##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
BanI BbeI BbrPI BcgI BclI
BfrI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HindIII KasI KpnI KspI
MaeII MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 1056 ACATGT A^C[GA][CT]GT
Alw44I 2164 GTGCAC G^TGCAC
AocI 116 CCTCAGG CC^T[ACGT]AGG
ApaLI 2164 GTGCAC G^TGCAC
AvrII 1463 CCTAGG C^CTAGG
BalI 1492 TGGCCA TGG^CCA
BamHI 233 GGATCC G^GATCC
BciVI 1508 GTATCC GTATCC(6/5)
BglI 2578 GCCATAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1463 CCTAGG C^CTAGG
BsaI 207 GGTCTC GGTCTC(1/5)
BsmBI 1049 GAGACG CGTCTC(1/5)
BspLU11I 1056 ACATGT A^CATGT
BspMI 1268 ACCTGC ACCTGC(4/8)
BsrDI 393 GCAATG GCAATG(2/0)
BssSI 1046 CACGAG CACGAG(-5/-1)
BstAPI 322 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 116 CCTCAGG CC^T[ACGT]AGG
CfoI 907 GCGC GCG^C
DrdI 1191 GACTGTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1238 CCATGG C^C[GA][CT]GG
Eco81I 116 CCTCAGG CC^T[ACGT]AGG
EcoRI 2301 GAATTC G^AATTC
HhaI 907 GCGC GCG^C
HinP1I 907 GCGC G^CGC
HincII 82 GTTAAC GT[CT]^[GA]AC
HindII 82 GTTAAC GT[CT]^[GA]AC
HpaI 82 GTTAAC GTT^AAC
MluNI 1492 TGGCCA TGG^CCA
MscI 1492 TGGCCA TGG^CCA
NcoI 1238 CCATGG C^CATGG
PstI 425 CTGCAG CTGCA^G
SapI 592 GAAGAGC GCTCTTC(1/4)
StuI 1524 AGGCCT AGG^CCT
TaqI 1077 TCGA T^CGA
TthHB8I 1077 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1856 GAATTT [GA]^AATT[CT]
2301 GAATTC
AfaI 1030 GTAC GT^AC
2320 GTAC
ApaI 579 GGGCCC GGGCC^C
1325 GGGCCC
ApoI 1856 GAATTT [GA]^AATT[CT]
2301 GAATTC
BbsI 811 GAAGAC GAAGAC(2/6)
1393 GAAGAC
BbvCI 1215 GCTGAGG CCTCAGC(-5/-2)
1228 GCTGAGG
BlpI 521 GCTAAGC GC^T[ACGT]AGC
2616 GCTCAGC
Bpu1102I 521 GCTAAGC GC^T[ACGT]AGC
2616 GCTCAGC
BpuAI 811 GAAGAC GAAGAC(2/6)
1393 GAAGAC
BsmI 1692 GCATTC GAATGC(1/-1)
1843 GCATTC
BsrBI 788 GAGCGG CCGCTC(-3/-3)
980 GAGCGG
CelII 521 GCTAAGC GC^T[ACGT]AGC
2616 GCTCAGC
DraIII 340 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2274 CACAAGGTG
EaeI 1330 CGGCCA [CT]^GGCC[GA]
1492 TGGCCA
EarI 592 GAAGAG CTCTTC(1/4)
950 GAAGAG
FauI 771 CCCGC CCCGC(4/6)
2046 CCCGC
Ksp632I 592 GAAGAG CTCTTC(1/4)
950 GAAGAG
MslI 1359 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1484 CACAGGGGTG
RsaI 1030 GTAC GT^AC
2320 GTAC
SfcI 425 CTGCAG C^T[GA][CT]AG
2230 CTGTAG
Tsp509I 1857 AATT ^AATT
2302 AATT
XbaI 1182 TCTAGA T^CTAGA
2140 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaBI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MamI MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TfiI Tru9I TseI Tsp45I
TspRI Van91I XcmI XhoII