################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BanI BbeI BbrPI BcgI BclI BfrI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII KasI KpnI KspI MaeII MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflIII 1056 ACATGT A^C[GA][CT]GT Alw44I 2164 GTGCAC G^TGCAC AocI 116 CCTCAGG CC^T[ACGT]AGG ApaLI 2164 GTGCAC G^TGCAC AvrII 1463 CCTAGG C^CTAGG BalI 1492 TGGCCA TGG^CCA BamHI 233 GGATCC G^GATCC BciVI 1508 GTATCC GTATCC(6/5) BglI 2578 GCCATAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1463 CCTAGG C^CTAGG BsaI 207 GGTCTC GGTCTC(1/5) BsmBI 1049 GAGACG CGTCTC(1/5) BspLU11I 1056 ACATGT A^CATGT BspMI 1268 ACCTGC ACCTGC(4/8) BsrDI 393 GCAATG GCAATG(2/0) BssSI 1046 CACGAG CACGAG(-5/-1) BstAPI 322 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 116 CCTCAGG CC^T[ACGT]AGG CfoI 907 GCGC GCG^C DrdI 1191 GACTGTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1238 CCATGG C^C[GA][CT]GG Eco81I 116 CCTCAGG CC^T[ACGT]AGG EcoRI 2301 GAATTC G^AATTC HhaI 907 GCGC GCG^C HinP1I 907 GCGC G^CGC HincII 82 GTTAAC GT[CT]^[GA]AC HindII 82 GTTAAC GT[CT]^[GA]AC HpaI 82 GTTAAC GTT^AAC MluNI 1492 TGGCCA TGG^CCA MscI 1492 TGGCCA TGG^CCA NcoI 1238 CCATGG C^CATGG PstI 425 CTGCAG CTGCA^G SapI 592 GAAGAGC GCTCTTC(1/4) StuI 1524 AGGCCT AGG^CCT TaqI 1077 TCGA T^CGA TthHB8I 1077 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 1856 GAATTT [GA]^AATT[CT] 2301 GAATTC AfaI 1030 GTAC GT^AC 2320 GTAC ApaI 579 GGGCCC GGGCC^C 1325 GGGCCC ApoI 1856 GAATTT [GA]^AATT[CT] 2301 GAATTC BbsI 811 GAAGAC GAAGAC(2/6) 1393 GAAGAC BbvCI 1215 GCTGAGG CCTCAGC(-5/-2) 1228 GCTGAGG BlpI 521 GCTAAGC GC^T[ACGT]AGC 2616 GCTCAGC Bpu1102I 521 GCTAAGC GC^T[ACGT]AGC 2616 GCTCAGC BpuAI 811 GAAGAC GAAGAC(2/6) 1393 GAAGAC BsmI 1692 GCATTC GAATGC(1/-1) 1843 GCATTC BsrBI 788 GAGCGG CCGCTC(-3/-3) 980 GAGCGG CelII 521 GCTAAGC GC^T[ACGT]AGC 2616 GCTCAGC DraIII 340 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG 2274 CACAAGGTG EaeI 1330 CGGCCA [CT]^GGCC[GA] 1492 TGGCCA EarI 592 GAAGAG CTCTTC(1/4) 950 GAAGAG FauI 771 CCCGC CCCGC(4/6) 2046 CCCGC Ksp632I 592 GAAGAG CTCTTC(1/4) 950 GAAGAG MslI 1359 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1484 CACAGGGGTG RsaI 1030 GTAC GT^AC 2320 GTAC SfcI 425 CTGCAG C^T[GA][CT]AG 2230 CTGTAG Tsp509I 1857 AATT ^AATT 2302 AATT XbaI 1182 TCTAGA T^CTAGA 2140 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaII BanII BbvI BcnI BfaI BglII BmyI BpmI BsaBI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MamI MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp45I TspRI Van91I XcmI XhoII