##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
ApaI ApoI AscI AspEI AspI
AviII AvrII BalI BbsI BfrI
BlnI BlpI Bpu1102I BpuAI BsiEI
BsiWI BsmI BspLU11I BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII Cfr10I CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HindIII HpaI Ksp632I
KspI MfeI MluI MluNI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SphI Sse8387I SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 1862 GACGTC GACGT^C
Acc65I 1499 GGTACC G^GTACC
AccI 840 GTCTAC GT^[AC][GT]AC
AccIII 1058 TCCGGA T^CCGGA
AflIII 736 ACGTGT A^C[GA][CT]GT
AlwNI 1314 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 780 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1381 AGCGCT AGC^GCT
AseI 1706 ATTAAT AT^TAAT
AsnI 1706 ATTAAT AT^TAAT
Asp718I 1499 GGTACC G^GTACC
BbeI 1693 GGCGCC GGCGC^C
BbrPI 2324 CACGTG CAC^GTG
BbvCI 1427 CCTCAGC CCTCAGC(-5/-2)
BcgI 529 GCACAGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 33 GGATAC GTATCC(6/5)
BclI 80 TGATCA T^GATCA
BglI 1558 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2324 CACGTG [CT]AC^GT[GA]
BsaBI 2489 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1058 TCCGGA [AT]^CCGG[AT]
BseAI 1058 TCCGGA T^CCGGA
Bsp1407I 739 TGTACA T^GTACA
BspEI 1058 TCCGGA T^CCGGA
BspHI 602 TCATGA T^CATGA
BspMI 1242 ACCTGC ACCTGC(4/8)
BsrDI 1252 GCAATG GCAATG(2/0)
BsrGI 739 TGTACA T^GTACA
Bsu36I 780 CCTGAGG CC^T[ACGT]AGG
DraIII 474 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 856 CCACGG C^C[GA][CT]GG
EaeI 1168 TGGCCG [CT]^GGCC[GA]
Eco47III 1381 AGCGCT AGC^GCT
Eco81I 780 CCTGAGG CC^T[ACGT]AGG
EcoRV 2139 GATATC GAT^ATC
FbaI 80 TGATCA T^GATCA
HincII 630 GTTGAC GT[CT]^[GA]AC
HindII 630 GTTGAC GT[CT]^[GA]AC
KasI 1693 GGCGCC G^GCGCC
KpnI 1499 GGTACC GGTAC^C
MamI 2489 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1058 TCCGGA T^CCGGA
NarI 1693 GGCGCC GG^CGCC
NspI 61 ACATGC [GA]CATG^[CT]
PaeR7I 183 CTCGAG C^TCGAG
PmaCI 2324 CACGTG CAC^GTG
PmlI 2324 CACGTG CAC^GTG
PshAI 2522 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1706 ATTAAT AT^TAAT
PvuII 645 CAGCTG CAG^CTG
RcaI 602 TCATGA T^CATGA
ScaI 412 AGTACT AGT^ACT
SfoI 1693 GGCGCC GGC^GCC
SmaI 253 CCCGGG CCC^GGG
SmlI 183 CTCGAG C^T[CT][GA]AG
SpeI 2566 ACTAGT A^CTAGT
SspBI 739 TGTACA T^GTACA
StuI 583 AGGCCT AGG^CCT
XhoI 183 CTCGAG C^TCGAG
XmaCI 253 CCCGGG C^CCGGG
XmaI 253 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Alw44I 315 GTGCAC G^TGCAC
1844 GTGCAC
ApaLI 315 GTGCAC G^TGCAC
1844 GTGCAC
Asp700I 1262 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1340 GAAAAACTTC
AvaI 183 CTCGAG C^[CT]CG[GA]G
253 CCCGGG
BamHI 1156 GGATCC G^GATCC
1993 GGATCC
BsoBI 183 CTCGAG C^[CT]CG[GA]G
253 CCCGGG
BspDI 804 ATCGAT AT^CGAT
2145 ATCGAT
BsrBI 1171 CCGCTC CCGCTC(-3/-3)
2404 CCGCTC
BstAPI 207 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
770 GCAAGAAGTGC
ClaI 804 ATCGAT AT^CGAT
2145 ATCGAT
MspA1I 645 CAGCTG C[AC]G^C[GT]G
1911 CAGCGG
PstI 520 CTGCAG CTGCA^G
2515 CTGCAG
SacI 186 GAGCTC GAGCT^C
753 GAGCTC
XcmI 616 CCAATGTCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1108 CCACATCCCACATGG
XmnI 1262 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1340 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanI BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII