
1134 AGGCCCT
EcoO109I 886 AGGCCCT [GA]G^G[ACGT]CC[CT]
1134 AGGCCCT
FokI 154 GGATG GGATG(9/13)
253 GGATG
HapII 145 CCGG C^CGG
1309 CCGG
HindIII 855 AAGCTT A^AGCTT
1993 AAGCTT
HpaII 145 CCGG C^CGG
1309 CCGG
MspI 145 CCGG C^CGG
1309 CCGG
SapI 1318 GAAGAGC GCTCTTC(1/4)
1412 GAAGAGC
SfcI 713 CTACAG C^T[GA][CT]AG
1004 CTGCAG
TaqI 1206 TCGA T^CGA
1572 TCGA
TthHB8I 1206 TCGA T^CGA
1572 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BbvI BfaI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BssKI BstEII
BstNI BstPI Cac8I Cfr13I DdeI
DpnII EarI EcoO65I EcoRII EcoT14I
Fnu4HI HaeIII HgaI HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI