##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccI AclI AflII AgeI Aor51HI
AscI AviII BamHI BbsI BcgI
BciVI BclI BfrI BlpI Bpu1102I
BpuAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI MfeI
MluI MunI NcoI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI SwaI TaqI TthHB8I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 741 GACGTC GACGT^C
AccII 814 CGCG CG^CG
AccIII 384 TCCGGA T^CCGGA
AcsI 62 GAATTC [GA]^AATT[CT]
AflIII 861 ACACGT A^C[GA][CT]GT
AhdI 2103 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1711 GTGCAC G^TGCAC
ApaLI 1711 GTGCAC G^TGCAC
ApoI 62 GAATTC [GA]^AATT[CT]
AseI 2768 ATTAAT AT^TAAT
AsnI 2768 ATTAAT AT^TAAT
Asp700I 1604 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2103 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 434 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 27 GGCGCC GGCGC^C
BbrPI 2688 CACGTG CAC^GTG
BglI 241 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 905 AGATCT A^GATCT
BsaBI 2156 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 384 TCCGGA [AT]^CCGG[AT]
BseAI 384 TCCGGA T^CCGGA
BsmBI 743 CGTCTC CGTCTC(1/5)
BspEI 384 TCCGGA T^CCGGA
BspHI 1955 TCATGA T^CATGA
BsrFI 2234 GCCGGC [GA]^CCGG[CT]
BstAPI 257 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 814 CGCG CG^CG
Cfr10I 2234 GCCGGC [GA]^CCGG[CT]
Eam1105I 2103 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2277 CCTCTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 62 GAATTC G^AATTC
HaeII 27 GGCGCC [GA]GCGC^[CT]
KasI 27 GGCGCC G^GCGCC
KspI 813 CCGCGG CCGC^GG
MamI 2156 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 384 TCCGGA T^CCGGA
MseI 2769 TTAA T^TAA
MvnI 814 CGCG CG^CG
NaeI 2234 GCCGGC GCC^GGC
NarI 27 GGCGCC GG^CGCC
NdeI 2638 CATATG CA^TATG
NgoMIV 2234 GCCGGC G^CCGGC
PflFI 434 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2688 CACGTG CAC^GTG
PmlI 2688 CACGTG CAC^GTG
PshBI 2768 ATTAAT AT^TAAT
RcaI 1955 TCATGA T^CATGA
SacII 813 CCGCGG CCGC^GG
SfoI 27 GGCGCC GGC^GCC
SmaI 2328 CCCGGG CCC^GGG
SmlI 495 CTCAAG C^T[CT][GA]AG
SspI 2184 AATATT AAT^ATT
Tru9I 2769 TTAA T^TAA
Tth111I 434 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2328 CCCGGG C^CCGGG
XmaI 2328 CCCGGG C^CCGGG
XmnI 1604 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 232 GGTACC G^GTACC
509 GGTACC
AcyI 27 GGCGCC G[GA]^CG[CT]C
741 GACGTC
Asp718I 232 GGTACC G^GTACC
509 GGTACC
AvaI 316 CTCGGG C^[CT]CG[GA]G
2328 CCCGGG
BpmI 793 CTCCAG CTGGAG(16/14)
579 CTGGAG
BsaAI 862 CACGTA [CT]AC^GT[GA]
2688 CACGTG
BsaHI 27 GGCGCC G[GA]^CG[CT]C
741 GACGTC
BsaI 2049 GAGACC GGTCTC(1/5)
568 GGTCTC
BsoBI 316 CTCGGG C^[CT]CG[GA]G
2328 CCCGGG
BspMI 1173 ACCTGC ACCTGC(4/8)
1786 ACCTGC
BsrDI 585 CATTGC GCAATG(2/0)
1523 CATTGC
BstEII 935 GGTGACC G^GT[ACGT]ACC
2110 GGTCACC
BstPI 935 GGTGACC G^GT[ACGT]ACC
2110 GGTCACC
CfoI 28 GCGC GCG^C
2602 GCGC
EarI 86 CTCTTC CTCTTC(1/4)
2758 GAAGAG
EcoO65I 935 GGTGACC G^GT[ACGT]ACC
2110 GGTCACC
HhaI 28 GCGC GCG^C
2602 GCGC
Hin1I 27 GGCGCC G[GA]^CG[CT]C
741 GACGTC
HinP1I 28 GCGC G^CGC
2602 GCGC
KpnI 232 GGTACC GGTAC^C
509 GGTACC
Ksp632I 86 CTCTTC CTCTTC(1/4)
2758 GAAGAG
NspI 1389 ACATGC [GA]CATG^[CT]
1697 GCATGT
PvuII 1854 CAGCTG CAG^CTG
1999 CAGCTG
SacI 323 GAGCTC GAGCT^C
492 GAGCTC
SexAI 228 ACCTGGT A^CC[AT]GGT
435 ACCTGGT
SpeI 155 ACTAGT A^CTAGT
2646 ACTAGT
StuI 499 AGGCCT AGG^CCT
1574 AGGCCT
TfiI 979 GAATC G^A[AT]TC
1959 GATTC
Tsp509I 63 AATT ^AATT
2767 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaII AvrII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BlnI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NheI NlaIII NlaIV PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI Van91I XcmI
XhoII