##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AccI        AclI        AflII       AgeI        Aor51HI     
AscI        AviII       BamHI       BbsI        BcgI        
BciVI       BclI        BfrI        BlpI        Bpu1102I    
BpuAI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspLU11I    BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
HincII      HindII      HindIII     HpaI        MfeI        
MluI        MunI        NcoI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       Psp1406I    PvuI        RsrII       
SalI        SapI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SphI        Sse8387I    
SspBI       SwaI        TaqI        TthHB8I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII         741  GACGTC               GACGT^C
AccII         814  CGCG                 CG^CG
AccIII        384  TCCGGA               T^CCGGA
AcsI           62  GAATTC               [GA]^AATT[CT]
AflIII        861  ACACGT               A^C[GA][CT]GT
AhdI         2103  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1711  GTGCAC               G^TGCAC
ApaLI        1711  GTGCAC               G^TGCAC
ApoI           62  GAATTC               [GA]^AATT[CT]
AseI         2768  ATTAAT               AT^TAAT
AsnI         2768  ATTAAT               AT^TAAT
Asp700I      1604  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2103  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          434  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI           27  GGCGCC               GGCGC^C
BbrPI        2688  CACGTG               CAC^GTG
BglI          241  GCCCCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         905  AGATCT               A^GATCT
BsaBI        2156  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         384  TCCGGA               [AT]^CCGG[AT]
BseAI         384  TCCGGA               T^CCGGA
BsmBI         743  CGTCTC               CGTCTC(1/5)
BspEI         384  TCCGGA               T^CCGGA
BspHI        1955  TCATGA               T^CATGA
BsrFI        2234  GCCGGC               [GA]^CCGG[CT]
BstAPI        257  GCAGGATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         814  CGCG                 CG^CG
Cfr10I       2234  GCCGGC               [GA]^CCGG[CT]
Eam1105I     2103  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2277  CCTCTGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI          62  GAATTC               G^AATTC
HaeII          27  GGCGCC               [GA]GCGC^[CT]
KasI           27  GGCGCC               G^GCGCC
KspI          813  CCGCGG               CCGC^GG
MamI         2156  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          384  TCCGGA               T^CCGGA
MseI         2769  TTAA                 T^TAA
MvnI          814  CGCG                 CG^CG
NaeI         2234  GCCGGC               GCC^GGC
NarI           27  GGCGCC               GG^CGCC
NdeI         2638  CATATG               CA^TATG
NgoMIV       2234  GCCGGC               G^CCGGC
PflFI         434  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2688  CACGTG               CAC^GTG
PmlI         2688  CACGTG               CAC^GTG
PshBI        2768  ATTAAT               AT^TAAT
RcaI         1955  TCATGA               T^CATGA
SacII         813  CCGCGG               CCGC^GG
SfoI           27  GGCGCC               GGC^GCC
SmaI         2328  CCCGGG               CCC^GGG
SmlI          495  CTCAAG               C^T[CT][GA]AG
SspI         2184  AATATT               AAT^ATT
Tru9I        2769  TTAA                 T^TAA
Tth111I       434  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2328  CCCGGG               C^CCGGG
XmaI         2328  CCCGGG               C^CCGGG
XmnI         1604  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

Acc65I        232  GGTACC               G^GTACC
              509  GGTACC              
AcyI           27  GGCGCC               G[GA]^CG[CT]C
              741  GACGTC              
Asp718I       232  GGTACC               G^GTACC
              509  GGTACC              
AvaI          316  CTCGGG               C^[CT]CG[GA]G
             2328  CCCGGG              
BpmI          793  CTCCAG               CTGGAG(16/14)
              579  CTGGAG              
BsaAI         862  CACGTA               [CT]AC^GT[GA]
             2688  CACGTG              
BsaHI          27  GGCGCC               G[GA]^CG[CT]C
              741  GACGTC              
BsaI         2049  GAGACC               GGTCTC(1/5)
              568  GGTCTC              
BsoBI         316  CTCGGG               C^[CT]CG[GA]G
             2328  CCCGGG              
BspMI        1173  ACCTGC               ACCTGC(4/8)
             1786  ACCTGC              
BsrDI         585  CATTGC               GCAATG(2/0)
             1523  CATTGC              
BstEII        935  GGTGACC              G^GT[ACGT]ACC
             2110  GGTCACC             
BstPI         935  GGTGACC              G^GT[ACGT]ACC
             2110  GGTCACC             
CfoI           28  GCGC                 GCG^C
             2602  GCGC                
EarI           86  CTCTTC               CTCTTC(1/4)
             2758  GAAGAG              
EcoO65I       935  GGTGACC              G^GT[ACGT]ACC
             2110  GGTCACC             
HhaI           28  GCGC                 GCG^C
             2602  GCGC                
Hin1I          27  GGCGCC               G[GA]^CG[CT]C
              741  GACGTC              
HinP1I         28  GCGC                 G^CGC
             2602  GCGC                
KpnI          232  GGTACC               GGTAC^C
              509  GGTACC              
Ksp632I        86  CTCTTC               CTCTTC(1/4)
             2758  GAAGAG              
NspI         1389  ACATGC               [GA]CATG^[CT]
             1697  GCATGT              
PvuII        1854  CAGCTG               CAG^CTG
             1999  CAGCTG              
SacI          323  GAGCTC               GAGCT^C
              492  GAGCTC              
SexAI         228  ACCTGGT              A^CC[AT]GGT
              435  ACCTGGT             
SpeI          155  ACTAGT               A^CTAGT
             2646  ACTAGT              
StuI          499  AGGCCT               AGG^CCT
             1574  AGGCCT              
TfiI          979  GAATC                G^A[AT]TC
             1959  GATTC               
Tsp509I        63  AATT                 ^AATT
             2767  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        ApaI        AspHI       AvaII       AvrII       
BalI        BanI        BanII       BbvCI       BbvI        
BcnI        BfaI        BlnI        BmyI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NheI        NlaIII      NlaIV       PflMI       
PleI        PpuMI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TseI        Tsp45I      TspRI       Van91I      XcmI        
XhoII