##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
BanI        BbeI        BbrPI       BcgI        BclI        
BfrI        BsaAI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     KasI        KpnI        KspI        
MaeII       MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AflIII       1056  ACATGT               A^C[GA][CT]GT
Alw44I       2164  GTGCAC               G^TGCAC
AocI          116  CCTCAGG              CC^T[ACGT]AGG
ApaLI        2164  GTGCAC               G^TGCAC
AvrII        1463  CCTAGG               C^CTAGG
BalI         1492  TGGCCA               TGG^CCA
BamHI         233  GGATCC               G^GATCC
BciVI        1508  GTATCC               GTATCC(6/5)
BglI         2578  GCCATAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1463  CCTAGG               C^CTAGG
BsaI          207  GGTCTC               GGTCTC(1/5)
BsmBI        1049  GAGACG               CGTCTC(1/5)
BspLU11I     1056  ACATGT               A^CATGT
BspMI        1268  ACCTGC               ACCTGC(4/8)
BsrDI         393  GCAATG               GCAATG(2/0)
BssSI        1046  CACGAG               CACGAG(-5/-1)
BstAPI        322  GCAACAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        116  CCTCAGG              CC^T[ACGT]AGG
CfoI          907  GCGC                 GCG^C
DrdI         1191  GACTGTATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1238  CCATGG               C^C[GA][CT]GG
Eco81I        116  CCTCAGG              CC^T[ACGT]AGG
EcoRI        2301  GAATTC               G^AATTC
HhaI          907  GCGC                 GCG^C
HinP1I        907  GCGC                 G^CGC
HincII         82  GTTAAC               GT[CT]^[GA]AC
HindII         82  GTTAAC               GT[CT]^[GA]AC
HpaI           82  GTTAAC               GTT^AAC
MluNI        1492  TGGCCA               TGG^CCA
MscI         1492  TGGCCA               TGG^CCA
NcoI         1238  CCATGG               C^CATGG
PstI          425  CTGCAG               CTGCA^G
SapI          592  GAAGAGC              GCTCTTC(1/4)
StuI         1524  AGGCCT               AGG^CCT
TaqI         1077  TCGA                 T^CGA
TthHB8I      1077  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI         1856  GAATTT               [GA]^AATT[CT]
             2301  GAATTC              
AfaI         1030  GTAC                 GT^AC
             2320  GTAC                
ApaI          579  GGGCCC               GGGCC^C
             1325  GGGCCC              
ApoI         1856  GAATTT               [GA]^AATT[CT]
             2301  GAATTC              
BbsI          811  GAAGAC               GAAGAC(2/6)
             1393  GAAGAC              
BbvCI        1215  GCTGAGG              CCTCAGC(-5/-2)
             1228  GCTGAGG             
BlpI          521  GCTAAGC              GC^T[ACGT]AGC
             2616  GCTCAGC             
Bpu1102I      521  GCTAAGC              GC^T[ACGT]AGC
             2616  GCTCAGC             
BpuAI         811  GAAGAC               GAAGAC(2/6)
             1393  GAAGAC              
BsmI         1692  GCATTC               GAATGC(1/-1)
             1843  GCATTC              
BsrBI         788  GAGCGG               CCGCTC(-3/-3)
              980  GAGCGG              
CelII         521  GCTAAGC              GC^T[ACGT]AGC
             2616  GCTCAGC             
DraIII        340  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2274  CACAAGGTG           
EaeI         1330  CGGCCA               [CT]^GGCC[GA]
             1492  TGGCCA              
EarI          592  GAAGAG               CTCTTC(1/4)
              950  GAAGAG              
FauI          771  CCCGC                CCCGC(4/6)
             2046  CCCGC               
Ksp632I       592  GAAGAG               CTCTTC(1/4)
              950  GAAGAG              
MslI         1359  CATAATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1484  CACAGGGGTG          
RsaI         1030  GTAC                 GT^AC
             2320  GTAC                
SfcI          425  CTGCAG               C^T[GA][CT]AG
             2230  CTGTAG              
Tsp509I      1857  AATT                 ^AATT
             2302  AATT                
XbaI         1182  TCTAGA               T^CTAGA
             2140  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AspHI       
AvaII       BanII       BbvI        BcnI        BfaI        
BglII       BmyI        BpmI        BsaBI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MamI        MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       Van91I      XcmI        XhoII