################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## Acc65I AccII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII BalI BbeI BbrPI BciVI BglI BglII BsaAI BsaI BseAI BsiEI BsiWI BsmBI BsmI BspEI BspHI BsrBI BssHII BssSI BstBI BstEII BstPI BstUI CfoI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI HinP1I KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1921 GACGTC GACGT^C AcyI 1921 GACGTC G[GA]^CG[CT]C AflIII 2304 ACATGT A^C[GA][CT]GT AspI 352 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2480 CTCGAG C^[CT]CG[GA]G AvrII 1641 CCTAGG C^CTAGG BamHI 216 GGATCC G^GATCC BbvCI 856 CCTCAGC CCTCAGC(-5/-2) BcgI 102 CGATCCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 1641 CCTAGG C^CTAGG BlpI 393 GCTTAGC GC^T[ACGT]AGC Bpu1102I 393 GCTTAGC GC^T[ACGT]AGC BsaBI 1027 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1921 GACGTC G[GA]^CG[CT]C BsaWI 951 ACCGGA [AT]^CCGG[AT] BsoBI 2480 CTCGAG C^[CT]CG[GA]G Bsp1407I 1384 TGTACA T^GTACA BspDI 703 ATCGAT AT^CGAT BspLU11I 2304 ACATGT A^CATGT BsrDI 1245 CATTGC GCAATG(2/0) BsrFI 521 ACCGGC [GA]^CCGG[CT] BsrGI 1384 TGTACA T^GTACA Bst1107I 2403 GTATAC GTA^TAC BstAPI 2073 GCAAACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1722 CCAACGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 216 GGATCC [GA]^GATC[CT] BstZ17I 2403 GTATAC GTA^TAC CelII 393 GCTTAGC GC^T[ACGT]AGC Cfr10I 521 ACCGGC [GA]^CCGG[CT] ClaI 703 ATCGAT AT^CGAT DraII 1882 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 185 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 1882 AGGACCT [GA]G^G[ACGT]CC[CT] Hin1I 1921 GACGTC G[GA]^CG[CT]C HpaI 1733 GTTAAC GTT^AAC HphI 1024 GGTGA GGTGA(8/7) MamI 1027 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 216 GGATCC [GA]^GATC[CT] MspA1I 925 CAGCTG C[AC]G^C[GT]G NcoI 2544 CCATGG C^CATGG PaeR7I 2480 CTCGAG C^TCGAG PflFI 352 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1882 AGGACCT [GA]G^G[AT]CC[CT] PstI 1951 CTGCAG CTGCA^G PvuII 925 CAGCTG CAG^CTG ScaI 2125 AGTACT AGT^ACT SpeI 1314 ACTAGT A^CTAGT SspBI 1384 TGTACA T^GTACA Tth111I 352 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2480 CTCGAG C^TCGAG XhoII 216 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 1924 GTCTAC GT^[AC][GT]AC 2403 GTATAC AcsI 1815 GAATTT [GA]^AATT[CT] 2439 AAATTT AflII 1192 CTTAAG C^TTAAG 2347 CTTAAG AlwNI 505 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1945 CAGGGGCTG AocI 573 CCTTAGG CC^T[ACGT]AGG 1942 CCTCAGG ApoI 1815 GAATTT [GA]^AATT[CT] 2439 AAATTT Asp700I 531 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1862 GAAATCATTC BanI 1345 GGCACC G^G[CT][GA]CC 1590 GGCACC BcnI 824 CCGGG CC^[GC]GG 933 CCCGG BfrI 1192 CTTAAG C^TTAAG 2347 CTTAAG Bsu36I 573 CCTTAGG CC^T[ACGT]AGG 1942 CCTCAGG DsaI 1261 CCACGG C^C[GA][CT]GG 2544 CCATGG Eco81I 573 CCTTAGG CC^T[ACGT]AGG 1942 CCTCAGG EcoNI 2022 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2236 CCTAATAAAGG HaeIII 194 GGCC GG^CC 756 GGCC HindIII 278 AAGCTT A^AGCTT 448 AAGCTT MaeII 1922 ACGT A^CGT 2532 ACGT MslI 1615 CATTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2359 CACTTTTATG NciI 824 CCGGG CC^[GC]GG 933 CCCGG NspI 1560 GCATGT [GA]CATG^[CT] 2304 ACATGT XmnI 531 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1862 GAAATCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbsI BbvI BclI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I FbaI Fnu4HI FokI HapII HincII HindII HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I