##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BalI BbeI BbrPI BciVI
BglI BglII BsaAI BsaI BseAI
BsiEI BsiWI BsmBI BsmI BspEI
BspHI BsrBI BssHII BssSI BstBI
BstEII BstPI BstUI CfoI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI HinP1I KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI Van91I XbaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1921 GACGTC GACGT^C
AcyI 1921 GACGTC G[GA]^CG[CT]C
AflIII 2304 ACATGT A^C[GA][CT]GT
AspI 352 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2480 CTCGAG C^[CT]CG[GA]G
AvrII 1641 CCTAGG C^CTAGG
BamHI 216 GGATCC G^GATCC
BbvCI 856 CCTCAGC CCTCAGC(-5/-2)
BcgI 102 CGATCCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1641 CCTAGG C^CTAGG
BlpI 393 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 393 GCTTAGC GC^T[ACGT]AGC
BsaBI 1027 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1921 GACGTC G[GA]^CG[CT]C
BsaWI 951 ACCGGA [AT]^CCGG[AT]
BsoBI 2480 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1384 TGTACA T^GTACA
BspDI 703 ATCGAT AT^CGAT
BspLU11I 2304 ACATGT A^CATGT
BsrDI 1245 CATTGC GCAATG(2/0)
BsrFI 521 ACCGGC [GA]^CCGG[CT]
BsrGI 1384 TGTACA T^GTACA
Bst1107I 2403 GTATAC GTA^TAC
BstAPI 2073 GCAAACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1722 CCAACGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 216 GGATCC [GA]^GATC[CT]
BstZ17I 2403 GTATAC GTA^TAC
CelII 393 GCTTAGC GC^T[ACGT]AGC
Cfr10I 521 ACCGGC [GA]^CCGG[CT]
ClaI 703 ATCGAT AT^CGAT
DraII 1882 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 185 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1882 AGGACCT [GA]G^G[ACGT]CC[CT]
Hin1I 1921 GACGTC G[GA]^CG[CT]C
HpaI 1733 GTTAAC GTT^AAC
HphI 1024 GGTGA GGTGA(8/7)
MamI 1027 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 216 GGATCC [GA]^GATC[CT]
MspA1I 925 CAGCTG C[AC]G^C[GT]G
NcoI 2544 CCATGG C^CATGG
PaeR7I 2480 CTCGAG C^TCGAG
PflFI 352 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1882 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1951 CTGCAG CTGCA^G
PvuII 925 CAGCTG CAG^CTG
ScaI 2125 AGTACT AGT^ACT
SpeI 1314 ACTAGT A^CTAGT
SspBI 1384 TGTACA T^GTACA
Tth111I 352 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2480 CTCGAG C^TCGAG
XhoII 216 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1924 GTCTAC GT^[AC][GT]AC
2403 GTATAC
AcsI 1815 GAATTT [GA]^AATT[CT]
2439 AAATTT
AflII 1192 CTTAAG C^TTAAG
2347 CTTAAG
AlwNI 505 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1945 CAGGGGCTG
AocI 573 CCTTAGG CC^T[ACGT]AGG
1942 CCTCAGG
ApoI 1815 GAATTT [GA]^AATT[CT]
2439 AAATTT
Asp700I 531 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1862 GAAATCATTC
BanI 1345 GGCACC G^G[CT][GA]CC
1590 GGCACC
BcnI 824 CCGGG CC^[GC]GG
933 CCCGG
BfrI 1192 CTTAAG C^TTAAG
2347 CTTAAG
Bsu36I 573 CCTTAGG CC^T[ACGT]AGG
1942 CCTCAGG
DsaI 1261 CCACGG C^C[GA][CT]GG
2544 CCATGG
Eco81I 573 CCTTAGG CC^T[ACGT]AGG
1942 CCTCAGG
EcoNI 2022 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2236 CCTAATAAAGG
HaeIII 194 GGCC GG^CC
756 GGCC
HindIII 278 AAGCTT A^AGCTT
448 AAGCTT
MaeII 1922 ACGT A^CGT
2532 ACGT
MslI 1615 CATTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2359 CACTTTTATG
NciI 824 CCGGG CC^[GC]GG
933 CCCGG
NspI 1560 GCATGT [GA]CATG^[CT]
2304 ACATGT
XmnI 531 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1862 GAAATCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbsI BbvI BclI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I FbaI Fnu4HI FokI HapII
HincII HindII HinfI HpaII ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I