################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII BamHI BciVI BclI BfrI BglII BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I ClaI CpoI DpnII DraI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MboI MfeI MflI MluI MroI MseI MunI MvnI NaeI NdeI NdeII NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AocI 1358 CCTCAGG CC^T[ACGT]AGG Aor51HI 538 AGCGCT AGC^GCT AspHI 89 GAGCTC G[AT]GC[AT]^C AvrII 1436 CCTAGG C^CTAGG BbeI 883 GGCGCC GGCGC^C BbrPI 1613 CACGTG CAC^GTG BcgI 408 GCAGTGAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 1436 CCTAGG C^CTAGG BlpI 1192 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1192 GCTCAGC GC^T[ACGT]AGC BsaAI 1613 CACGTG [CT]AC^GT[GA] BsiHKAI 89 GAGCTC G[AT]GC[AT]^C BsmI 820 GAATGC GAATGC(1/-1) BspMI 942 GCAGGT ACCTGC(4/8) BsrDI 837 CATTGC GCAATG(2/0) BsrFI 725 ACCGGC [GA]^CCGG[CT] Bsu36I 1358 CCTCAGG CC^T[ACGT]AGG CelII 1192 GCTCAGC GC^T[ACGT]AGC Cfr10I 725 ACCGGC [GA]^CCGG[CT] DrdI 1594 GACGCAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 547 CCATGG C^C[GA][CT]GG Eco47III 538 AGCGCT AGC^GCT Eco81I 1358 CCTCAGG CC^T[ACGT]AGG EcoNI 772 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 560 GTCAAC GT[CT]^[GA]AC HindII 560 GTCAAC GT[CT]^[GA]AC KasI 883 GGCGCC G^GCGCC MaeII 1614 ACGT A^CGT MslI 1238 CACACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 883 GGCGCC GG^CGCC NcoI 547 CCATGG C^CATGG NheI 1414 GCTAGC G^CTAGC PmaCI 1613 CACGTG CAC^GTG PmlI 1613 CACGTG CAC^GTG SacI 89 GAGCTC GAGCT^C SfiI 719 GGCCGTACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 883 GGCGCC GGC^GCC SmaI 1281 CCCGGG CCC^GGG SphI 766 GCATGC GCATG^C StuI 432 AGGCCT AGG^CCT TaqI 417 TCGA T^CGA TfiI 1495 GAATC G^A[AT]TC TthHB8I 417 TCGA T^CGA XmaCI 1281 CCCGGG C^CCGGG XmaI 1281 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 883 GGCGCC G[GA]^CG[CT]C 1600 GGCGTC AlwNI 258 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1526 CAGCATCTG ApaI 690 GGGCCC GGGCC^C 1363 GGGCCC AspI 365 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 827 GACATTGTC AvaI 1281 CCCGGG C^[CT]CG[GA]G 1641 CCCGAG BalI 1451 TGGCCA TGG^CCA 1621 TGGCCA BbvCI 860 GCTGAGG CCTCAGC(-5/-2) 878 GCTGAGG BsaHI 883 GGCGCC G[GA]^CG[CT]C 1600 GGCGTC BsgI 5 GTGCAG GTGCAG(16/14) 602 GTGCAG BsoBI 1281 CCCGGG C^[CT]CG[GA]G 1641 CCCGAG BsrI 529 ACTGG ACTGG(1/-1) 564 ACTGG BstXI 1130 CCAGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1612 CCACGTGGCTGG DraIII 1340 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 1731 CACCCTGTG HaeII 538 AGCGCT [GA]GCGC^[CT] 883 GGCGCC HgaI 1594 GACGC GACGC(5/10) 1601 GCGTC Hin1I 883 GGCGCC G[GA]^CG[CT]C 1600 GGCGTC MluNI 1451 TGGCCA TGG^CCA 1621 TGGCCA MscI 1451 TGGCCA TGG^CCA 1621 TGGCCA PflFI 365 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 827 GACATTGTC PflMI 1400 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1525 CCAGCATCTGG PshAI 296 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1134 GACCCTGGTC PstI 534 CTGCAG CTGCA^G 932 CTGCAG SmlI 111 CTTGAG C^T[CT][GA]AG 1649 CTCAAG Tth111I 365 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 827 GACATTGTC Van91I 1400 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1525 CCAGCATCTGG XcmI 952 CCAGGAGTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1432 CCAGCCTAGGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI