##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BamHI BciVI BclI
BfrI BglII BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
ClaI CpoI DpnII DraI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MboI MfeI MflI MluI MroI
MseI MunI MvnI NaeI NdeI
NdeII NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I XbaI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AocI 1358 CCTCAGG CC^T[ACGT]AGG
Aor51HI 538 AGCGCT AGC^GCT
AspHI 89 GAGCTC G[AT]GC[AT]^C
AvrII 1436 CCTAGG C^CTAGG
BbeI 883 GGCGCC GGCGC^C
BbrPI 1613 CACGTG CAC^GTG
BcgI 408 GCAGTGAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1436 CCTAGG C^CTAGG
BlpI 1192 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1192 GCTCAGC GC^T[ACGT]AGC
BsaAI 1613 CACGTG [CT]AC^GT[GA]
BsiHKAI 89 GAGCTC G[AT]GC[AT]^C
BsmI 820 GAATGC GAATGC(1/-1)
BspMI 942 GCAGGT ACCTGC(4/8)
BsrDI 837 CATTGC GCAATG(2/0)
BsrFI 725 ACCGGC [GA]^CCGG[CT]
Bsu36I 1358 CCTCAGG CC^T[ACGT]AGG
CelII 1192 GCTCAGC GC^T[ACGT]AGC
Cfr10I 725 ACCGGC [GA]^CCGG[CT]
DrdI 1594 GACGCAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 547 CCATGG C^C[GA][CT]GG
Eco47III 538 AGCGCT AGC^GCT
Eco81I 1358 CCTCAGG CC^T[ACGT]AGG
EcoNI 772 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 560 GTCAAC GT[CT]^[GA]AC
HindII 560 GTCAAC GT[CT]^[GA]AC
KasI 883 GGCGCC G^GCGCC
MaeII 1614 ACGT A^CGT
MslI 1238 CACACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 883 GGCGCC GG^CGCC
NcoI 547 CCATGG C^CATGG
NheI 1414 GCTAGC G^CTAGC
PmaCI 1613 CACGTG CAC^GTG
PmlI 1613 CACGTG CAC^GTG
SacI 89 GAGCTC GAGCT^C
SfiI 719 GGCCGTACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 883 GGCGCC GGC^GCC
SmaI 1281 CCCGGG CCC^GGG
SphI 766 GCATGC GCATG^C
StuI 432 AGGCCT AGG^CCT
TaqI 417 TCGA T^CGA
TfiI 1495 GAATC G^A[AT]TC
TthHB8I 417 TCGA T^CGA
XmaCI 1281 CCCGGG C^CCGGG
XmaI 1281 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 883 GGCGCC G[GA]^CG[CT]C
1600 GGCGTC
AlwNI 258 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1526 CAGCATCTG
ApaI 690 GGGCCC GGGCC^C
1363 GGGCCC
AspI 365 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
827 GACATTGTC
AvaI 1281 CCCGGG C^[CT]CG[GA]G
1641 CCCGAG
BalI 1451 TGGCCA TGG^CCA
1621 TGGCCA
BbvCI 860 GCTGAGG CCTCAGC(-5/-2)
878 GCTGAGG
BsaHI 883 GGCGCC G[GA]^CG[CT]C
1600 GGCGTC
BsgI 5 GTGCAG GTGCAG(16/14)
602 GTGCAG
BsoBI 1281 CCCGGG C^[CT]CG[GA]G
1641 CCCGAG
BsrI 529 ACTGG ACTGG(1/-1)
564 ACTGG
BstXI 1130 CCAGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1612 CCACGTGGCTGG
DraIII 1340 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1731 CACCCTGTG
HaeII 538 AGCGCT [GA]GCGC^[CT]
883 GGCGCC
HgaI 1594 GACGC GACGC(5/10)
1601 GCGTC
Hin1I 883 GGCGCC G[GA]^CG[CT]C
1600 GGCGTC
MluNI 1451 TGGCCA TGG^CCA
1621 TGGCCA
MscI 1451 TGGCCA TGG^CCA
1621 TGGCCA
PflFI 365 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
827 GACATTGTC
PflMI 1400 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1525 CCAGCATCTGG
PshAI 296 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1134 GACCCTGGTC
PstI 534 CTGCAG CTGCA^G
932 CTGCAG
SmlI 111 CTTGAG C^T[CT][GA]AG
1649 CTCAAG
Tth111I 365 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
827 GACATTGTC
Van91I 1400 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1525 CCAGCATCTGG
XcmI 952 CCAGGAGTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1432 CCAGCCTAGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI