################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AccI AclI AflII AgeI Aor51HI AscI AviII BamHI BbsI BcgI BciVI BclI BfrI BlpI Bpu1102I BpuAI BsiEI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI MfeI MluI MunI NcoI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspBI SwaI TaqI TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 741 GACGTC GACGT^C AccII 814 CGCG CG^CG AccIII 384 TCCGGA T^CCGGA AcsI 62 GAATTC [GA]^AATT[CT] AflIII 861 ACACGT A^C[GA][CT]GT AhdI 2103 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1711 GTGCAC G^TGCAC ApaLI 1711 GTGCAC G^TGCAC ApoI 62 GAATTC [GA]^AATT[CT] AseI 2768 ATTAAT AT^TAAT AsnI 2768 ATTAAT AT^TAAT Asp700I 1604 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2103 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 434 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 27 GGCGCC GGCGC^C BbrPI 2688 CACGTG CAC^GTG BglI 241 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 905 AGATCT A^GATCT BsaBI 2156 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 384 TCCGGA [AT]^CCGG[AT] BseAI 384 TCCGGA T^CCGGA BsmBI 743 CGTCTC CGTCTC(1/5) BspEI 384 TCCGGA T^CCGGA BspHI 1955 TCATGA T^CATGA BsrFI 2234 GCCGGC [GA]^CCGG[CT] BstAPI 257 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 814 CGCG CG^CG Cfr10I 2234 GCCGGC [GA]^CCGG[CT] Eam1105I 2103 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2277 CCTCTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 62 GAATTC G^AATTC HaeII 27 GGCGCC [GA]GCGC^[CT] KasI 27 GGCGCC G^GCGCC KspI 813 CCGCGG CCGC^GG MamI 2156 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 384 TCCGGA T^CCGGA MseI 2769 TTAA T^TAA MvnI 814 CGCG CG^CG NaeI 2234 GCCGGC GCC^GGC NarI 27 GGCGCC GG^CGCC NdeI 2638 CATATG CA^TATG NgoMIV 2234 GCCGGC G^CCGGC PflFI 434 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2688 CACGTG CAC^GTG PmlI 2688 CACGTG CAC^GTG PshBI 2768 ATTAAT AT^TAAT RcaI 1955 TCATGA T^CATGA SacII 813 CCGCGG CCGC^GG SfoI 27 GGCGCC GGC^GCC SmaI 2328 CCCGGG CCC^GGG SmlI 495 CTCAAG C^T[CT][GA]AG SspI 2184 AATATT AAT^ATT Tru9I 2769 TTAA T^TAA Tth111I 434 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2328 CCCGGG C^CCGGG XmaI 2328 CCCGGG C^CCGGG XmnI 1604 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 232 GGTACC G^GTACC 509 GGTACC AcyI 27 GGCGCC G[GA]^CG[CT]C 741 GACGTC Asp718I 232 GGTACC G^GTACC 509 GGTACC AvaI 316 CTCGGG C^[CT]CG[GA]G 2328 CCCGGG BpmI 793 CTCCAG CTGGAG(16/14) 579 CTGGAG BsaAI 862 CACGTA [CT]AC^GT[GA] 2688 CACGTG BsaHI 27 GGCGCC G[GA]^CG[CT]C 741 GACGTC BsaI 2049 GAGACC GGTCTC(1/5) 568 GGTCTC BsoBI 316 CTCGGG C^[CT]CG[GA]G 2328 CCCGGG BspMI 1173 ACCTGC ACCTGC(4/8) 1786 ACCTGC BsrDI 585 CATTGC GCAATG(2/0) 1523 CATTGC BstEII 935 GGTGACC G^GT[ACGT]ACC 2110 GGTCACC BstPI 935 GGTGACC G^GT[ACGT]ACC 2110 GGTCACC CfoI 28 GCGC GCG^C 2602 GCGC EarI 86 CTCTTC CTCTTC(1/4) 2758 GAAGAG EcoO65I 935 GGTGACC G^GT[ACGT]ACC 2110 GGTCACC HhaI 28 GCGC GCG^C 2602 GCGC Hin1I 27 GGCGCC G[GA]^CG[CT]C 741 GACGTC HinP1I 28 GCGC G^CGC 2602 GCGC KpnI 232 GGTACC GGTAC^C 509 GGTACC Ksp632I 86 CTCTTC CTCTTC(1/4) 2758 GAAGAG NspI 1389 ACATGC [GA]CATG^[CT] 1697 GCATGT PvuII 1854 CAGCTG CAG^CTG 1999 CAGCTG SacI 323 GAGCTC GAGCT^C 492 GAGCTC SexAI 228 ACCTGGT A^CC[AT]GGT 435 ACCTGGT SpeI 155 ACTAGT A^CTAGT 2646 ACTAGT StuI 499 AGGCCT AGG^CCT 1574 AGGCCT TfiI 979 GAATC G^A[AT]TC 1959 GATTC Tsp509I 63 AATT ^AATT 2767 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaII AvrII BalI BanI BanII BbvCI BbvI BcnI BfaI BlnI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NheI NlaIII NlaIV PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI Van91I XcmI XhoII